DNA measurements for monitoring chemotherapy in advanced head and neck carcinomas

Welkoborsky, Hans-J.; Mann, Wolf J.; Sanal, Cem; Freije, James E.

doi:10.1002/hed.2880160306

Cited by 10 publications

(8 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Quantitative DNA analysis was then performed using a computerized image analysis system (Cytometer CM1, Hund Cie., Wetzlar, Germany). At least 300 tumor cells were measured as described in detail in previous studies [13][14][15][16]. From the single-cell measurements, the following indices were derived [17][18][19][20][21]: (1) 2c deviation index (2cDI), defined as the mean square deviation of the DNA content from normal 2c; (2) 5c exceeding rate (5cER), defined as the percentage of cells with an aneuploid DNA content exceeding 5c.…”

Section: Identification Of Roi and Matching With Tissue Samplementioning

confidence: 99%

[Fluorine-18]Fluorodeoxyglucose Positron Emission Tomography, DNA Ploidy and Growth Fraction in Squamous-Cell Carcinomas of the Head and Neck

Jacob¹,

Welkoborsky²,

Mann³

et al. 2001

ORL

Self Cite

View full text Add to dashboard Cite

Background: Positron emission tomography (PET) offers an opportunity to examine noninvasively cellular functions with different tracers. [¹⁸F]Fluorodeoxyglucose (FDG) is most commonly used in identifying malignant tumors. Several tumor biologic characteristics (tumor cell viability, growth faction, treatment response to radiation, cell membrane dysfunction, recurrence rate) are suggested to be characterized by [¹⁸F]FDG PET. The aim of this study was to assess which other tumor biologic characteristics of squamous-cell carcinoma of the head and neck are correlated with [¹⁸F]FDG PET. Methods: [¹⁸F]FDG PET was performed in 14 patients with squamous-cell carcinomas of the upper digestive tract (TNM classification T₂–T₄, N₁–N₃). After attenuation correction, predefined areas of the tumor were semiquantitatively analyzed by the technique of the region of interest and calculated as standard uptake values (SUV). Afterwards, 5 biopsies of different tumor regions were obtained during endoscopy in each patient under general anesthesia, and a correlation between SUV of [¹⁸F]FDG PET and tumor biologic parameters was attempted. These parameters included: quantitative DNA measurements (i.e. 2c deviation index, 5c exceeding rate), immunohistochemical assessment of growth fraction (i.e. Ki67-MIB-1, PCNA) along with morphological tumor front grading. Results: The results revealed a marked variation of proliferation and cellular differentiation in various regions of the tumor for all parameters examined. There was a close correlation between [¹⁸F]FDG uptake and growth fraction (r = 0.83 for Ki67-MIB-1 and r = 0.8 for PCNA). A poor correlation was found between DNA aneuploidy (r = 0.4) or tumor front grading (r = 0.12) and [¹⁸F]FDG uptake. Conclusions: Our results confirm previous clinical and histologic observations that squamous-cell carcinomas of the upper digestive tract are heterogeneous tumors. Ki67 antigen, which has been shown to be of predictive value for proliferation and individual prognosis, correlated with [¹⁸F]FDG uptake. Using [¹⁸F]FDG PET, the main proliferation centers of inhomogeneous squamous-cell carcinomas could be identified with possible clinical implications for patient management.

show abstract

Section: Identification Of Roi and Matching With Tissue Samplementioning

confidence: 99%

[Fluorine-18]Fluorodeoxyglucose Positron Emission Tomography, DNA Ploidy and Growth Fraction in Squamous-Cell Carcinomas of the Head and Neck

Jacob¹,

Welkoborsky²,

Mann³

et al. 2001

ORL

Self Cite

View full text Add to dashboard Cite

show abstract

“…growth fraction, degree of DNA aneuploidy) [2,3,21,32]. Previous retrospective studies have shown that these parameters may be relevant independent of other clinical parameters (TNM stage) or location of the primary [14,16].…”

Section: Discussionmentioning

confidence: 99%

“…For quantitative DNA measurements [13], at least 300 tumor cells were measured at random after identification on the TV screen as reported in previous studies [5,[14][15][16]. From the single cell measurements, the following indices were derived [17]: (1) 2c deviation Index (2cDI) = mean square deviation of the DNA content from normal 2c; (2) 5c exceeding rate (5cER) = percentage of cells with an aneuploid DNA content exceeding 5c.…”

Section: Tumor Biologic Examinationsmentioning

confidence: 99%

Histological Grading, Growth Fraction and DNA-Ploidy as Criteria for the Treatment of Pharyngeal and Supraglottic Squamous Cell Carcinomas: A Preliminary, Prospective Study

Jacob¹,

Welkoborsky

Bittinger

et al. 2001

ORL

Self Cite

View full text Add to dashboard Cite

Background: Individualized treatment for patients with squamous cell carcinomas is most desirable. Biologic parameters of tumors may provide relevant criteria to achieve this goal. The aim of this study was: (1) to avoid elective neck dissection in patients with squamous cell carcinomas of the pharynx and supraglottis staged N0 by ultrasonography; (2) to administer postoperative radiotherapy only in patients staged ‘highly aggressive’ by biologic tumor parameters, in order to reduce patient morbidity and to improve quality of life. Study Design and Methods: This prospective trial was performed in 35 patients (any T stage, N0 or N1 by ultrasonographic examination). Tumor biopsies were taken for histological examination and for evaluation of some biological tumor markers. These parameters comprised DNA cytometric examinations, histological grading of the tumor front and immunohistochemical staining for proliferation markers (MIB1, PCNA). Based on these parameters, tumors were classified into ‘low aggressive’ (group 1) and highly aggressive (group 2) behavior. In all patients, second primaries were excluded by endoscopy, and the presenting tumor was resected with clear margins. In cases of ultrasonographically enlarged lymph nodes, functional neck dissection was performed. When biologic tumor profile showed low aggressive behavior (group 1), no postoperative radiotherapy was advised. In cases of highly aggressive tumors, radiotherapy was recommended postoperatively independent of all other parameters (group 2a and 2b). Results: The average follow-up was 36 months (range 16–65 months). Fifteen patients were included in group 1, 20 patients in group 2. Three patients with highly aggressive tumors who refused RTx were regarded independently (group 2b). In this pilot study of 35 patients, there was no difference in disease-free survival between group 1 and group 2a. Twenty-five of 35 patients were alive with no evidence of disease 29–65 months after treatment (3 died of disease; 5 died of second primary carcinomas; 2 died independent of disease). Eleven of 15 patients survived disease free in group 1, 13/17 in group 2a. In group 2b, 2 out of 3 patients, who were advised but refused postoperative radiotherapy, had recurrences. Conclusions: The results of this preliminary study point towards usefulness of some tumor biologic parameters in decision making for individualized treatment in patients with squamous cell carcinomas.

show abstract

“…Hemmer et al 7,8 showed that the application of DNA analysis contributed to a better understanding of the in-vivo effects of anticancer drugs for oral squamous cell carcinoma. Welkoborsky et al 9 suggested that changes in the DNA content after the first course of chemotherapy may correlate with the outcome of advanced head and neck carcinoma. Although it was revealed that DNA analysis was useful for monitoring chemotherapy in head and neck cancer, these two studies failed to fully define the relationship between the effects of chemotherapy and the changes in cellular DNA content brought about by the anticancer agents, or the clinical significance of these changes.…”

Section: Introductionmentioning

confidence: 99%

Control of cell proliferation kinetics of tumor in neoadjuvant chemotherapy for advanced oral squamous cell carcinoma and its prognostic implications

Noguchi

Kinjyo²,

Miyazaki³

et al. 2002

International Journal of Clinical Oncology

View full text Add to dashboard Cite

show abstract

DNA measurements for monitoring chemotherapy in advanced head and neck carcinomas

Cited by 10 publications

References 21 publications

[Fluorine-18]Fluorodeoxyglucose Positron Emission Tomography, DNA Ploidy and Growth Fraction in Squamous-Cell Carcinomas of the Head and Neck

[Fluorine-18]Fluorodeoxyglucose Positron Emission Tomography, DNA Ploidy and Growth Fraction in Squamous-Cell Carcinomas of the Head and Neck

Histological Grading, Growth Fraction and DNA-Ploidy as Criteria for the Treatment of Pharyngeal and Supraglottic Squamous Cell Carcinomas: A Preliminary, Prospective Study

Control of cell proliferation kinetics of tumor in neoadjuvant chemotherapy for advanced oral squamous cell carcinoma and its prognostic implications

Contact Info

Product

Resources

About