2021
DOI: 10.1002/pca.3026
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DNA meta‐barcoding using rbcL based mini‐barcode revealed presence of unspecified plant species in Ayurvedic polyherbal formulations

Abstract: Introduction Ayurveda takes advantage of the beneficial properties of medicinal plants. High demands in combination with inadequate availability of botanicals and a lack of knowledge with respect to their precise identification lead to adulterations in herbal products. Identification becomes more difficult in complex herbal formulations. Four different polyherbal formulations have been analyzed for the present paper. The targeted plants have different pharmacological properties for various ailments. Objective … Show more

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Cited by 13 publications
(25 citation statements)
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“…Reference plant materials were collected with the aid of a taxonomist from the Maharaja Sayajirao University (MSU), Vadodara (Gujarat, India) and the Directorate of Medicinal and Aromatic Plants Research (DMAPR), Anand (Gujarat, India). Reference plant materials were authenticated by Sanger sequencing of rbcL gene as described earlier 17 and sequences were submitted to the NCBI database (accession number MW628906 to MW628936). Voucher specimens were developed and deposited in our institutional herbarium.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Reference plant materials were collected with the aid of a taxonomist from the Maharaja Sayajirao University (MSU), Vadodara (Gujarat, India) and the Directorate of Medicinal and Aromatic Plants Research (DMAPR), Anand (Gujarat, India). Reference plant materials were authenticated by Sanger sequencing of rbcL gene as described earlier 17 and sequences were submitted to the NCBI database (accession number MW628906 to MW628936). Voucher specimens were developed and deposited in our institutional herbarium.…”
Section: Methodsmentioning
confidence: 99%
“…Therefore, the aims of the present study were: 1) to develop a new rbcL and ITS metabarcode primers for the detection of medicinal plant species 2) to validate the primers efficiency using mock controls and then apply the same for the market formulations (17 different single drugs and 15 polyherbal market formulations). 3) to see whether a multibarcoding approach could be used to detect targeted plant species in herbal formulations?…”
Section: Introductionmentioning
confidence: 99%
“…Chemical fingerprinting suffers from the fact that it is difficult to establish species-specific chemical markers, and the markers are sensitive to the age, season, and place of collection of the plant material [11]. With technological advancements, drastic reduction in sequencing cost, and increasing richness of reference sequences, DNA barcoding has emerged as a more versatile and robust method for authenticating herbal products and raw drugs through molecular species identification [12,13]. DNA barcoding uses the markers that are conserved within species but divergent between species so that species-specific sequences can be retrieved using a single pair of universal primers.…”
Section: Introductionmentioning
confidence: 99%
“…Presently, common methods of species identification include morphological analysis 5 , molecular marker technology 6 – 9 , protein electrophoresis 10 , liquid chromatography 11 , spectral analysis 12 14 and image recognition 15 . Morphological analysis requires a high level of expertise that is not easily acquired and as such, due to large morphological similarity between some species, the rate of accurate identification is low 16 .…”
Section: Introductionmentioning
confidence: 99%