2023
DOI: 10.1002/ece3.10649
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DNA metabarcoding assessment of Neotropical ichthyoplankton communities is marker‐dependent

Daniel Fonseca Teixeira,
Heron Oliveira Hilário,
Gilmar Bastos Santos
et al.

Abstract: The study of ichthyoplankton is paramount to understanding fish assemblages' reproductive dynamics. DNA metabarcoding has been applied as a rapid, cost‐effective, and accurate taxonomy tool, allowing the identification of multiple individuals simultaneously. However, there remain significant challenges when using DNA metabarcoding, such as molecular marker choice according to the taxonomic resolution and length of the fragment to be sequenced, primer bias, incomplete reference databases, and qualitative infere… Show more

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