DNA methylation analysis of sterile and fertile CMS-C hybrids and their parents in maize

Bo, Chen; Zhang, Yan; Lu, Yanli; Wang, Jing; Zhang, Suzhi; Long, Hai; Tang, Rong; Cao, Mengji

doi:10.1007/s13562-015-0298-6

Cited by 16 publications

(13 citation statements)

References 23 publications

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“…Some studies have found altered methylation patterns in male sterile plants and evidence that methylation regulates EGMS in rice [23,24,25], CMS in rice [26] and maize [27] and GMS in tomato [28] and wheat [29]. However, to date, no study has reported these methylation profiles and their possible roles in the cabbage male sterility line.…”

Section: Discussionmentioning

confidence: 99%

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Comparative Analysis of Genome Wide DNA Methylation Profiles for the Genic Male Sterile Cabbage Line 01-20S and Its Maintainer Line

Han

Zhang

Liu

et al. 2017

Genes

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Methylation modifications play an important role in multiple biological processes. Several studies have reported altered methylation patterns in male sterile plants such as rice and wheat, but little is known about the global methylation profiles and their possible roles in the cabbage (Brassica oleracea) male sterile line. In this study, single-base-resolution bisulfite sequencing (BS-Seq) was adopted to identify the pattern and degree of cytosine methylation in the male sterile line 01-20S and its near-isogenic fertile line 01-20F. Similar methylation patterns were profiled, with some changes observed in local positions. In total, 505 differentially methylated genomic regions (DMRs) and 106 DMR-associated genes were detected. Nine genes related to pollen development were discovered and further validated by a quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Among these, four were downregulated in 01-20S. In particular, Bol039180 (an invertase/pectin methylesterase inhibitor family protein) is likely involved in pectin degradation, and might play an important role in the pollen separation defects of 01-20S. This study facilitates a better understanding of DNA methylation alterations and their possible roles in genic male sterility in cabbages.

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Section: Discussionmentioning

confidence: 99%

“…Chen et al observed higher DNA methylation levels in maize fertility-restored hybrids than in maize sterility-maintained hybrids and detected a specific site (16–1) in one of the restorer genes, Rf5 [27]. This finding suggests that DNA methylation may regulate the expression of fertility restorer genes.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Genome Wide DNA Methylation Profiles for the Genic Male Sterile Cabbage Line 01-20S and Its Maintainer Line

Han

Zhang

Liu

et al. 2017

Genes

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“…Emerging evidence has shown that dynamic changes in DNA methylation may mediate transcriptional variation during male reproductive development [35,36]. Over the past several years, the association of DNA methylation status with CMS or genic male sterility (GMS) has been reported in many plants, such as rice [37], wheat [38], maize [39], cotton [35,40], cabbage [41], and tomato [42]. These observations suggest that disrupted genomic DNA methylation in response to high-temperature stress disturbs sugar and ROS metabolism, ultimately resulting in microspore abortion in GMS cotton [35].…”

Section: Introductionmentioning

confidence: 99%

Integrated Methylome and Transcriptome Analysis between the CMS-D2 Line ZBA and Its Maintainer Line ZB in Upland Cotton

Zhang

Guo

et al. 2019

IJMS

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DNA methylation is an important epigenetic modification involved in multiple biological processes. Altered methylation patterns have been reported to be associated with male sterility in some plants, but their role in cotton cytoplasmic male sterility (CMS) remains unclear. Here, integrated methylome and transcriptome analyses were conducted between the CMS-D2 line ZBA and its near-isogenic maintainer line ZB in upland cotton. More methylated cytosine sites (mCs) and higher methylation levels (MLs) were found among the three sequence contexts in ZB compared to ZBA. A total of 4568 differentially methylated regions (DMRs) and 2096 differentially methylated genes (DMGs) were identified. Among the differentially expressed genes (DEGs) associated with DMRs (DMEGs), 396 genes were upregulated and 281 genes were downregulated. A bioinformatics analysis of these DMEGs showed that hyper-DEGs were significantly enriched in the “oxidative phosphorylation” pathway. Further qRT-PCR validation indicated that these hypermethylated genes (encoding the subunits of mitochondrial electron transport chain (ETC) complexes I and V) were all significantly upregulated in ZB. Our biochemical data revealed a higher extent of H2O2 production but a lower level of adenosine triphosphate (ATP) synthesis in CMS-D2 line ZBA. On the basis of the above results, we propose that disrupted DNA methylation in ZBA may disrupt the homeostasis of reactive oxygen species (ROS) production and ATP synthesis in mitochondria, triggering a burst of ROS that is transferred to the nucleus to initiate programmed cell death (PCD) prematurely, ultimately leading to microspore abortion. This study illustrates the important role of DNA methylation in cotton CMS.

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“…Changes in DNA methylation have been shown to play a crucial part in plant stress responses [6]. Additionally, some recent reports revealed that DNA methylation levels are affected by DMTs and regulated the expression of genes involved in cytoplasmic male sterile in rice, maize, and wheat [56][57][58]. However, few studies have been reported about exploring the roles of DMTs in TGMS wheat during the reproductive stage.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide Identification and Transcriptional Characterization of Dna Methyltransferases Conferring Temperature-sensitive Male Sterility in Wheat

Feng

Liu

et al. 2020

Preprint

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Background: DNA methyltransferase (DMT) genes contribute to plant stress responses and development by de novo establishment and subsequent maintenance of DNA methylation during replication. The photoperiod or/and temperature-sensitive genic male sterile (P/TGMS) lines play an important role in hybrid seed production of wheat. However, only few studies have been reported about the effect of the DMT genes on temperature-sensitive male sterility of wheat. Although DMT genes have been researched in some plant species, the identification and analysis of DMT genes in wheat (Triticum aestivum L.) based on genome-wide levels has not been reported.Results: In this study, a detailed overview of phylogeny of 52 wheat DMT (TaDMT) genes was presented. Homoeolog retention for TaDMT genes was significantly above the average retention rate for whole-wheat genes, indicating the functional importance of many DMT homoeologs. We found that the strikingly high number of TaDMT genes mainly resulted from the significant expansion of TaDRM subfamily. Intriguingly, all 5 paralogs belonged to the wheat DRM subfamily and we speculated tandem duplications might play a crucial role in the TaDRM subfamily expansion. Through the transcriptional analysis of TaDMT genes in a TGMS line BS366 and its hybrids and its hybrids with other six fertile lines under sterile and fertile conditions, we concluded that TaCMT-D2, TaMET1-B1, and TaDRM-U6 might be involved in male sterility in BS366. Furthermore, a correlation analysis showed that TaMET1-B1 might negatively regulate the expression of TaRAFTIN1A, an important gene for pollen development, so we speculated an epigenetic regulatory mechanism underling the male sterility of BS366 by the interaction between TaMET1-B1 and TaRAFTIN1A.Conclusions: Our findings presented a detailed phylogenic overview of the DMT genes and could provide novel insights into the effects of the DMT genes on TGMS wheat.

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DNA methylation analysis of sterile and fertile CMS-C hybrids and their parents in maize

Cited by 16 publications

References 23 publications

Comparative Analysis of Genome Wide DNA Methylation Profiles for the Genic Male Sterile Cabbage Line 01-20S and Its Maintainer Line

Comparative Analysis of Genome Wide DNA Methylation Profiles for the Genic Male Sterile Cabbage Line 01-20S and Its Maintainer Line

Integrated Methylome and Transcriptome Analysis between the CMS-D2 Line ZBA and Its Maintainer Line ZB in Upland Cotton

Genome-wide Identification and Transcriptional Characterization of Dna Methyltransferases Conferring Temperature-sensitive Male Sterility in Wheat

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