DNA methylation as a regulator of intestinal gene expression

Pinho, Raquel M.; Maga, Elizabeth A.

doi:10.1017/s0007114521000556

Cited by 12 publications

(8 citation statements)

References 188 publications

(246 reference statements)

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“…For example, the conversion of 5‐mC to 5‐hmC is considered to be an important regulatory mechanism for the expression of Wnt genes. [ 36 ] Consequently, increased levels of Wnt3 mRNA levels were observed in Hydra after photostimulation with P3HT‐NP photostimulation (Figure 1E). Therefore, we postulate that 5‐hmC may play an important role during NP photostimulation‐mediated hormetic response.…”

Section: Resultsmentioning

confidence: 99%

Optical Control of Tissue Regeneration through Photostimulation of Organic Semiconducting Nanoparticles

Onorato

Fardella

Lewińska

et al. 2022

Adv Healthcare Materials

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Next generation bioengineering strives to identify crucial cues that trigger regeneration of damaged tissues, and to control the cells that execute these programs with biomaterials and devices. Molecular and biophysical mechanisms driving embryogenesis may inspire novel tools to reactivate developmental programs in situ. Here nanoparticles based on conjugated polymers are employed for optical control of regenerating tissues by using an animal with unlimited regenerative potential, the polyp Hydra, as in vivo model, and human keratinocytes as an in vitro model to investigate skin repair. By integrating animal, cellular, molecular, and biochemical approaches, nanoparticles based on poly-3-hexylthiophene (P3HT) are shown able to enhance regeneration kinetics, stem cell proliferation, and biomolecule oxidation levels. Opposite outputs are obtained with PCPDTBT-NPs (Poly[2,6-(4,4-bis-(2-ethylhexyl)-4H-cyclopenta [2,1-b;3,4-b′] dithiophene)-alt-4,7(2,1,3-benzothiadiazole)], causing a beneficial effect on Hydra regeneration but not on the migratory capability of keratinocytes. These results suggest that the artificial modulation of the redox potential in injured tissues may represent a powerful modality to control their regenerative potential. Importantly, the possibility to fine-tuning materials' photocatalytic efficiency may enable a biphasic modulation over a wide dynamic range, which can be exploited to augment the tissue regenerative capacity or inhibit the unlimited potential of cancerous cells in pathological contexts.

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Section: Resultsmentioning

confidence: 99%

Optical Control of Tissue Regeneration through Photostimulation of Organic Semiconducting Nanoparticles

Onorato

Fardella

Lewińska

et al. 2022

Adv Healthcare Materials

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“…To determine why the expression of KCNJ14 is abnormally increased in colorectal cancer cells, we downloaded the DNA methylation data of patients with colorectal disease from the TCGA database. Based on the important regulatory effect of DNA methylation on the expression of downstream mRNA [ 17 ], from a large number of DNA methylation sites, we selected 11 CpG sites with regulatory effects on KCNJ14 expression (Additional file 1 : Fig. S1a).…”

Section: Resultsmentioning

confidence: 99%

KCNJ14 knockdown significantly inhibited the proliferation and migration of colorectal cells

Pan

et al. 2022

BMC Med Genomics

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Background This study attempted to verify the potential of KCNJ14 as a biomarker in colorectal cancer (CRC). Methods Data on transcriptomics and DNA methylation and the clinical information of CRC patients were downloaded from The Cancer Genome Atlas and Gene Expression Omnibus databases. Biological information analysis methods were conducted to determine the role of KCNJ14 in the prognosis, diagnosis, immune cell infiltration, and regulation mechanism of CRC patients. The effect of KCNJ14 on the proliferation and migration of HCT116 and SW480 CRC cell lines was verified by in vitro experiments (MTT, colony-forming, wound healing, and transwell assays). Western blotting was performed to detect the effect of KCNJ14 on the levels of mTOR signalling pathway-related proteins. Results KCNJ14 expression was remarkably increased in CRC tissues and cell lines, which reduced the overall survival time of patients. KCNJ14 mRNA was negatively regulated by its methylation site cg17660703, which can also endanger the prognosis of patients with CRC. Functional enrichment analysis suggested that KCNJ14 is involved in the mTOR, NOD-like receptor, and VEGF signalling pathways. KCNJ14 expression was positively correlated with the number of CD4 + T cells and negatively correlated with that of CD8 + T cells in the immune microenvironment. KCNJ14 knockdown significantly reduced not only the proliferation and migration of CRC cell lines but also the levels of mTOR signalling pathway-related proteins. Conclusions This study not only increases the molecular understanding of KCNJ14 but also provides a potentially valuable biological target for the treatment of colorectal cancer.

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“…Many of the genetic mutations associated with IBD are related to immune function, and in particular with interactions between the immune system and the microbiome. The microbiota affects the activation of certain genes associated with hypomethylated active regulatory regions, thus causing the expression of genes associated with IBD [ 26 ]. The susceptibility genes for IBD involved in pathogen recognition and elimination likely influence dysbiosis.…”

Section: Etiology—genetics Factorsmentioning

confidence: 99%

Genetic and Epigenetic Etiology of Inflammatory Bowel Disease: An Update

Jarmakiewicz-Czaja

Zielińska

Sokal

et al. 2022

Genes

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Inflammatory bowel disease (IBD) is a chronic disease with periods of exacerbation and remission of the disease. The etiology of IBD is not fully understood. Many studies point to the presence of genetic, immunological, environmental, and microbiological factors and the interactions between them in the occurrence of IBD. The review looks at genetic factors in the context of both IBD predisposition and pharmacogenetics.

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DNA methylation as a regulator of intestinal gene expression

Cited by 12 publications

References 188 publications

Optical Control of Tissue Regeneration through Photostimulation of Organic Semiconducting Nanoparticles

Optical Control of Tissue Regeneration through Photostimulation of Organic Semiconducting Nanoparticles

KCNJ14 knockdown significantly inhibited the proliferation and migration of colorectal cells

Genetic and Epigenetic Etiology of Inflammatory Bowel Disease: An Update

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