DNA methylation levels and telomere length in human teeth: usefulness for age estimation

Ruiz, Adolfina; González-Herrera, Lucas; Luna, Juan de Dios; Valenzuela, Abel

doi:10.1007/s00414-019-02242-7

Cited by 39 publications

(31 citation statements)

References 39 publications

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“…In contrast to other studies, results with EDARADD and MIR29B2C using Sanger sequencing showed no significant correlation with age, while ELOVL2, FHL2, and PDE4C showed weak correlations. However, their ELOVL2 correlations were similar to the ones obtained in the present study and previous works [ 25 , 30 ]. In addition, the proposed model was a simple linear regression, providing estimates with FHL2 CpG4 with a MAD of 11.35 years.…”

Section: Discussionsupporting

confidence: 93%

“…The objective of this study was to improve age-at-death estimates in forensic anthropology, based on epigenetics, particularly, the assessment of DNA methylation. This was supported by previous work applied to this discipline [ 25 , 29 , 30 , 31 ]. Teeth are the hardest tissues in human body, thus the present study focused on pulp, as it is the inner layer of the teeth, well protected from environmental insult.…”

Section: Discussionsupporting

confidence: 79%

“…Unlike previous studies that have demonstrated a difference in age estimation among tooth layers, more recent work examining teeth utilized the DNA methylation levels of the whole tooth for age correlations. The study of Márquez-Ruiz et al [ 30 ] developed multivariate models based on three genes (ELOVL2, ASPA, and EDARADD). As in the present study, certain CpGs that were less significant but still correlated with age were included in the models, improving the estimates, and providing a MAE between 4.80 and 5.08 years.…”

Section: Discussionmentioning

confidence: 99%

“…Giuliani et al [ 29 ] analyzed the methylation status of three genes in different dental tissues (cementum, dentin, and pulp) obtaining accuracies between 1.2 and 7.1 years, by applying EpiTYPER, a MALDI-TOF mass spectrometry-based method. Recently, Márquez-Ruiz et al [ 30 ] also applied pyrosequencing to determine the age in teeth based on three genes, achieving accuracies between 4.8 and 6.9 years. Correia-Dias et al [ 31 ] applied two methodologies for methylation analysis—Sanger sequencing and SNaPshot—obtaining accuracies of 2.6 in bones and 11.4 years in teeth with the former, and 7.2 years in bones and 7.1 years in teeth with the latter.…”

Section: Introductionmentioning

confidence: 99%

“…Correia-Dias et al [ 31 ] applied two methodologies for methylation analysis—Sanger sequencing and SNaPshot—obtaining accuracies of 2.6 in bones and 11.4 years in teeth with the former, and 7.2 years in bones and 7.1 years in teeth with the latter. These last two studies [ 30 , 31 ] analyzed methylation on the whole tooth, not distinguishing between tissues. Recent work comparing methylation differences between types of dental tissues [ 29 ] indicate that isolating specific dental structures, such as pulp, might improve accuracy.…”

Section: Introductionmentioning

confidence: 99%

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Identifying Methylation Patterns in Dental Pulp Aging: Application to Age-at-Death Estimation in Forensic Anthropology

Zapico

Gauthier

Antevska

et al. 2021

IJMS

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Age-at-death estimation constitutes one of the key parameters for identification of human remains in forensic investigations. However, for applications in forensic anthropology, many current methods are not sufficiently accurate for adult individuals, leading to chronological age estimates erring by ±10 years. Based on recent trends in aging studies, DNA methylation has great potential as a solution to this problem. However, there are only a few studies that have been published utilizing DNA methylation to determine age from human remains. The aim of the present study was to expand the range of this work by analyzing DNA methylation in dental pulp from adult individuals. Healthy erupted third molars were extracted from individuals aged 22–70. DNA from pulp was isolated and bisulfite converted. Pyrosequencing was the chosen technique to assess DNA methylation. As noted in previous studies, we found that ELOVL2 and FHL2 CpGs played a role in age estimation. In addition, three new markers were evaluated—NPTX2, KLF14, and SCGN. A set of CpGs from these five loci was used in four different multivariate regression models, providing a Mean Absolute Error (MAE) between predicted and chronological age of 1.5–2.13 years. The findings from this research can improve age estimation, increasing the accuracy of identification in forensic anthropology.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Identifying Methylation Patterns in Dental Pulp Aging: Application to Age-at-Death Estimation in Forensic Anthropology

Zapico

Gauthier

Antevska

et al. 2021

IJMS

View full text Add to dashboard Cite

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Genome‐wide identification of age‐related CpG sites for age estimation from blood DNA of Han Chinese individuals

Xiao

Huang

2021

Electrophoresis

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Age‐related CpG (AR‐CpG) sites are currently the most promising molecular markers for forensic age estimation. However, the AR‐CpG sites of Han Chinese population remains to be systematically characterized. In this study, we performed genome‐wide methylation analyses on 42 whole blood DNA from healthy Han Chinese volunteers (aged from 18 to 62 years) using the Illumina MethylationEPIC BeadChip microarray. As expected, both known and novel AR‐CpG sites were identified. Considering the sex difference in aging rate, we then separately selected AR‐CpG candidates and built pyrosequencing‐based multiple linear regression models for age estimation of males and females. The model constructed from the male sample group (n = 167, aged from 1.50 to 85.71 years) explained 95.22% of variation in age using five AR‐CpG sites (chr6:11044864 ELOVL2, chr1:207997068 C1orf132, cg19283806 CCDC102B, cg17740900, and chr10:73740306 CHST3) and yielded a mean absolute error (MAE) of 2.79 years. The model constructed from the female sample group (n = 141, aged from 3.33 to 80.38 years) explained 94.90% of variation in age with six AR‐CpG sites (chr6:11044867 ELOVL2, chr1:207997060 C1orf132, chr2:106015757 FHL2, cg26947034, chr16: 67184108 B3GNT9, and chr20:44658203 SLC12A5) and yielded an MAE of 2.53 years. Besides, the estimated age was highly correlated with the actual age (R > 0.97). The robustness of these AR‐CpG markers was demonstrated by 10‐fold cross‐validations. In conclusion, we updated the AR‐CpG sites of Han Chinese population and provided two sets of AR‐CpG sites for accurate age estimation.

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Pyrosequencing: Current forensic methodology and future applications—a review

et al. 2022

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The recent development of small, single-amplicon-based benchtop systems for pyrosequencing has opened up a host of novel procedures for applications in forensic science. Pyrosequencing is a sequencing by synthesis technique, based on chemiluminescent inorganic pyrophosphate detection. This review explains the pyrosequencing workflow and illustrates the step-by-step chemistry, followed by a description of the assay design and factors to keep in mind for an exemplary assay. Existing and potential forensic applications are highlighted using this technology. Current applications include identifying species, identifying bodily fluids, and determining smoking status. We also review progress in potential applications for the future, including research on distinguishing monozygotic twins, detecting alcohol and drug abuse, and other phenotypic characteristics such as diet and body mass index. Overall, the versatility of the pyrosequencing technologies renders it a useful tool in forensic genomics.

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DNA methylation levels and telomere length in human teeth: usefulness for age estimation

Cited by 39 publications

References 39 publications

Identifying Methylation Patterns in Dental Pulp Aging: Application to Age-at-Death Estimation in Forensic Anthropology

Identifying Methylation Patterns in Dental Pulp Aging: Application to Age-at-Death Estimation in Forensic Anthropology

Genome‐wide identification of age‐related CpG sites for age estimation from blood DNA of Han Chinese individuals

Pyrosequencing: Current forensic methodology and future applications—a review

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