DNA methylation of dermal MSCs in psoriasis: Identification of epigenetically dysregulated genes

Hou, Ruixia; Yin, Guohua; An, Peng; Wang, Chunfang; Liu, Ruifeng; Yang, Yaru; Yan, Xin; Li, Junqin; Li, Xinhua; Zhang, Kaiming

doi:10.1016/j.jdermsci.2013.07.002

Cited by 54 publications

(53 citation statements)

References 37 publications

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“…DMSCs were isolated and expanded as previously described (Hou et al, 2013). Briefly, the dermis was separated from the epidermis using 0.25% dispase (Sigma-Aldrich, St. Louis, MO, USA) and subsequently digested into a monoplast suspension.…”

Section: Isolation and Expansion Of Dmscsmentioning

confidence: 99%

“…Moreover, differential gene expression analysis showed that the expression of genes related to immune responses, inflammation, and lymphocyte adhesion and chemotaxis in MSCs were down-regulated in psoriatic skin lesions Hou et al, 2014). Based on previous studies, we applied gene chip technology to conduct a comparative analysis of gene expression patterns in dermal MSCs (DMSCs) in skin lesions of psoriasis patients and normal skin of healthy individuals (Hou et al, 2013(Hou et al, , 2014. Abnormalities were detected in the methylation status and expression level of a number of genes related to inflammation, immune responses, angiogenesis, and cell proliferation (Hou et al, 2013(Hou et al, , 2014.…”

Section: Introductionmentioning

confidence: 99%

“…Based on previous studies, we applied gene chip technology to conduct a comparative analysis of gene expression patterns in dermal MSCs (DMSCs) in skin lesions of psoriasis patients and normal skin of healthy individuals (Hou et al, 2013(Hou et al, , 2014. Abnormalities were detected in the methylation status and expression level of a number of genes related to inflammation, immune responses, angiogenesis, and cell proliferation (Hou et al, 2013(Hou et al, , 2014. Our previous findings suggested that MSCs in skin lesions may be involved in localized immune responses and proliferative expansion of blood vessels in psoriasis.…”

Section: Introductionmentioning

confidence: 99%

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LITAF, HHEX, and DUSP1 expression in mesenchymal stem cells from patients with psoriasis

Chang¹,

Niu²,

Hou³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Psoriasis is a common chronic relapsing inflammatory skin disease, in which mesenchymal stem cells (MSCs) have been hypothesized to play an important role in abnormal localized inflammation and vascular proliferation observed in skin lesions. Previous studies have revealed abnormal gene expression patterns, DNA methylation status, and cytokine secretion of MSCs in psoriatic skin lesions, as well as some gene expression abnormalities related to inflammation and angiogenesis. We further verified the gene and protein expressions of inflammation-related lipopolysaccharide-induced tumor necrosis factor-alpha transcription factor (LITAF), dual-specificity protein phosphatase 1 (DUSP1), and angiogenesis-related hematopoietically expressed homeobox (HHEX) in MSCs derived from the skin lesions of psoriasis patients. The gene expression of LITAF, DUSP1, and HHEX in dermal MSCs was measured at the mRNA level using reverse transcription-polymerase chain reaction and the corresponding protein expression levels were analyzed by western (2015) blotting analysis. The gene and protein expression levels of LITAF, HHEX, and DUSP1 in dermal MSCs were significantly lower in psoriasis patients compared to controls. Amplification and western blotting results were consistent with our previously reported gene chip data. Our results suggest that dermal MSCs in psoriatic skin lesions may be involved in the development, progression, and regulation of localized inflammatory abnormalities by reducing the expression of LITAF, HHEX, and DUSP1, which are related to inflammation and angiogenesis.

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Section: Isolation and Expansion Of Dmscsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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LITAF, HHEX, and DUSP1 expression in mesenchymal stem cells from patients with psoriasis

Chang¹,

Niu²,

Hou³

et al. 2015

Genet. Mol. Res.

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“…Los efectos de la metilación del ADN y las modificaciones de las histonas en psoriasis han sido objeto de excelentes revisiones [25][26][27] y no serán abordados aquí. En esta revisión nos centraremos en los nc-ARN, específicamente en los mi-ARN y su papel en la psoriasis con compromiso cutáneo.…”

Section: Importancia De La Regulación Epigenética En La Psoriasisunclassified

Aspectos inmunogenéticos de la psoriasis con énfasis en micro-ARN

Cañas

Lopera

Barrera³

2014

Inmunología

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“…In preliminary studies, several abnormal biological features have been found in bone marrow and skin lesion mesenchymal stem cells (MSCs) of psoriasis patients (Zhang et al, 2010;Hou et al, 2013Hou et al, , 2014Liu et al, 2013Liu et al, , 2014. Skin MSCs (S-MSCs) are an important component of the microenvironment of the skin.…”

Section: Introductionmentioning

confidence: 99%

Research Note Mesenchymal stem cells from skin lesions of psoriasis patients promote proliferation and inhibit apoptosis of HaCaT cells

Liu¹,

Wang²,

Wang³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Psoriasis is an inflammatory skin disease characterized by excessive proliferation and abnormal differentiation and apoptosis of keratinocytes (KCs). Mesenchymal stem cells (MSCs) from skin lesions of psoriasis patients demonstrate abnormal cytokine secretion, which may affect KC proliferation and apoptosis. Here, we explored how MSCs from skin lesions of psoriasis patients affect HaCaT cell proliferation and apoptosis. First, flow cytometry and multipotent differentiation methods were used to identify skin MSCs, which were then co-cultured with HaCaT cells. HaCaT cell proliferation was analyzed in real-time, and cell cycle progression and apoptosis were assessed by flow cytometry. Cell morphologies and multipotencies of skin MSCs were similar between the psoriasis group and healthy control group, with high levels of CD29, CD44, CD73, CD90, and CD105 and limited expression of CD34, CD45, and HLA-DR. MSCs from skin lesions of psoriasis patients promote KC proliferation more potently and are less capable of inducing KC apoptosis. This may Research Note 17759MSC in psoriatic skin lesions ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 17758-17767 (2015) underlie KC proliferation and abnormal apoptosis in psoriasis skin lesions, which results in abnormal thickening of the epidermis.

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DNA methylation of dermal MSCs in psoriasis: Identification of epigenetically dysregulated genes

Cited by 54 publications

References 37 publications

LITAF, HHEX, and DUSP1 expression in mesenchymal stem cells from patients with psoriasis

LITAF, HHEX, and DUSP1 expression in mesenchymal stem cells from patients with psoriasis

Aspectos inmunogenéticos de la psoriasis con énfasis en micro-ARN

Research Note Mesenchymal stem cells from skin lesions of psoriasis patients promote proliferation and inhibit apoptosis of HaCaT cells

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