DNA methylation of multiple genes and clinicopathological relationship of non-small cell lung cancers

Hanabata, Tetsuro; Tsukuda, Kazunori; Toyooka, Shinichi; Yano, Masaaki; Aoe, Motoi; Nagahiro, Itaru; Sano, Yoshifumi; Date, Hiroshi; Shimizu, Nobuyoshi

doi:10.3892/or.12.1.177

Cited by 16 publications

(17 citation statements)

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“…However, the ability of Dnmt proteins generated in a cytosolic in vitro translation system to bind to bacterially expressed LANA suggests that the Dnmt proteins can contact LANA directly. The LANA-repressed gene CDH13 is down-regulated through methylation in breast cancer, non-small cell lung cancer, colorectal cancer, invasive squamous cell carcinoma, and chronic myeloid leukemia (38,(51)(52)(53)(54). Promoter methylation and loss of expression of lactate dehydrogenase B has been described in prostate and gastric cancers (55,56), and promoter hypermethylation of the CCND2 gene occurs in several cancers including prostate (57), acute lymphoblastic leukemia (58), and breast (59).…”

Section: Discussionmentioning

confidence: 99%

Recruitment of the de novo DNA methyltransferase Dnmt3a by Kaposi's sarcoma-associated herpesvirus LANA

Shamay

Krithivas

Zhang

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

157

143

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The Kaposi's sarcoma-associated herpesvirus LANA protein is expressed in all Kaposi's sarcoma-associated herpesvirus-infected cells, including the tumor cells of endemic and AIDS-associated Kaposi sarcoma, primary effusion lymphoma, and Castleman disease. LANA modulates cell gene expression, but the mechanisms of LANA-mediated transcriptional reprogramming are poorly understood. LANA-repressed cell genes were identified by using retroviral-transduced telomerase-immortalized microvascular endothelial cells. Transciptional repression of targeted genes was relieved by treatment with the methyltransferase inhibitor 5-aza-2-deoxycytidine, suggesting a role for DNA methylation in repression. We found that LANA coprecipitated with DNA methyltransferases (Dnmts) and recruited endogenous DNA methyltransferase activity from the cell extract. LANA preferentially relocalized Dnmt3a from the nuclear matrix into the chromatin fraction. Further, LANA associated with repressed cellular promoters, recruited Dnmt3a to DNA, and facilitated de novo promoter methylation of a downregulated gene, cadherin 13 (H-cadherin). The data provide an example of promoter-specific epigenetic DNA modification through viral protein recruitment of de novo Dnmt activity.cancer ͉ CpG methylation ͉ epigenetic modification ͉ transcription repression

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Section: Discussionmentioning

confidence: 99%

Recruitment of the de novo DNA methyltransferase Dnmt3a by Kaposi's sarcoma-associated herpesvirus LANA

Shamay

Krithivas

Zhang

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

157

143

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“…Moreover, H-cadherin methylation occurred more frequently in adenocarcinoma (49%) than in squamous cell carcinoma (35%; P ϭ 0.03), consistent with previous findings. 3,15 No significant correlation was found between H-cadherin methylation and recurrence or differentiation.…”

Section: Clinicopathologic Characteristicsmentioning

confidence: 96%

“…9,10,12,13 Aberrant methylation of CpG islands within the promoters of tumor suppressor genes is one of the most frequently acquired epigenetic changes during lung carcinoma carcinogenesis. [13][14][15][16] In the current study, we investigated the aberrant methylation of H-cadherin promoter in 305 primary NSCLCs to assess the clinicopathologic and prognostic significance of H-cadherin methylation.…”

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confidence: 99%

Aberrant methylation of H‐cadherin (CDH13) promoter is associated with tumor progression in primary nonsmall cell lung carcinoma

Kim

Han

Shim

et al. 2005

Cancer

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Morphometrics of the molar crown is based traditionally on diameter measurements but is nowadays more often based on 2D image analysis of crown outlines. An alternative approach involves measurements at the level of the cervical line. We compare the information content of the two options in a three‐dimensional (3D) digital sample of lower and upper first molars (M1 and M1) of modern human and Neanderthal teeth. The cervical outline for each tooth was created by digitizing the cervical line and then sectioning the tooth with a best fit plane. The crown outline was projected onto this same plane. The curves were analyzed by direct extraction of diameters, diagonals, and area and also by principal component analysis either of the residuals obtained by regressing out these measurements from the radii (shape information) or directly by the radii (size and shape information). For M1, the crown and cervical outline radii allow us to discriminate between Neanderthals and modern humans with 90% and 95% accuracy, respectively. Fairly good discrimination between the groups (80–82.5%) was also obtained using cervical measurements. With respect to M1, general overlap of the two groups was obtained by both crown and cervical measurements; however, the two taxa were differentiable by crown outline residuals (90–97%). Accordingly, while crown diameters or crown radii should be used for taxonomic analysis of unworn or slightly worn M1s, the crown outline, after regressing out size information, could be promising for taxonomic assignment of lower M1s. Am J Phys Anthropol, 2011. © 2010 Wiley‐Liss, Inc.

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“…For example, several studies analyzed CDKN2A methylation using the same primers. However, different methylation frequencies were detected because different annealing temperatures and master mixes were used in these studies (10,12,13,32). Because of the incorporation of sequence-specific probes in quantitative MSP assays, these assays tend to have improved specificities due to elimination of nonspecific products detection and identification of only fully methylated sequences.…”

Section: Promoter Hypermethylation and Lung Cancer Cancer Epidemiol Bmentioning

confidence: 99%

“…Multiple studies have assessed the epigenetic changes present in NSCLC (4,5), reporting a wide range of hypermethylation frequencies. Some studies have compared the methylation changes of NSCLC with that of matching noncancerous lung tissue from the same patient (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17). Most of these studies relied on a qualitative methylation-specific PCR (MSP) methodology that is subjective, relying on the detection of a band after electrophoresis, and does not distinguish between low-level and high-level methylation.…”

Section: Introductionmentioning

confidence: 99%

DNA Methylation in Tumor and Matched Normal Tissues from Non-Small Cell Lung Cancer Patients

Feng¹,

Hawes

Stern³

et al. 2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

150

115

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We used MethyLight assays to analyze DNA methylation status of 27 genes on 49 paired cancerous and noncancerous tissue samples from non-small cell lung cancer (NSCLC) patients who underwent surgical resection. Seven genes (RARB, BVES, CDKN2A, KCNH5, RASSF1, CDH13, and RUNX) were found to be methylated significantly more frequently in tumor tissues than in noncancerous tissues. Only methylation of CCND2 and APC was frequently detected in both cancerous and noncancerous tissues, supporting the hypothesis that the methylation of these two genes is a preneoplastic change and may be associated with tobacco smoking exposure. Methylation of any one of eight genes (RASSF1, DAPK1, BVES, CDH13, MGMT, KCNH5, RARB, or CDH1) was present in 80% of NSCLC tissues but only in 14% of noncancerous tissues. Detection of methylation of these genes in blood might have utility in monitoring and detecting tumor recurrence in earlystage NSCLC after curative surgical resection. (Cancer Epidemiol Biomarkers Prev 2008;17(3):645 -54)

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DNA methylation of multiple genes and clinicopathological relationship of non-small cell lung cancers

Cited by 16 publications

References 0 publications

Recruitment of the de novo DNA methyltransferase Dnmt3a by Kaposi's sarcoma-associated herpesvirus LANA

Recruitment of the de novo DNA methyltransferase Dnmt3a by Kaposi's sarcoma-associated herpesvirus LANA

Aberrant methylation of H‐cadherin (CDH13) promoter is associated with tumor progression in primary nonsmall cell lung carcinoma

DNA Methylation in Tumor and Matched Normal Tissues from Non-Small Cell Lung Cancer Patients

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