DNA methylation of the RE-1 silencing transcription factor in peripheral blood mononuclear cells and gene expression of antioxidant enzyme in patients with late-onset Alzheimer disease

González-Mundo, Ilicia; Pérez-Vielma, Nadia Mabel; Gómez-López, Modesto; Fleury, Agnès; Correa‐Basurto, José; Rosales‐Hernández, Martha Cecilia; Sixto-López, Yudibeth; Martínez-Godínez, María de los Ángeles; Domínguez‐López, Aarón; Miliar-García, Ángel

doi:10.1016/j.exger.2020.110951

Cited by 10 publications

(8 citation statements)

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“…In another study conducted in late-onset AD (LOAD) patients, it was observed that CAT gene expression levels in peripheral blood were significantly decreased compared to the controls, but the erythrocyte CAT enzymatic activity was significantly increased in LOAD patients. This suggests that even as the expression of antioxidant genes decreased, their enzymatic activity was not affected (24). Consistent with that study, we observed a decreased mRNA expression of CAT in AD patients but without statistical significance.…”

Section: Discussionsupporting

confidence: 90%

Peripheral Expression Levels of Selected Oxidative Stress-Related Genes in Alzheimer’s Disease

Erginel-Unaltuna¹

2021

experimed

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Objective: The etiology of Alzheimer's disease (AD) is affected via oxidative stress. Antioxidant enzymes are extremely important in preventing reactive oxygen species (ROS) causing damage in the cell. The changes in expression levels of oxidant and antioxidant genes are key factors in cell response to oxidative stress. As a result, this study investigated the change in expression levels of specific oxidative stress related genes (solute carrier family 7 member 11 (SLC7A11), glutathione peroxidase 4 (GPX4), catalase (CAT) and acylcoa synthetase long chain family member 4 (ACSL4)) in peripheral blood of AD patients. Materials and Methods:Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was used to assess the expression levels of oxidative stress-related genes in 25 AD patients and 22 controls, and the findings were statistically evaluated.Results: SLC7A11, GPX4, CAT, and ACSL4 gene expression levels did not vary significantly between AD patients and controls. The results also showed significant negative correlation between age of onset and ACSL4 expression. Conclusion:This is the first study that evaluated mRNA expression levels of SLC7A11, GPX4 and ACSL4 genes in AD. The results suggested that the peripheral blood expression of above-mentioned genes did not alter in AD. However, due to the small number of subjects, this findings are preliminary and should be validated with a larger number of subjects.

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Section: Discussionsupporting

confidence: 90%

Peripheral Expression Levels of Selected Oxidative Stress-Related Genes in Alzheimer’s Disease

Erginel-Unaltuna¹

2021

experimed

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“…Its minimal promoter region was highly methylated, and a significant reduction in antioxidant enzyme transcription was found. It was demonstrated that REST methylation may induce alterations in AD pathophysiology, which may cause the production of ROS [ 36 ].…”

Section: Zinc and Agingmentioning

confidence: 99%

Zinc in Cognitive Impairment and Aging

et al. 2022

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Zinc, an essential micronutrient for life, was first discovered in 1869 and later found to be indispensable for the normal development of plants and for the normal growth of rats and birds. Zinc plays an important role in many physiological and pathological processes in normal mammalian brain development, especially in the development of the central nervous system. Zinc deficiency can lead to neurodegenerative diseases, mental abnormalities, sleep disorders, tumors, vascular diseases, and other pathological conditions, which can cause cognitive impairment and premature aging. This study aimed to review the important effects of zinc and zinc-associated proteins in cognitive impairment and aging, to reveal its molecular mechanism, and to highlight potential interventions for zinc-associated aging and cognitive impairments.

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“…High oxidative stress was also observed in blood leukocytes isolated from an AD animal model (Robinson, Cao, & Williams, 2013). Interestingly, in both humans and animals, increased expression of toxic product‐generating enzymes such as nitric oxide synthase (NOS) and xanthine oxidase (De Servi, La Porta, Bontempelli, & Comolli, 2002; Maté, Cruces, Giménez‐Llort, & De la Fuente, 2015), as well as altered levels of free radical scavengers and anti‐oxidant enzymes like reduced glutathione, superoxide dismutase (SOD), catalase, glutathione peroxidases, thioredoxin reductase, and heme oxygenase‐1 were reported in peripheral AD leukocytes (Calabrese et al., 2006; De Leo et al., 1998; Gatta et al., 2009; González‐Mundo et al., 2020; Ishizuka et al., 2002; Maté et al., 2015). Some studies also showed changes in the expression levels and/or activity of complexes and subunits of the mitochondrial respiratory chain in leukocytes from AD patients, including complex II and IV, and ATP synthase B, but results are not consistent between studies (Feldhaus et al., 2011; Sultana et al., 2013; Valla et al., 2006).…”

Section: Metabolic Control Of Immune Cell Activity In Neurological Diseasementioning

confidence: 99%

Metabolic determinants of leukocyte pathogenicity in neurological diseases

Runtsch

Ferrara

Angiari

2020

Journal of Neurochemistry

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DNA methylation of the RE-1 silencing transcription factor in peripheral blood mononuclear cells and gene expression of antioxidant enzyme in patients with late-onset Alzheimer disease

Cited by 10 publications

References 53 publications

Peripheral Expression Levels of Selected Oxidative Stress-Related Genes in Alzheimer’s Disease

Peripheral Expression Levels of Selected Oxidative Stress-Related Genes in Alzheimer’s Disease

Zinc in Cognitive Impairment and Aging

Metabolic determinants of leukocyte pathogenicity in neurological diseases

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