DNA methylation profiles of genes associated with angiogenesis in the samples of placenta in pregnancies complicated by intrauterine growth restriction

Cebe, Fatma Selcen; Tola, Esra Nur; Koşar, Pınar Aslan; Oral, Baha

doi:10.1080/14767058.2019.1671344

Cited by 6 publications

(2 citation statements)

References 31 publications

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“…Several investigations have focused on the DNA methylation in placentas of sFGR, identifying that the growth restricted fetus shows a hypomethylation pattern in placentas compared to the growth normally fetus, and also reporting some differential methylation regions and key regulation pathways 10,11 . According to previous DNA methylation studies, the characterizations of placental DNA methylation have been specifically focused on imprinted genes, growth‐related genes and angiogenesis‐related genes, indicating their potential significance in FGR 12–14 . A recent study has revealed the presence of common genes exhibiting differential methylation in both intrauterine growth restriction and preeclampsia, which suggested the genetic similarity between the two conditions 15 .…”

Section: Introductionmentioning

confidence: 99%

“…10,11 According to previous DNA methylation studies, the characterizations of placental DNA methylation have been specifically focused on imprinted genes, growth-related genes and angiogenesisrelated genes, indicating their potential significance in FGR. [12][13][14] A recent study has revealed the presence of common genes exhibiting differential methylation in both intrauterine growth restriction and preeclampsia, which suggested the genetic similarity between the two conditions. 15 The patterns of DNA methylation in human placenta have been demonstrated to be reliable and significantly associated with fetal growth, which could function as a potential marker for the intrauterine environment and fetal development.…”

Section: Introductionmentioning

confidence: 99%

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Placental DNA methylation analysis of selective fetal growth restriction in monochorionic twins reveals aberrant methylated CYP11A1 gene for fetal growth restriction

Shi,

Zhou,

Cai

et al. 2023

The FASEB Journal

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Fetal growth restriction (FGR) is associated with increased susceptibility to perinatal morbidity and mortality. Evidence suggests that epigenetic changes play critical roles in the regulation of fetal growth. We sought to present a comprehensive analysis of the associations between placental DNA methylation and selective fetal growth restriction (sFGR), which is a severe complication of monochorionic twin pregnancies, characterized by one fetus experiencing restricted growth. Genome‐wide methylation analysis was performed on 24 placental samples obtained from 12 monochorionic twins with sFGR (Cohort 1) using Illumina Infinium MethylationEPIC BeadChip. Integrative analysis of our EPIC data and two previous placental methylation studies of sFGR (a total of 30 placental samples from 15 sFGR twins) was used to identify convincing differential promoter methylation. Validation analysis was performed on the placentas from 15 sFGR twins (30 placental samples), 15 FGR singletons, and 14 control singletons (Cohort 2) using pyrosequencing, quantitative real‐time polymerase chain reaction, western blot, and immunohistochemistry (IHC). A globe shift toward hypomethylation was identified in the placentas of growth‐restricted fetuses compared with the placentas of normal fetuses in monochorionic twins, including 5625 hypomethylated CpGs and 452 hypermethylated CpGs, especially in the regions of CpG islands, gene‐body and promoters. The analysis of pathways revealed dysregulation primarily in steroid hormone biosynthesis, metabolism, cell adhesion, signaling transduction, and immune response. Integrative analysis revealed a differentially methylated promoter region in the CYP11A1 gene, encoding a rate‐limiting enzyme of steroidogenesis converting cholesterol to pregnenolone. The CYP11A1 gene was validated to have hypomethylation and higher mRNA expression in sFGR twins and FGR singletons. In conclusion, our findings suggested that the changes in placental DNA methylation pattern in sFGR may have functional implications for differentially methylated genes and regulatory regions. The study provides reliable evidence for identifying abnormally methylated CYP11A1 gene in the placenta of sFGR.

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Section: Introductionmentioning

confidence: 99%