DNA methylation-regulated microRNA pathways in ovarian serous cystadenocarcinoma: A meta-analysis

Agustriawan, David; Huang, Chien-Hung; Sheu, Jim Jinn‐Chyuan; Lee, Shan-Chih; Tsai, Jeffrey J. P.; Kurubanjerdjit, Nilubon; Ng, Ka-Lok

doi:10.1016/j.compbiolchem.2016.09.016

Cited by 12 publications

(6 citation statements)

References 56 publications

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“…Osaily [ 39 ] reported that miRNA can regulate the methylation of genes associated with breast cancer stem cells. Interestingly, a meta-analysis [ 40 ] indicated that the miR-200 family can cause NDN methylation, which is consistent with our results, and suggested that further exploration of the changes in NDN due to miR-200c is necessary.…”

Section: Discussionsupporting

confidence: 91%

Necdin, one of the important pathway proteins in the regulation of osteosarcoma progression by microRNA-200c

Wang

et al. 2022

Bioengineered

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MicroRNA-200c (miR-200c) generally acts as a tumor suppressor in multiple cancer types and a promising therapeutic target in tumorigenesis. However, only a few studies have explained the role of miR-200c in the development of osteosarcoma (OS). In this study, we investigated the role of miR-200c in OS progression and identified the regulatory pathway protein NDN involved in inhibiting the occurrence and development of OS. Firstly, we found that miR-200c is downregulated in OS cells and tissues. As well, in vitro and in vivo experiments showed that upregulating miR-200c inhibits the proliferation, invasion, metastasis of Saos-2 cells, promotes the apoptosis of Saos-2 cells and suppresses tumor growth in mice, indicating miR-200c plays a major role in regulating the OS progression. Furthermore, bioinformatics analysis showed that an anti-tumor protein, necdin (NDN), might be a potential target by miR-200c. To verify this hypothesis, we measured the expression level of NDN in OS cells and tissues and found NDN is downregulated, suggesting NDN is functional in OS progression. Moreover, we found that the expression levels of NDN and miR-200c in in vivo and in vitro experiments were positively correlated. However, the results of dual-luciferase reporter gene experiment showed miR-200c does not directly act on the 3ʹ untranslated region (UTR) of NDN gene, indicating that NDN might be an important pathway protein which regulates OS progression in the presence of miR-200c. Therefore, miR-200c/NDN could be potential targets for developing effective treatment against OS.

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Section: Discussionsupporting

confidence: 91%

Necdin, one of the important pathway proteins in the regulation of osteosarcoma progression by microRNA-200c

Wang

et al. 2022

Bioengineered

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“…We have demonstrated that DNA methylation of the upstream miRNA promoter region associates with miR-200c expression in OC tissues and partly in plasma. Similarly, a recent meta-analysis on the TCGA cohort revealed a correlation between promoter methylation and miR-200 expression in more than 500 OC patients 24 . While most studies describe the miR-200 family as tumor suppressive miRNAs, downregulated in cancer, some studies have had similar findings to ours.…”

Section: Discussionmentioning

confidence: 84%

“…Since the bulk of circulating cfDNA is likely not tumor specific, we sought to look for a more specific parameter that could be used in combination with cf-miRNAs as a surrogate marker for OC. We hypothesized that the miR-200 family might be epigenetically regulated in OC, as concluded by a recent meta-analysis 24 . The miR-200 family consists of five miRNAs, miR-200a, miR-200b and miR-429; transcribed on chromosome 1 and miR-200c and miR-141; transcribed on chromosome 12.…”

Section: Total Levels Of Circulating Cfdna and Cf-mirnas Are Globally...mentioning

confidence: 95%

Circulating cf-miRNA as a more appropriate surrogate liquid biopsy marker than cfDNA for ovarian cancer

Gahlawat

Witte

Sinn

et al. 2023

Sci Rep

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Ovarian cancer (OC) is an aggressive disease, primarily diagnosed in late stages with only 20% of patients surviving more than 5 years. Liquid biopsy markers have great potential to improve current diagnostic and prognostic methods. Here, we compared miRNAs and DNA methylation in matched plasma, whole blood and tissues as a surrogate marker for OC. We found that while both cfDNA and cf-miRNAs levels were upregulated in OC compared to patients with benign lesions or healthy controls, only cf-miRNA levels were an independent prognosticator of survival. Following on our previous work, we found members of the miR-200 family, miR-200c and miR-141 to be upregulated in both plasma and matched tissues of OC patients which correlated with adverse clinical features. We could also show that the upregulation of miR-200c and -141 correlated with promoter DNA hypomethylation in tissues, but not in plasma or matched whole blood samples. As cf-miRNAs are more easily obtained and very stable in blood, we conclude that they might serve as a more appropriate surrogate liquid biopsy marker than cfDNA for OC.

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“…We identified miRNA-IQGAP-related genes significantly inversely correlated where Spearman rank correlation coefficient <−0.20 and p<0.05. The cutoff is based on our previous study [23]. The results are described in Tables 2-4.…”

Section: Resultsmentioning

confidence: 99%

In Silico Study of Mirna-Regulated Iq Motif-Containing Gtpase-Activating Protein Family in Liver Cancer

Agustriawan

Sumarpo

Parikesit

et al. 2018

Asian J Pharm Clin Res

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Objective: The aim of this paper is to identify the list of microRNA (miRNA) which can regulate the aberrant expression of IQGAP in liver cancer formation. The aberrant expression of IQ motif-containing GTPase-activating protein (IQGAP) family which consists of IQGAP1, IQGAP2, and IQGAP3 has been linked to carcinogenesis in human cancers. The reciprocal expression of IQGAP family in human cancer has been studied to act as oncogenes or tumor suppressor genes. A growing number of studies suggest that upregulated or downregulated expression of IQGAP family triggers cancer development.Methods: A correlation study was performed to construct a pathway to inhibit or activate IQGAP family between miRNAs and IQGAPs. A pre-processing step was conducted to download, filter and process the dataset from TCGA. It yields miRNA and IQGAP gene expression matrix. Then, correlation computation was computed using MATLAB. Moreover, this study linked the results to the MiRTarBase to validate the prediction result with the wet lab experimental result.Results: This study identified significantly inversely correlation in 51 miRNAs-IQGAP1, 169 miRNAs-IQGAP2, and 33 miRNAs-IQGAP3, respectively, which may potentially play a role in a liver cancer formation. Some of the results also can be found in miRTarBase. It supports the precision of those miRNA and IQGAP interaction between dry lab and wet lab study. IQGAP1 and IQGAP2 mostly has been identified as an oncogene in cancer but IQGAP2 has been discovered as tumor suppressor gene. The list of miRNA in the result of this study can become a potential therapy to target the aberrant expression of IQGAP family.Conclusion: miRNA function is known as an oncogene or tumor suppressor gene in cancer development. Therefore, it can be one of the important molecular biology which may target the aberrant expression of IQGAP in liver cancer.

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DNA methylation-regulated microRNA pathways in ovarian serous cystadenocarcinoma: A meta-analysis

Cited by 12 publications

References 56 publications

Necdin, one of the important pathway proteins in the regulation of osteosarcoma progression by microRNA-200c

Necdin, one of the important pathway proteins in the regulation of osteosarcoma progression by microRNA-200c

Circulating cf-miRNA as a more appropriate surrogate liquid biopsy marker than cfDNA for ovarian cancer

In Silico Study of Mirna-Regulated Iq Motif-Containing Gtpase-Activating Protein Family in Liver Cancer

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