DNA methylation status of CRABP2 promoter down-regulates its expression

Zhang, Guimin; Song, Chengchuang; Li, Lijuan; He, Hongbin; Shi, Shu-Yue; Lei, Chuzhao; Li, Zheng; Peng, Shujun; Tian, Yi-Ran; Dang, Ruihua; Lan, Xianyong; Qi, Xinglei; Chen, Hong; Huang, Yongzhen

doi:10.1016/j.gene.2018.07.049

Cited by 5 publications

(5 citation statements)

References 28 publications

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“…As the methylation plays a vital role in THCA development and mRNA expression level, [ 22 , 23 ] we analyzed the methylation level of CHRDL1 through the data of TCGA. However, there was no difference in the CHRDL1 methylation level between THCA and normal tissues (Fig.…”

Section: Resultsmentioning

confidence: 99%

Bioinformatics identify the role of chordin-like 1 in thyroid cancer

Pang

Liu

et al. 2023

Medicine

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The abnormal expression of chordin-like 1 (CHRDL1) is identified in many cancers, while the effect of CHRDL1 in thyroid cancer (THCA) remains unclear. The University of California Santa Cruz, Gene Expression Profiling Interactive Analysis, University of Alabama at Birmingham Cancer, and Gene Expression Omnibus database (GSE33570, GSE33630, and GSE60542) were used for determining the mRNA and methylation expression of CHRDL1 in tumor and normal tissues. Human Protein Atlas was used for exploring the protein expression level of CHRDL1. The genes correlated to CHRDL1 were assessed by cBioPortal database. The prognostic value of CHRDL1 was evaluated through Kaplan–Meier method, cox regression, and nomogram analysis. Kyoto Encyclopedia of Genes and Genomes, Gene Ontology, and gene set enrichment analysis were used for predicting potential function of CHRDL1. The relationship between CHRDL1 and immune cell infiltration was determined by Pearson method. The downregulated mRNA and protein expressions of CHRDL1 were identified in THCA through the analysis of data from The Cancer Genome Atlas, Gene Expression Omnibus, and Human Protein Atlas database. The survival analysis showed that the CHRDL1 expression significantly affected disease-free interval (DFI) and progression-free interval, and CHRDL1 was an independent predictor of DFI. Besides, we found that C-C motif chemokine ligand 21 could significantly affect DFI time when it was co-expressed with CHRDL1. Additionally, the function of CHRDL1 was enriched in cell migration, apoptosis, and immune cell receptor. The downregulated expression of CHRDL1 was observed in THCA and caused poor prognosis. CHRDL1 may be involved in signal pathway related to cancer development and immune response, which suggested it could be a potential biomarker.

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Section: Resultsmentioning

confidence: 99%

Bioinformatics identify the role of chordin-like 1 in thyroid cancer

Pang

Liu

et al. 2023

Medicine

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“…CRABP2 has been known to be a central player in RA anticancer signal transduction (19, 20). However, this gene is often down-regulated or silenced by methylation and, therefore, results in RA tolerance in a variety of cancer cells (11–13, 21, 22). For instance, CRABP2 is silenced in the RA-resistant medulloblastoma UW228-2 cells due to CpG island methylation in the promoter region (12).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the status of CRABP2 expression is considered a critical element in determining RA sensitivity in cancer cells, including those of medulloblastoma and pancreatic cancers (11, 12). We have found that DNA methylation is responsible for the low or silenced CRABP2 expression in medulloblastoma cells (12, 13). DNMT inhibitor 5-aza-2′-deoxycytidinecytidine (5-aza) is thus able to restore CRABP2 expression and reverse RA resistance of UW228-2 medulloblastoma cells (12).…”

Section: Introductionmentioning

confidence: 93%

Resveratrol Reverses Retinoic Acid Resistance of Anaplastic Thyroid Cancer Cells via Demethylating CRABP2 Gene

Liu

et al. 2019

Front. Endocrinol.

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Background: Cellular retinoic acid binding protein 2 (CRABP2) mediates retinoic acid/RA anti-cancer pathways. Resveratrol effectively reverses RA tolerance and upregulates CRABP2 expression of anaplastic thyroid cancer cell line THJ-11T. As DNA methylation is responsible for CRABP2 silencing, the CRABP2 methylation status of THJ-11T cells and the demethylating effect of resveratrol on this gene are elucidated.Materials and methods: The statuses of CRABP2 expression and methylation and the levels of DNA methyltransferases (DNMTs) DNMT1, DNMT3A, and DNMT3B of THJ-11T cells were examined before and after resveratrol treatment via multiple experimental methods. The human medulloblastoma UW228-2 cell line was cited as the control of CRABP2 methylation and gemcitabine as the demethylator control.Results: RT-PCR, immunocytochemical staining and Western blotting showed that resveratrol significantly increased the CRABP2 expression and RA sensitivity of THJ-11T and UW228-2 cells. Bisulfite sequencing showed five CpG methylation sites at the CRABP2 promoter region of both cell lines, which were partially (3/5) demethylated by resveratrol and totally (5/5) by gemcitabine. DNMT1, DNMT3A, and DNMT3B were reduced in UW228-2 cells and DNMT1 and DNMT3A were reduced in THJ-11T cells after resveratrol treatment in a time-related fashion.Conclusion: Resveratrol is able to erase CRABP2 methylation and can thereby increase the RA sensitivity of THJ-11T and UW228-2 cells. This study demonstrates the additional value of the natural polyphenolic compound resveratrol as a demethylator in cancer treatments.

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“…Retinoic acid can regulate cell differentiation and proliferation by regulating the transcription of multiple target genes. Retinoic acid binding protein 2 (CRABP2) was a candidate gene affecting beef quality, yield and fat deposition [129]. The expression and methylation patterns of the CRABP2 promoter differential methylation region (DMR) in Jiaxian red bull, Jiaxian red bull × Angus and Jiaxian red bull × Simmental were studied [129].…”

Section: Studies On Dna Methylation Associated With Fat Deposition In...mentioning

confidence: 99%

“…Retinoic acid binding protein 2 (CRABP2) was a candidate gene affecting beef quality, yield and fat deposition [129]. The expression and methylation patterns of the CRABP2 promoter differential methylation region (DMR) in Jiaxian red bull, Jiaxian red bull × Angus and Jiaxian red bull × Simmental were studied [129]. It was found that there were different methylation and expression patterns of CRABP2 in different breeds and different tissues, and the methylation state of CRABP2 DMR could regulate the expression of other genes, which could be an important parameter for studying beef quality traits [129].…”

Section: Studies On Dna Methylation Associated With Fat Deposition In...mentioning

confidence: 99%

Effects of DNA Methylation on Gene Expression and Phenotypic Traits in Cattle: A Review

Zhang

Sheng

et al. 2023

IJMS

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Gene expression in cells is determined by the epigenetic state of chromatin. Therefore, the study of epigenetic changes is very important to understand the regulatory mechanism of genes at the molecular, cellular, tissue and organ levels. DNA methylation is one of the most studied epigenetic modifications, which plays an important role in maintaining genome stability and ensuring normal growth and development. Studies have shown that methylation levels in bovine primordial germ cells, the rearrangement of methylation during embryonic development and abnormal methylation during placental development are all closely related to their reproductive processes. In addition, the application of bovine male sterility and assisted reproductive technology is also related to DNA methylation. This review introduces the principle, development of detection methods and application conditions of DNA methylation, with emphasis on the relationship between DNA methylation dynamics and bovine spermatogenesis, embryonic development, disease resistance and muscle and fat development, in order to provide theoretical basis for the application of DNA methylation in cattle breeding in the future.

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DNA methylation status of CRABP2 promoter down-regulates its expression

Cited by 5 publications

References 28 publications

Bioinformatics identify the role of chordin-like 1 in thyroid cancer

Bioinformatics identify the role of chordin-like 1 in thyroid cancer

Resveratrol Reverses Retinoic Acid Resistance of Anaplastic Thyroid Cancer Cells via Demethylating CRABP2 Gene

Effects of DNA Methylation on Gene Expression and Phenotypic Traits in Cattle: A Review

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