DNA methyltransferase genes of Bacillus subtilis phages: comparison of their nucleotide sequences

Tran-Betcke, A; Behrens, B.; Noyer-Weidner, Mario; Trautner, Thomas A.

doi:10.1016/0378-1119(86)90153-8

Cited by 68 publications

(37 citation statements)

References 17 publications

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“…The third and largest cluster, which is likely to encompass the early phage operon(s), begins with the putative phage repressor gene yonR, whose product is 40 and 50 % identical to the PBSX repressor Xre (Krogh et al, 1996) and to the hypothetical transcriptional regulator YqaE of the skin element (Takemaru et al, 1995) , 1980;Tran-Betcke et al, 1986). Inspection of 12.5 kb EcoRI fragments of SPpc2 containing mtbP, inserted into pPS232, and cloned in E. coli TP610, a strain used for plasmid propagation, provides further confirmation of the role of MtbP.…”

Section: Gaur Et A! (1986) U32685mentioning

confidence: 99%

Nucleotide sequence of the Bacillus subtilis temperate bacteriophage SPβc2

et al. 1999

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The Bacillus subtilis 168 chromosomal region extending from 184" to 195", corresponding to prophage SPP, has been completely sequenced using DNA of the thermoinducible SPPc2 mutant. This 134416 bp segment comprises 187 putative ORFs which, according to their orientation, were grouped into three clusters. Compared to its host, SPPc2 is characterized by a lower G+C content, shorter mean ORF length, as well as a different usage of start codons. Nearly 75% of predicted ORFs do not share significant homologies to sequences in available databases. The only highly similar proteins to SPPc2-encoded ones are host paralogues. SPPc2 promoter regions contain 5 0 5 box consensus sequences and a repeated motif, designated SPP repeated element (SPBRE), that is absent from the host genome. Gene sspC, encoding the small acidsoluble protein C, that has been previously sequenced and mapped to the vicinity of the SPP region, was found to be part of the prophage.

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Section: Gaur Et A! (1986) U32685mentioning

confidence: 99%

Nucleotide sequence of the Bacillus subtilis temperate bacteriophage SPβc2

et al. 1999

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“…Besides, the antisense RNA technology has also been employed in C. acetobutylicum [32], [33]. However, all these genetic manipulations require methylation of the target vector in a strain bearing pAN1 [34] or pAN2 [30] containing a gene Ф 3tI encoding the Ф3TI methyltransferase from the phage Ф3TI of Bacillus subtilis [35]. The methylation step is necessary for transforming foreign DNA into C. acetobutylicum because of the presence of a type II restriction endonuclease Cac824I [22], which could recognize the GCNGC site.…”

Section: Introductionmentioning

confidence: 99%

Engineering Clostridium Strain to Accept Unmethylated DNA

et al. 2010

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It is difficult to genetically manipulate the medically and biotechnologically important genus Clostridium due to the existence of the restriction and modification (RM) systems. We identified and engineered the RM system of a model clostridial species, C. acetobutylicum, with the aim to allow the host to accept the unmethylated DNA efficiently. A gene CAC1502 putatively encoding the type II restriction endonuclease Cac824I was identified from the genome of C. acetobutylicum DSM1731, and disrupted using the ClosTron system based on group II intron insertion. The resulting strain SMB009 lost the type II restriction endonuclease activity, and can be transformed with unmethylated DNA as efficiently as with methylated DNA. The strategy reported here makes it easy to genetically modify the clostridial species using unmethylated DNA, which will help to advance the understanding of the clostridial physiology from the molecular level.

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“…Table 1 shows that compared to uncleaved DNA, partial or total in uitro restriction of the donor DNA reduced or abolished its potential to generate BsuR-resistant phages, although none of the enzymes used cleaves within the Mtase genes (Noyer-Weidner et al, 1983, and authors' unpublished observations). This finding can be rationalized on the basis of the size of the Mtase-gene-carrying fragments (Table 1) and, in the case of 43T and SPR, where mapping and/or sequencing data are available (Noyer-Weidner et al, 1985;Tran-Betcke et al, 1986), the location of the Mtase genes within these fragments. Phages obtained from singleplaque isolates proved to be recombinants since their resistance to BsuR restriction was stably acquired.…”

Section: Resultsmentioning

confidence: 99%

Recombinant Derivatives of Bacillus Subtilis Phage Z Containing the DNA Methyltransferase Genes of Related Methylation-proficient Phages

Terschüren

Noyer-Weidner²,

Trautner³

1987

Microbiology

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The DNA methyltransferase (Mtase) genes of temperate Bacillus subtilis phages SPR, 43T, SPP and pl 1 can be transferred by transfection and recombination to the genome of the related nonmodifying phage Z. Integration of the Mtase genes occurs in phage Z DNA at a unique location which is homologous with the flanking regions of the Mtase genes of the related phages. In lysogenic cells carrying recombinant phages, expression of the Mtase genes is repressed, irrespective of whether the Mtase genes were derived from phage donors which were homo-or heteroimmune to phage Z.

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DNA methyltransferase genes of Bacillus subtilis phages: comparison of their nucleotide sequences

Cited by 68 publications

References 17 publications

Nucleotide sequence of the Bacillus subtilis temperate bacteriophage SPβc2

Nucleotide sequence of the Bacillus subtilis temperate bacteriophage SPβc2

Engineering Clostridium Strain to Accept Unmethylated DNA

Recombinant Derivatives of Bacillus Subtilis Phage Z Containing the DNA Methyltransferase Genes of Related Methylation-proficient Phages

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