DNA microarray analysis of genomic responses of yeast Saccharomyces cerevisiae to nickel chloride

Abstract

-In order to investigate nickel toxicity against the yeast Saccharomyces cerevisiae, genomic responses to nickel chloride were examined using yeast DNA microarrays. Microarray analysis revealed that exposure to 25 mM nickel chloride for 2 hr induced changes in gene expression in S. cerevisiae. Nickel chloride increased expression levels in 601 genes and decreased expression levels in 696 genes in S. cerevisiae.

Evolutionary engineering and transcriptomic analysis of nickel-resistantSaccharomyces cerevisiae

“…Exposure of S. cerevisiae cells grown in YPD medium to a pulse stress of 25 mM NiCl 2 followed by DNA microarray analysis revealed that genes related to sulfur amino acid metabolism, iron metabolism, and heavy metal homeostasis were significantly upregulated. The upregulated gene groups that are common to both M9 and the reference strain in Takumi's work are those related to iron metabolism, metal homeostasis, cellular response to oxidative stress, and xenobiotics resistance (GTT1 and GTT2) ( Table 2, and Takumi et al, 2010). Transcriptomic data were similar to those obtained previously where cobalt stress was shown to induce iron accumulation in yeast cells (Stadler & Schweyen, 2002).…”

“…The results of the physiological and transcriptomic analyses of a nickel hyper-resistant S. cerevisiae mutant were compared with the results of previous studies with metal-sensitive deletion mutants and/or nickel-exposed reference strains of S. cerevisiae, as well as with a cobalt hyper-resistant S. cerevisiae mutant obtained by our group (C ßakar et al, 2009;Alkim et al, 2013). In the present study on nickel hyper-resistant mutant M9, however, only 4 (GTT2, MHT1, STR2, and STR3) of 26 upregulated genes related to sulfur amino acid metabolism in nickel-exposed S288C (Takumi et al, 2010) were found to be also upregulated in M9, and the level of upregulation of those genes in M9 was significantly lower than in S288C. Exposure of S. cerevisiae cells grown in YPD medium to a pulse stress of 25 mM NiCl 2 followed by DNA microarray analysis revealed that genes related to sulfur amino acid metabolism, iron metabolism, and heavy metal homeostasis were significantly upregulated.…”

“…Takumi et al (2010) investigated transcriptomic responses of a reference laboratory strain of S. cerevisiae (S288C) to NiCl 2 using yeast DNA microarrays. Besides, stress response genes, genes related to protein refolding; trehalose, glucose, and amino acid metabolism; and glycogen biosynthesis, were upregulated in M9, but they were not found to be upregulated in the reference strain of Takumi et al (2010) upon nickel exposure. Additionally, genes related to oxidative stress response, that is, glutathione peroxidase, thioredoxin and glutaredoxin, and DNA repair were induced.…”

Transition metal homeostasis: from yeast to human disease

“…Among the many experiments where S. cerevisiae has proved to be a useful model organism are then many high throughput studies that have aided in the identification of genes and gene products with novel implications in transition metal metabolism (for examples see Pagani et al 2007;Jo et al 2008;Arita et al 2009;Shakoury-Elizeh et al 2010;Takumi et al 2010;Bleackley et al 2011). The contribution of yeast based studies and the identification of homologous systems in humans will continue to facilitate advances in the diagnosis and treatment of diseases involving transition metals while also contributing to the general understanding of how cells maintain the balance of metals as essential nutrients versus toxic entities.…”

Yeast toxicogenomics: genome-wide responses to chemical stresses with impact in environmental health, pharmacology, and biotechnology

“…However the genome-wide yeast response to toxic concentrations of metal ions, such as nickel, cadmium, copper, chromium, arsenic, cobalt, manganese, and zinc has been studied by exploring chemogenomics and transcriptomics approaches. These studies led to the identification of several functional groups that are important in the yeast response to all or to part of the metal ions tested, mostly involved in sulfur amino acid and iron metabolism, oxidative stress response, vacuolar function, protein modification, transport and degradation, enzyme inactivation, cation and transition metal transport, mRNA decay, and DNA metabolism (Momose and Iwahashi, 2001; Jin et al, 2008; Ruotolo et al, 2008; Serero et al, 2008; Yasokawa et al, 2008; Takumi et al, 2010; Bleackley et al, 2011). The toxicological outcome of the exposure to agrochemicals, including herbicides and agricultural fungicides, is difficult to predict, since many times it takes years to develop.…”