DNA microarray analysis of the heat- and cold-shock stimulons in Yersinia pestis

Han, Yanping; Zhou, Dongsheng; Pang, Xin; Zhang, Ling; Song, Yajun; Tong, Zongzhong; Bao, Jingyue; Dai, Erfu; Wang, Jin; Guo, Zhaobiao; Zhai, Jing; Du, Zongmin; Wang, Xiaoyi; Wang, Jian; Huang, Peitang; Yang, Ruifu

doi:10.1016/j.micinf.2004.11.005

Cited by 61 publications

(61 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Consistent with this hypothesis, Y. pestis grown at 5°C prior to infection has the lowest virulence for animals among all cultures tested that were grown within the temperature interval of 5°C to 41°C (38). It has also been noted that the main bacterial virulence factors responsible for Y. pestis pathogenicity, such as Caf1, PsaA, Yops, Yscs, Pla, etc., as well as the insecticidal toxin complex TccC, were downregulated under cold growth conditions (24) Overall, our studies revealed a partial correlation between temperature-dependent variations in the LPS structures in representatives of various Y. pestis strains and their susceptibilities to killing by PMB and serum. The biological significance of all of the temperature-dependent LPS alterations described for Y. pestis subspecies is not completely clear, and one should take into account that growing bacteria under laboratory conditions may not accurately model the in vivo conditions.…”

Section: Vol 73 2005 Y Pestis Susceptibilities To Serum and Polymymentioning

confidence: 57%

“…pestis has only rarely been studied at temperatures below 20°C (24,35,38). However, 5°C is approximately the core body temperature of Y. pestis rodent hosts undergoing seasonal hibernation (marmots and ground squirrels), when their body temperature is maintained 1°C to 2°C above the ambient temperature (30,31,40).…”

Section: Vol 73 2005 Y Pestis Susceptibilities To Serum and Polymymentioning

confidence: 99%

See 1 more Smart Citation

Intraspecies and Temperature-Dependent Variations in Susceptibility of Yersinia pestis to the Bactericidal Action of Serum and to Polymyxin B

et al. 2005

View full text Add to dashboard Cite

Lipopolysaccharide (LPS) structure impacts the bactericidal action of cationic peptides, such as polymyxin B (PMB), and sensitivity to killing by normal human serum (NHS). Cultivation of different subspecies strains of Yersinia pestis isolated from unrelated geographic origins at various temperatures (mammals, 37°C; fleas, 25°C; or winter hibernation, 6°C) affects LPS composition and structure. We tested the susceptibilities of various strains of Y. pestis grown at these different temperatures to PMB and serum bactericidal killing. Both properties varied significantly in response to temperature changes. In Y. pestis subsp. pestis (the main subspecies causing human plague), high levels of resistance to PMB and NHS were detected at 25°C. However, at the same temperature, Y. pestis subsp. caucasica was highly sensitive to PMB. At both of the extreme temperatures, all strains were highly susceptible to PMB. At 25°C and 37°C, Y. pestis subsp. caucasica strain 1146 was highly susceptible to the bactericidal activity of 80% NHS. All Y. pestis strains studied were able to grow in heatinactivated human serum or in 80% normal mouse serum. At 6°C, all strains were highly sensitive to NHS. Variations in the PMB resistance of different bacterial cultures related to both the content of cationic components (4-amino-4-deoxyarabinose in lipid A and glycine in the core) and a proper combination of terminal monosaccharides in the LPS. The NHS resistance correlated with an elevated content of N-acetylglucosamine in the LPS. Structural variation in the LPS of Y. pestis correlates with the organism's ability to resist innate immunity in both fleas and mammals.

show abstract

Section: Vol 73 2005 Y Pestis Susceptibilities To Serum and Polymymentioning

confidence: 57%

Section: Vol 73 2005 Y Pestis Susceptibilities To Serum and Polymymentioning

confidence: 99%

Intraspecies and Temperature-Dependent Variations in Susceptibility of Yersinia pestis to the Bactericidal Action of Serum and to Polymyxin B

et al. 2005

View full text Add to dashboard Cite

show abstract

“…A total of 153 virulence-associated proteins were selected according to the annotated genome of Y. pestis CO92 (58) and the current literature regarding Yersinia pathogenesis (see Table S1 in the supplemental material). Bait proteins were selected according to the following criteria (with the numbers of bait proteins in each category indicated in parentheses): (i) all published virulence factors, such as the type III Yop effectors, pH 6 antigen, Pla protease, Ail, and F1 antigen (n ϭ 12); (ii) genes that are intact in fully virulent CO92 but are disrupted in 91001 (n ϭ 9), a biovar microtus strain that is avirulent to humans but highly virulent to mice (58,70); (iii) genes that are actively regulated in response to various adverse in vitro stimuli or inside infected cells (n ϭ 13) (31,32,47,82); (iv) genes encoding proteins that trigger antibody responses in infected rodents or humans (n ϭ 17) (44, 45); (v) some genes that encode putative membrane protein, exported protein, or surface structures according to the annotated genome of CO92; (vi) other genes, including some genes that are annotated to be related to pathogenicity, detoxification, and mobility; and (vii) genes located in the three virulence-associated plasmids pCD1, pMT1, and pPCP1 (n ϭ 69). The full-length sequences of all of the bait proteins except YPCD1.72c (YpkA) were cloned into pDEST22 using Gateway recombination technology (77).…”

Section: Resultsmentioning

confidence: 99%

Insight into Bacterial Virulence Mechanisms against Host Immune Response via the Yersinia pestis-Human Protein-Protein Interaction Network

et al. 2011

Self Cite

View full text Add to dashboard Cite

“…The most striking feature is the paradoxical upregulation of the GroEL2 transcript. This transcript encodes a member of the HSP family and is expected to be down-regulated at low temperatures (5,25). While unconventional, an increase in transcription of HSP60 genes at low temperatures was previously described for the hyperthermoacidophilic archaeon Sulfolobus shibatae, which can adapt to and survive in extreme natural environments (30).…”

Section: Figmentioning

confidence: 87%

Global Transcriptome Analysis ofTropheryma whippleiin Response to Temperature Stresses

et al. 2006

View full text Add to dashboard Cite

Tropheryma whipplei, the agent responsible for Whipple disease, is a poorly known pathogen suspected to have an environmental origin. The availability of the sequence of the 0.92-Mb genome of this organism made a global gene expression analysis in response to thermal stresses feasible, which resulted in unique transcription profiles. A few genes were differentially transcribed after 15 min of exposure at 43°C. The effects observed included up-regulation of the dnaK regulon, which is composed of six genes and is likely to be under control of two HspR-associated inverted repeats (HAIR motifs) found in the 5 region. Putative virulence factors, like the RibC and IspDF proteins, were also overexpressed. While it was not affected much by heat shock, the T. whipplei transcriptome was strongly modified following cold shock at 4°C. For the 149 genes that were differentially transcribed, eight regulons were identified, and one of them was composed of five genes exhibiting similarity with genes encoding ABC transporters. Up-regulation of these genes suggested that there was an increase in nutrient uptake when the bacterium was exposed to cold stress. As observed for other bacterial species, the major classes of differentially transcribed genes encode membrane proteins and enzymes involved in fatty acid biosynthesis, indicating that membrane modifications are critical. Paradoxically, the heat shock proteins GroEL2 and ClpP1 were up-regulated. Altogether, the data show that despite the lack of classical regulation pathways, T. whipplei exhibits an adaptive response to thermal stresses which is consistent with its specific environmental origin and could allow survival under cold conditions.

show abstract

DNA microarray analysis of the heat- and cold-shock stimulons in Yersinia pestis

Cited by 61 publications

References 48 publications

Intraspecies and Temperature-Dependent Variations in Susceptibility of Yersinia pestis to the Bactericidal Action of Serum and to Polymyxin B

Intraspecies and Temperature-Dependent Variations in Susceptibility of Yersinia pestis to the Bactericidal Action of Serum and to Polymyxin B

Insight into Bacterial Virulence Mechanisms against Host Immune Response via the Yersinia pestis-Human Protein-Protein Interaction Network

Global Transcriptome Analysis ofTropheryma whippleiin Response to Temperature Stresses

Contact Info

Product

Resources

About