DNA modifications as a means of detecting the irradiation of wheat

Jabir, A.W.; Deeble, David J.; Wheatley, P.A.; Smith, Cecile; Parsons, Barry J.; Beaumont, Paul C.; Swallow, A. J.

doi:10.1016/1359-0197(89)90331-7

Cited by 5 publications

(12 citation statements)

References 12 publications

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“…Jabir et al (1989) used the fluorometric assay to examine the thymine glycol content of DNA isolated from irradiated 662-JOURNAL OF FOOD SCIENCE-Volume 57, No. 3, 1992 wheat and reported a "reasonable increase" in yield of thymine glycol with increasing dose.…”

Section: Resultsmentioning

confidence: 99%

Ineffectiveness of a Fluorometric Method for Identifying Irradiated Food Based on Thymine Glycol

Ewing

Stepanik

1992

Journal of Food Science

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At dosages used for food irradiation, some of the thymine present in the DNA of irradiated food may be converted to thymine glycol. A fluorometric assay for thymine glycol was investigated as a possible method of detecting irradiated foods based on this effect. Experiments were performed on homogenates of irradiated chicken breast meat and on DNA isolated from irradiated chicken breast meat. In both cases the assay was subject to interference from one of the reagents, oaminobenzaldehyde, and lacked the necessary sensitivity to detect the thymine glycol produced by radiolysis of the DNA at relevant dosages.

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Section: Resultsmentioning

confidence: 99%

Ineffectiveness of a Fluorometric Method for Identifying Irradiated Food Based on Thymine Glycol

Ewing

Stepanik

1992

Journal of Food Science

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“…ELISA methods are capable of determining concentrations of particular compounds in the picomolar to femtomolar range and thus there is the possibility of omitting the DNA extraction step and carrying out the assay on heated (to denature the DNA) homogenates of food. Preliminary experiments with wheat (Jabir et al 1989) did not show the dose-dependent amounts of thymidine glycol found using fluorescence assays and further development in the selection and raising of appropriate antibodies is necessary.…”

Section: Dna Methodsmentioning

confidence: 93%

Detection of irradiated food: A review

Glidewell¹,

Deighton²,

Goodman³

et al. 1993

J Sci Food Agric

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Abstract:The sale of irradiated food in the UK has been legal since the start of 1991 with the proviso that it must be labelled as such. At present there are no methods sufficiently accurate and reliable for the routine detection of irradiated food of all varieties. The current state of development of different physical, chemical and biological methods available is reviewed. These comprise electron paramagnetic resonance, luminescence, viscosity changes, detection of radiationinduced chemical species such as volatile hydrocarbons and o-tyrosine by chromatographic-mass spectrometric techniques, changes to DNA and changes in micro flora.

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“…The second approach is the chemical way, which is performed by GC-MS (Gas Chromatography-Mass Spectrometry) (Stevenson et al, 1993). The third means of inspecting irradiation of food products is the biological way, which utilizes the Comet assay (Jabir et al, 1989;Kumu et al, 1994). Since TL and PSL are typically used to assess the food which contains minerals, TL can be used after minerals are extracted.…”

Section: Introductionmentioning

confidence: 99%