DNA multigene sequencing of topotypic specimens of the fascioliasis vector Lymnaea diaphana and phylogenetic analysis of the genus Pectinidens (Gastropoda)

Bargues, M. D.; Sierra, Roberto; Artigas, Patricio; Mas‐Coma, Santiago

doi:10.1590/s0074-02762012000100016

Cited by 11 publications

(5 citation statements)

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“…The target 16S gene region was amplified by PCR using a set of universal primers [ 53 ]. Amplification procedures and thermal cycler conditions were carried out as previously described for lymnaeids [ 38 , 42 , 44 ]. A mitochondrial DNA cox 1 gene fragment was amplified using universal primers [ 54 ].…”

Section: Methodsmentioning

confidence: 99%

Molecular characterisation of Galba truncatula, Lymnaea neotropica and L. schirazensis from Cajamarca, Peru and their potential role in transmission of human and animal fascioliasis

et al. 2012

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BackgroundHuman and animal fascioliasis is emerging in many world regions, among which Andean countries constitute the largest regional hot spot and Peru the country presenting more human endemic areas. A survey was undertaken on the lymnaeid snails inhabiting the hyperendemic area of Cajamarca, where human prevalences are the highest known among the areas presenting a "valley transmission pattern", to establish which species are present, genetically characterise their populations by comparison with other human endemic areas, and discuss which ones have transmission capacity and their potential implications with human and animal infection.MethodsTherefore, ribosomal DNA ITS-2 and ITS-1, and mitochondrial DNA 16S and cox1 were sequenced by the dideoxy chain-termination method.ResultsResults indicate the presence of three, morphologically similar, small lymnaeid species belonging to the Galba/Fossaria group: Galba truncatula, Lymnaea neotropica and L. schirazensis. Only one combined haplotype for each species was found. The ITS-1, 16S and cox1 haplotypes of G. truncatula are new. No new haplotypes were found in the other two species. This scenario changes previous knowledge, in which only L. viator (= L. viatrix) was mentioned. Galba truncatula appears to be the most abundant, with high population densities and evident anthropophyly including usual presence in human neighbourhood. Infection by Fasciola hepatica larval stages were molecularly confirmed in two populations of this species. The nearness between G. truncatula populations presenting liver fluke infection and both human settings and schools for children, together with the absence of populations of other lymnaeid species in the locality, suggest a direct relationship with human infection.ConclusionsThe geographical overlap of three lymnaeid species poses problems for epidemiological studies and control action. First, a problem in classifying lymnaeid specimens in both field and laboratory activities, given their transmission capacity differences: G. truncatula mainly involved in transmission to humans, L neotropica typically responsible for livestock infection, and L. schirazensis unable for transmission. Although several phenotypic characteristics may be helpful for a preliminary specimen classification, a definitive classification can only be obtained by marker sequencing. Aditionally, L. schirazensis increases the confusion, owing to its ability to mix with other Galba/Fossaria species and distort fascioliasis data such as transmission capacity and infection susceptibility. Second, a problem for epidemiological analysis, surveillance and control by methods as mathematical modelling and Remote Sensing - Geographical Information Systems. In Cajamarca, low resolution mapping may be insufficient, as already verified in Andean areas where different lymnaeid species overlap.

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Section: Methodsmentioning

confidence: 99%

Molecular characterisation of Galba truncatula, Lymnaea neotropica and L. schirazensis from Cajamarca, Peru and their potential role in transmission of human and animal fascioliasis

et al. 2012

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“…Sources: Bargues and Mas-Coma 1997 (Z73980-5); Bargues et al 1997 (Z83831); Bargues et al 2007 (AM412222); Bargues et al 2009 (FN182190); Bargues et al 2011a (FR772291); Bargues et al 2011b (FN598151-2); Bargues et al 2012 (JF909497); Correa et al 2011 (JN614326-68); Dayrat et al 2011 (HQ659965-6); Duffy et al 2009 (AF239912, AY057088-9, EU241865-6, EU728668); Jorgensen et al 2004 (AY577484); Klamath River at Collier Rest Area, California (HM230307*); Klussmann-Kolb et al 2008 (EF489345) (Note: their analyses excluded variable regions, so perhaps the region was excised from the published sequence rather than originally absent); Marquez, unpublished (Y09015-9); Owyhee River, Whistling Bird Rapids, Oregon (HM230306*); Stothard et al 2000 (AF192272-4); Vinarski et al 2011 (FR797815-29) Vonnemann et al 2005 (AY427525); Walker et al 2008 (EU152269, EU152270). Only the E10 region is considered above, so there may be differences in other parts of the sequence for ones that are grouped in the table.…”

Section: Resultsmentioning

confidence: 99%

Phylogenetic analysis of the Lancinae (Gastropoda, Lymnaeidae) with a description of the U.S. federally endangered Banbury Springs lanx

Campbell¹,

Clark²,

Lydeard³

2017

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We examined the patelliform snails of the subfamily Lancinae, endemic to northwestern North America, to test whether morphological variation correlated with genetic and anatomical differences. Molecular analyses using cox1, 16S, calmodulin intron, and 28S rDNA partial sequences and anatomical data supported recognition of four species in three genera. The relationships of lancines within Lymnaeidae are not yet well-resolved. The federally endangered Banbury Springs lanx is described as a new genus and species, Idaholanx fresti, confirming its distinctiveness and narrow endemicity.

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“…Other South American Lymnaeidae are Lymnaea diaphana and Lymnaea rupestris. The former species is described to serve as a host of F. hepatica in the southern part of the Chilean and Argentinean Patagonia [47]. The role of the second species L. rupestris in the transmission of F. hepatica is not known [104].…”

Section: South American Lymnaeidaementioning

confidence: 99%

Lymnaeid snails hosts of Fasciola hepatica and Fasciola gigantica (Trematoda: Digenea): a worldwide review.

Vázquez¹,

Alda²,

Lounnas³

et al. 2019

CABI Reviews

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Fasciolosis is a snail-borne disease, causing serious public and veterinary health problems worldwide. This disease is produced by infection with Fasciola hepatica or Fasciola gigantica through the consumption of vegetables or water contaminated with the parasite's metacercarial cysts. Both species of liver flukes are transmitted worldwide by small freshwater snails of the family Lymnaeidae. A global account on the species that are actually or may act as potential hosts of Fasciola spp., compiling particular research on their geographical distribution and susceptibility, is needed as a helpful tool in the understanding of fasciolosis transmission, and therefore in the control and prevention programmes of the disease. We have gathered here a comprehensive review of those lymnaeid species that are known to transmit the parasites in the field or that have been experimentally tested. We aim to bring forward the main intermediate hosts by regions in order to facilitate the understanding of worldwide transmission.

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DNA multigene sequencing of topotypic specimens of the fascioliasis vector Lymnaea diaphana and phylogenetic analysis of the genus Pectinidens (Gastropoda)

Cited by 11 publications

References 60 publications

Molecular characterisation of Galba truncatula, Lymnaea neotropica and L. schirazensis from Cajamarca, Peru and their potential role in transmission of human and animal fascioliasis

Molecular characterisation of Galba truncatula, Lymnaea neotropica and L. schirazensis from Cajamarca, Peru and their potential role in transmission of human and animal fascioliasis

Phylogenetic analysis of the Lancinae (Gastropoda, Lymnaeidae) with a description of the U.S. federally endangered Banbury Springs lanx

Lymnaeid snails hosts of Fasciola hepatica and Fasciola gigantica (Trematoda: Digenea): a worldwide review.

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