DNA Nanobiosensors: An Outlook on Signal Readout Strategies

Chandrasekaran, Arun Richard

doi:10.1155/2017/2820619

Cited by 25 publications

(16 citation statements)

References 92 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This unprecedented addressability of the DNA origami approach allows arranging different biosensing components, introducing new biorecognition elements and multiplexing strategies, as well as the implementation of signal transduction and amplification mechanisms. Using DNA origami, a number of biosensors have been developed capable of single-molecule detection of DNA and RNA (Ke et al, 2008;Zhang et al, 2010a;Kuzuya et al, 2011;Ochmann et al, 2017;Selnihhin et al, 2018;Funck et al, 2018;Trofymchuk et al, 2021), single nucleotide polymorphisms (Zhang et al, 2010b;Subramanian et al, 2011), specific metal ions (Ke et al, 2008;Marras et al, 2018), as well as various protein biomarkers (Rinker et al, 2008;Koirala et al, 2014;Godonoga et al, 2016;Raveendran et al, 2020) among many others (Wang et al, 2017a(Wang et al, , 2017b(Wang et al, , 2020Chandrasekaran, 2017;Ke et al, 2018;Loretan et al, 2020;Dass et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Single antibody detection in a DNA origami nanoantenna

et al. 2021

View full text Add to dashboard Cite

DNA nanotechnology offers new biosensing approaches by templating different sensor and transducer components. Here, we combine DNA origami nanoantennas with label-free antibody detection by incorporating a nanoswitch in the plasmonic hotspot of the nanoantenna. The nanoswitch contains two antigens that are displaced by antibody binding, thereby eliciting a fluorescent signal. Singleantibody detection is demonstrated with a DNA origami integrated anti-digoxigenin antibody nanoswitch. In combination with the nanoantenna, the signal generated by the antibody is additionally amplified. This allows the detection of single antibodies on a portable smartphone microscope. Overall, fluorescence-enhanced antibody detection in DNA origami nanoantennas shows that fluorescence-enhanced biosensing can be expanded beyond the scope of the nucleic acids realm.

show abstract

Section: Introductionmentioning

confidence: 99%

Single antibody detection in a DNA origami nanoantenna

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Recently, DNA nanostructures have garnered tremendous interest in biosensing due to their high surface-to-volume ratio, which provides greater space for responsive elements and therefore greater changes in signal generation. 251 This biosensing ability has been explored for the early detection of pathogens in human samples 262 with enhanced target specificity and avidity. 263 In this section, aptasensors utilizing a DNA nanostructure scaffold are discussed, categorizing the techniques based on the same output methods discussed previously.…”

Section: Role Of Dna Nanotechnology In Viral Diagnostics and Therapymentioning

confidence: 99%

Aptamers for Viral Detection and Inhibition

et al. 2022

Self Cite

View full text Add to dashboard Cite

Recent times have experienced more than ever the impact of viral infections in humans. Viral infections are known to cause diseases not only in humans but also in plants and animals. Here, we have compiled the literature review of aptamers selected and used for detection and inhibition of viral infections in all three categories: humans, animals, and plants. This review gives an in-depth introduction to aptamers, different types of aptamer selection (SELEX) methodologies, the benefits of using aptamers over commonly used antibody-based strategies, and the structural and functional mechanism of aptasensors for viral detection and therapy. The review is organized based on the different characterization and read-out tools used to detect virus–aptasensor interactions with a detailed index of existing virus-targeting aptamers. Along with addressing recent developments, we also discuss a way forward with aptamers for DNA nanotechnology-based detection and treatment of viral diseases. Overall, this review will serve as a comprehensive resource for aptamer-based strategies in viral diagnostics and treatment.

show abstract

“…These signal readers are integrated electronically with smart gadgets or plate readers to measure fluorescence. The improvement in signal detection methods improves the sensitivity of the procedure [ 110 ]. The conventional signal readout method is highly delicate to interpret the final results.…”

Section: Detection Techniques Of Sars-cov-2mentioning

confidence: 99%

Advancements in Testing Strategies for COVID-19

et al. 2022

View full text Add to dashboard Cite

The SARS-CoV-2 coronavirus, also known as the disease-causing agent for COVID-19, is a virulent pathogen that may infect people and certain animals. The global spread of COVID-19 and its emerging variation necessitates the development of rapid, reliable, simple, and low-cost diagnostic tools. Many methodologies and devices have been developed for the highly sensitive, selective, cost-effective, and rapid diagnosis of COVID-19. This review organizes the diagnosis platforms into four groups: imaging, molecular-based detection, serological testing, and biosensors. Each platform’s principle, advancement, utilization, and challenges for monitoring SARS-CoV-2 are discussed in detail. In addition, an overview of the impact of variants on detection, commercially available kits, and readout signal analysis has been presented. This review will expand our understanding of developing advanced diagnostic approaches to evolve into susceptible, precise, and reproducible technologies to combat any future outbreak.

show abstract

DNA Nanobiosensors: An Outlook on Signal Readout Strategies

Cited by 25 publications

References 92 publications

Single antibody detection in a DNA origami nanoantenna

Single antibody detection in a DNA origami nanoantenna

Aptamers for Viral Detection and Inhibition

Advancements in Testing Strategies for COVID-19

Contact Info

Product

Resources

About