DNA‐Nanostructure–Gold‐Nanorod Hybrids for Enhanced In Vivo Optoacoustic Imaging and Photothermal Therapy

Abstract

A functional cancer theranostic nanoplatform is developed, specifically tailored toward the optoacoustic modality by combining gold nanorods with DNA nanostructures (D-AuNR). DNA origami is used as an efficient delivery vehicle owing to its prominent tumor-targeting property. The D-AuNR hybrids display an enhanced tumor diagnostic sensitivity by improved optoacoustic imaging and excellent photothermal therapeutic properties in vivo.

Advances in DNA Origami–Cell Interfaces

“…ChemBioChem 2020, 21,[33][34][35][36][37][38][39][40][41][42][43][44] www.chembiochem.org increased by around 15 %( from 68.3, 71.8 and 70.5 %t o8 5.8, 86.2 and 86.3 %f or cross, rectangle and triangle shapes,r espectively) if the reactionw as performed at an elevated temperatureo f3 78C, relative to the results obtained at commonly used room temperature.…”

“…Building on this, Du and co‐workers used such a system to combine photothermal therapy with optoacoustic imaging to also enable diagnostic imaging functionality . In their excellent review on the topic, Li and Chen explain that optoacoustic imaging (also called photoacoustic imaging) is an imaging technique in which a laser light is directed onto the tissue to be imaged and endo‐ or exogenous chromophores adsorb this light and then release it as heat, causing a rapid thermoelastic expansion, which generates a wide‐band ultrasound wave that can then be detected and processed to give an image .…”

“…Gold nanoparticles have a strong and adjustable optical absorption, resulting from the surface plasmon effect, in which free charges on the surface of the nanoparticle oscillate with the electromagnetic field, and therefore, have been widely used as contrast agents for photoacoustic imaging. Du and co‐workers reported a large difference in contrast ratio between D‐GNR (DNA origami nanostructures loaded with GNRs) and naked GNRs; simultaneously, GNRs responded to near‐infrared irradiation, which enabled photothermal therapy and effectively inhibited tumour regrowth and prolonged the survival of diseased mice …”

Self‐Assembled DNA Nanostructures for Biomedical Applications

“…Thereafter, Ding's group demonstrated that the DO‐AuNR complex showed a significant photoacoustic imaging effect (Figure c). The accumulation of DO‐AuNR in tumor was confirmed by dark‐field images and inductively coupled plasma‐mass spectrometry (ICP‐MS), which indicated the promising application of DO‐AuNR in tumor diagnosis …”

Multifunctional DNA Origami Nanoplatforms for Drug Delivery

“…After observing the efficient photothermal therapy effect based on the DNA origami-GNR complex, Tian and co-workers subsequently developed GNR-loaded triangular DNA origami to function as an optoacoustic imaging agent. [35] GNR-loaded DNA origami can act as an efficient contrasta gent to achieve enhanced imaging quality with ad ecreased dose. Photothermal treatment based on this DNA origami-GNR complexd emonstrated efficient inhibition of tumor growth in vivo (Figure 3b).…”