DNA Nanoswitch as a Biosensor

Buck, Amy H.; Campbell, Colin J.; Dickinson, Paul; Mountford, C. P.; Stoquert, Helene C; Terry, Jonathan G.; Evans, Stuart A. G.; Keane, Lorraine; Su, Tsueu-Ju; Mount, Andrew R.; Walton, A.J.; Beattie, John S.; Crain, Jason; Ghazal, Peter

doi:10.1021/ac070251r

Cited by 24 publications

(31 citation statements)

References 30 publications

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“…The recent work of Crain's group has presented a new method to discriminate single-base mismatches based on DNA nanoswitches. 93,94 In their design, the probe and target DNA will form a switchable four-way Holliday junction. A remarkable characteristic of this complex is its ability to switch in response to stimuli such as divalent ions.…”

Section: As Sensorsmentioning

confidence: 99%

DNA nanomachines and their functional evolution

2009

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Since the establishment of the Watson-Crick model more than five decades ago, the understandings of DNA structures are well sufficient to enable applications of DNA in designing and assembling two-dimensional (2D) and three-dimensional (3D) structures at the nanoscale. Furthermore, the conformational switchability of DNA also enables the fabrication of nanoscale molecular machines, which can perform movements upon stimuli. In this article, we will summarize the present efforts on constructions of DNA nanomachines based on different driven mechanisms, and further discuss their evolutional processes, in order to find applications and future development directions.

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Section: As Sensorsmentioning

confidence: 99%

DNA nanomachines and their functional evolution

2009

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“…The interaction between doctors and medical representatives through gift-giving is the most common reason for conflicts of interest for doctors as it negatively influences their prescribing behaviors all over the world (Mikhael & Alhilali, 2014). Doctor's connections with the industry build complex relations, influence physician's decisions and prescribing patterns, and damage patient's trust in the profession (Buck et al, 2007). Green et al, (2012) mentioned that the receipt of a gift could create doubts in respect of physician credibility and it is perceived as problematic by the patients.…”

Section: Unethical Marketing Practicesmentioning

confidence: 99%

Challenges of Pakistani Pharmaceutical Industry: Pakistan Case

Ahmed¹,

Chandani²

2020

IJELCS

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The population of Pakistan is fast growing and the need for supply of medicine will continue to increase. The local pharmaceutical market is growing at a faster pace as compared with the international market. Until 1990, the reliance on the supply of medicine to cater to the national demand was on multinational companies holding major market share. Thereafter, the national pharmaceutical companies started investing to improve the quality of their product lines, employing qualif ied professionals, and ensuring compliance with the global standard of quality and good manufacturing practices. Consequently, national com- panies' market share drastically increased and the national demand for medicine is locally produced. The study results based on interviews of key stakeholders and available literature revealed that the local pharmaceutical sector is facing critical challenges of counterfeit medicine, pricing controversies, affordability of the medicine, lack of Research and Development (R&D) initiatives, and unethical marketing (bribing/cash incentives to the doctors). The Drug Regulatory Authority of Pakistan (DRAP) seems ineffective to overcome these challenges, eliminate counterfeit medicines, and take measures to curb the unethical marketing practices that are risking patient life, health, and treatment cost. The study further explored that unethical marketing practices, and prescribing expensive brands by doctors, creates a serious conflict of interests and fast a decline in patient trust and affordability of medicine cost. Thus strong regulatory controls,transparency, moral and ethical values are needed to enforce drug acts and make the stakeholder's groups accountable. There is a need to punish both the companies bribing the doctors on the pretext of product promotion and doctors accepting such benef its to protect the patient's interest and limit treatment cost. Strict regulations and incentive plans for the pharmaceutical sector are needed to promote Research and Development (R&D).

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“…133 To circumvent the issues of allele-specic hybridization, new SNP-typing methods are currently being developed that do not rely on the differences in thermodynamic stability for discrimination. For instance, SNPs have been detected by charge transport through the p-stack of DNA duplexes, 143,144 base-discriminating uorescent nucleosides, 145,146 kinetic schemes, [147][148][149][150][151][152] and mismatch-binding ligands. [153][154][155] While overcoming the issues of small differences in thermodynamic stability, these methods produce the so-called on/off probes that can only differentiate one allelic variant (probe signal "on") from all other variants (probe signal "off") at a given polymorphic site.…”

Section: Synthesis and Intriguing Propertiesmentioning

confidence: 99%

Fluorescent silver nanoclusters as DNA probes

2013

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Fluorescent silver nanoclusters (few atoms, quantum sized) have attracted much attention as promising substitutes for conventional fluorophores. Due to their unique environmental sensitivities, new fluorescent probes have been developed based on silver nanoclusters for the sensitive and specific detection of DNA. In this review we present the recent discoveries of activatable and color-switchable properties of DNA-templated silver nanoclusters and discuss the strategies to use these new properties in DNA sensing.

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DNA Nanoswitch as a Biosensor

Cited by 24 publications

References 30 publications

DNA nanomachines and their functional evolution

DNA nanomachines and their functional evolution

Challenges of Pakistani Pharmaceutical Industry: Pakistan Case

Fluorescent silver nanoclusters as DNA probes

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