DNA of Epstein-Barr virus. III. Identification of restriction enzyme fragments that contain DNA sequences which differ among strains of Epstein-Barr virus

Raab‐Traub, Nancy; Pritchett, Randall; Kieff, Elliott

doi:10.1128/jvi.27.2.388-398.1978

Cited by 77 publications

(46 citation statements)

References 28 publications

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“…We have shown that the restriction pattern for the B95-8 cell-associated DNA and virion DNA are the same and are consistent with the pattern for B95-8 DNA as described in other studies (Raab-Traub et al, 1978). We have also attempted to rule out the possibility that the Raji cells in our laboratory are in some way more able to allow additional gene expression of the EBV genome, since we obtained similar results with Raji cells obtained from other laboratories.…”

Section: Discussionsupporting

confidence: 86%

“…The original report on B95-8 EBV (Miller et al, 1974) demonstrated that the virus was a transforming non-lytic strain of EBV; in that report unconcentrated cell supernates were used. Subsequent reports (e.g., Delius and Bornkamm, 1978;Fresen et al, 1978;Raab-Traub et al, 1978;Steinitz et al, 1978;Perlmann et al, 1982;Sairenji et al, 1982;Skare et al, 1985) make reference to this biological property of B95-8. Until recently (Takimoto et al, 1985;Sat0 et al, 1986) no EBV isolate other than HR-1 was shown to be able to lytically superinfect Raji cells.…”

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confidence: 99%

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The transforming prototype of epstein‐barr virus (B95‐8) is also a lytic virus

Glaser

Tarr

Dangel

1989

Intl Journal of Cancer

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The B95-8 isolate of the Epstein-Barr virus (EBV) has been described as a non-lytic transforming virus. We have performed experiments in order to determine if the B95-8 EBV is capable of super-infecting and replicating in EBV-genome-positive non-producer lymphoblastoid cells. Using concentrates of B95-8 EBV, prepared from 6 different B95-8 cell lines treated with 12-O-tetradecanoylphorbol-13-acetate (TPA), we demonstrated that virus concentrates could transform human or cotton-top tamarin B-lymphocytes and also lytically replicate in Raji cells, inducing EBV antigens and infectious virus. While the virus obtained from B95-8 super-infected Raji cells was able to transform cord-blood lymphocytes (CBLs) and super-infect Raji cells, transformation was abortive, with cell cultures only growing for up to 6 weeks. Transformation titers of the B95-8 virus concentrates ranged from 10(5) to greater than 10(8) transforming units/ml; early antigen (EA) induction ranged from 1% to 50% after superinfection of Raji cells, depending on the virus stock used, as determined by immunofluorescence. Southern blot analysis was carried out on the DNA prepared from B95-8 cells and virion DNA. The results were consistent with the published EcoRI restriction pattern for B95-8 EBV. The issue of whether the B95-8 cells produce virions with a dual biological phenotype or, rather, 2 biologically distinct viruses, is addressed.

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Section: Discussionsupporting

confidence: 86%

mentioning

confidence: 99%

The transforming prototype of epstein‐barr virus (B95‐8) is also a lytic virus

Glaser

Tarr

Dangel

1989

Intl Journal of Cancer

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“…A variable number of tandem direct repeats of a 3,000-bp sequence, IR, separates the largely unique DNA into a segment of 14 x 103 bp, US, and a segment of 135 x 103 bp, UL (6,7,14,15,20). The DNA of the B95-8 isolate differs from most EBV DNAs in a deletion from UL of 15 x 103 bp (5,7,21,40,41). Despite this deletion, the B95-8 virus, like other EBV isolates, can infect and growth transform normal B lymphocytes and replicate in some of the transformed cell lines (33,34).…”

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confidence: 99%

Epstein-Barr virus RNA. VIII. Viral RNA in permissively infected B95-8 cells

Hummel¹,

Kieff²

1982

J Virol

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More than 50 RNAs expressed by Epstein-Barr virus late in productive infection have been identified. B95-8-infected cells were induced to a relatively high level of permissive infection with the tumor promotor 12-O-tetradecanoylphorbol-13-acetate. Polyadenylated RNAs were extracted from the cell cytoplasm, separated by size on formaldehyde gels, transferred to nitrocellulose, and hybridized to labeled recombinant Epstein-Barr virus DNA fragments. Comparison of RNAs from induced cultures with RNAs from induced cultures also treated with phosphonoacetic acid to inhibit viral DNA synthesis identifies two RNA classes: a persistent early class of RNAs whose abundance is relatively resistant to viral DNA synthesis inhibition and a late class of RNAs whose abundance is relatively sensitive to viral DNA synthesis inhibition. The persistent early and late RNAs are not clustered but are intermixed and scattered through most of segments UL and US. The cytoplasmic polyadenylated RNAs expressed during latent infection were not detected in productively infected cells, indicating that different classes of viral RNA are associated with latent and productive infection. Non-polyadenylated small RNAs originally identified in cells latently infected with Epstein-Barr virus are expressed in greater abundance in productively infected cells and are part of the early RNA class.

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“…However, the latent protein LYDMA appears not to be expressed in all BL cell lines; several EBV-positive BL lines are not susceptible to HLAmatched LYDMA-specific killing (42). Another of the latent viral functions, EBNA-2, is apparently essential for transformation in that P3HR-1, a nontransforming subclone of the transforming BL Jijoye strain, is deleted for the sequences which encode EBNA-2 (25,40,41). Superinfection of the Raji cell line with HR-1 virus generates transformationcompetent recombinants which have regained the EBNA2 sequences (44).…”

Section: Discussionmentioning

confidence: 99%

Human neonatal lymphocytes immortalized after microinjection of Epstein-Barr virus DNA

Klein¹,

Raab‐Traub²

1987

J Virol

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Epstein-Barr virus (EBV) is a highly efficient acute transforming agent in human cells, provided that the intact virus is used. To investigate the ability of viral DNA alone to transform cells, we introduced the EBV genome into human lymphocytes. After microinjection of EBV DNA into neonatal B lymphocytes, we established a cell line that in early passages contained multiple viral fragments. This cell line retained sequences from the short, unique (U,) region of the EBV genome and sequences from EcoRI-E. The viral sequences were not expressed; however, the cells expressed a 2.3-kilobase polyadenylated message homologous to the c-fgr oncogene, a cellular locus believed to be activated by EBV infection [M.

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DNA of Epstein-Barr virus. III. Identification of restriction enzyme fragments that contain DNA sequences which differ among strains of Epstein-Barr virus

Cited by 77 publications

References 28 publications

The transforming prototype of epstein‐barr virus (B95‐8) is also a lytic virus

The transforming prototype of epstein‐barr virus (B95‐8) is also a lytic virus

Epstein-Barr virus RNA. VIII. Viral RNA in permissively infected B95-8 cells

Human neonatal lymphocytes immortalized after microinjection of Epstein-Barr virus DNA

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