DNA photoproducts released by repair in biological fluids as biomarkers of the genotoxicity of UV radiation

Abstract

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Nuclear and Urinary Measurements Show the Efficacy of Sun-Protection Factor 50+ Sunscreen against DNA Photoproducts upon Real-Life Recreational Exposure

“…Photoproducts arising from DNA repair could be unambiguously detected in urine after exposure in both control and educated groups. A recently developed method ( Reynaud et al., 2022 ) relies on the isolation and enzymatic hydrolysis of the oligonucleotides released by the DNA-repair machinery. In that respect, it is more quantitative than the previous urinary measurements based on 32 P post-labeling ( Kotova et al., 2005 ; Le Curieux and Hemminki, 2001 ; Petersen et al., 2014 ) or a recent HPLC-MS/MS method ( Lerche et al., 2022 ).…”

“…This approach is efficient but invasive. An assay was recently developed for the quantification of the photoproducts released in biological fluids by the DNA-repair machinery ( Reynaud et al., 2022 ). In this study, it was applied to samples collected from volunteers after a period of summer vacation.…”

“…Urinary photoproducts (TT CPDs and TT 64PP) released upon DNA repair were quantified using a recently reported assay ( Reynaud et al., 2022 ). Briefly, triethylammonium acetate was added to 100 μl of urine, together with a known amount of oligonucleotides bearing ethenoadenine used as the internal standard for the overall recovery.…”

“…This was performed on the roof of suction blisters, an approach that is less invasive for the skin and the donor than classical biopsies ( Josse et al., 2020 , 2018 ). The second and innovative method was the quantification of the pyrimidine dimers released in urine by the DNA-repair machinery ( Reynaud et al., 2022 ). This study, to our knowledge, shows a previously unreported application of this noninvasive strategy to the assessment of photoprotection.…”