DNA ploidy heterogeneity in early and advanced gastric cancers

Fujimaki, Eiji; Sasaki, Kohsuke; Nakano, Osamu; Chiba, Shigeki; Tazawa, Hideki; Yamashiki, Hiromasa; Orii, Seishi; Sugai, Tamotsu

doi:10.1002/(sici)1097-0320(19960615)26:2<131::aid-cyto6>3.3.co;2-n

Cited by 4 publications

(8 citation statements)

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“…In a purely diploid early cancer genetic instability leads to a more aggressive (purportedly more invasive) cell clone, which is reflected in DNA-ploidy heterogeneity. Overgrowth by this clone of the tumour eventually leads to homogeneous aneuploidy [4,6,17,22].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Heterogeneity of DNA Ploidy in Early Gastric Cancers

Osterheld

Laurini

Saraga

et al. 1999

Analytical Cellular Pathology

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DNA ploidy has been shown to be a predictive parameter for prognosis in various solid tumours. The prognostic value of DNA‐ploidy in gastric cancers is still a matter of controversy. A possible explanation for the discrepant results reported in the literature could be sampling error in tumours with multiple stemlines differing in DNA‐ploidy. In order to determine whether or not such heterogeneity exists in early gastric carcinoma, we have performed DNA cytophotometry on multiple samples of a group of 17 early gastric carcinomas, of which 8 were pure intramucosal and 9 were infiltrating into the submucosa. We found an aneuploid DNA‐stemline in 8 (47%) early gastric cancers, more often in tumours invading into the submucosa (5/9) than in purely mucosal tumours (3/8). Multiple DNA‐stemlines were found more frequently in submucosally infiltrating tumours (4/5). These results confirm the presence of DNA‐aneuploid early gastric carcinoma which are frequently heterogeneous and suggest that heterogeneity occurs more frequently in tumours invading the submucosa. This heterogeneity is best detected by analysing multiple samples of tumours for DNA‐ploidy.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Heterogeneity of DNA Ploidy in Early Gastric Cancers

Osterheld

Laurini

Saraga

et al. 1999

Analytical Cellular Pathology

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“…However, multiple subclones are found in advanced diffuse‐type cancers. This seems to suggest that tumor cells of diffuse‐type cancers are more genetically stable than those of intestinal‐type cancers during the early stage, as supported by the high frequency of DNA diploidy in diffuse‐type cancers (7, 8).…”

Section: Discussionmentioning

confidence: 94%

“…Thus, DNA ploidy heterogeneity can exist within the same tumor (6, 7). This is common among gastric cancers (8), as supported by the finding that different histological types coexist within the same gastric cancer. DNA flow cytometric analysis can quantitatively and rapidly analyze a large population of tumor cells, although recently developed molecular genetic approaches have begun to provide an alternative powerful tool to detect the molecular mechanisms involved in cancer (1).…”

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confidence: 79%

“…Diffuse‐type cancers of the stomach are reportedly primarily diploid in intramucosal cancers and often aneuploid in advanced stages (7, 8), whereas intestinal‐type cancers tend to be aneuploid at both the early and advanced stages (7). These findings indicate that although DNA aneuploidy occurs during the early phase of intestinal‐type cancers (intramucosal cancer), this is generally a late event in diffuse‐type cancers (7).…”

Section: Discussionmentioning

confidence: 99%

“…Intratumoral DNA differences are frequently found in a variety of human cancers (6, 8, 16, 17). Among intestinal‐type cancers, multiple subclones are seen in submucosal cancers as well as intramucosal cancers.…”

Section: Discussionmentioning

confidence: 99%

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DNA mapping of gastric cancers using flow cytometric analysis

et al. 2000

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Although numerous studies of gastric cancers on DNA ploidy have been reported, differences in the degree of aneuploidy (DNA index, DI) during progression have not been identified. We attempted to chart the differences in DIs during progression to clarify the role of aneuploidy in gastric cancers. We classified the gastric cancers examined into intestinal (n = 88) and diffuse (n = 48) types, and then analyzed 136 gastric cancers (intramucosal cancer, 42; submucosal cancer, 39; advanced cancer, 55) by flow cytometry using multiple sampling. In addition, we examined the DNA ploidy pattern of mucosal and submucosal lesions using the same submucosal cancers to study the tumor progression in individual cancers. Intratumoral DNA differences in DNA ploidy were observed in both types of gastric cancers. In intestinal‐type cancers, multiple subclones indicated by a different DI occurred during the early stage of gastric cancers, whereas in diffuse‐type cancers, multiple subclones were found primarily in advanced cancers. Although the DI varied widely in early intestinal‐type cancers between 1.0 and 2.0, in early diffuse‐type cancers, the DI tended to be less than 1.2. However, in advanced stage gastric cancers, the DI distribution was similar for both histological types. In intestinal‐type cancers, high DI (>1.3) aneuploidy was frequently found in mucosal lesions. In contrast, only low DI (<1.2) aneuploid clones were observed in mucosal lesions of diffuse‐type cancers. The present results suggest that high DI aneuploid tumor clones in intramucosal cancers acquire invasive ability when they progress to submucosal cancers, whereas DNA aneuploidy itself plays an important role in submucosal invasion of diffuse‐type cancers. Cytometry (Comm. Clin. Cytometry) 42:270–276, 2000. © 2000 Wiley‐Liss, Inc.

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