DNA Ploidy, S Phase Fraction and G2 Fraction as
Prognostic Determinants in Prostatic Adenocarcinoma

Eskelinen, Matti; Lipponen, P; Majapuro, R; Syrjänen, Karí; Nordling, Stig

doi:10.1159/000471663

Cited by 16 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…35 However, a determination of Sphase fraction was not found to contribute additional prognostic information in a multivariate model restricted to locally confined clear cell RCC. of studies supporting both sides of this issue.…”

Section: Discussionmentioning

confidence: 91%

Appraisal of intratumoral microvessel density, MIB-1 score, DNA content, and p53 protein expression as prognostic indicators in patients with locally confined renal cell carcinoma

Gelb

Sudilovsky

et al. 1997

Cancer

View full text Add to dashboard Cite

kidney (classified as T1N0M0 or T2N0M0) were analyzed for iMVD, MIB-1 score, DNA content, S-phase fraction, and p53 protein expression by immunohistochemi- used as a marker for the tumor cell proliferation rate. DNA content and S-phase 3 Division of Pathology, City of Hope National fraction were determined by flow cytometry using paraffin embedded tissue. p53 Medical Center, Duarte, California.expression was assessed using the D07 antibody. RESULTS.The median time of clinical follow-up was ú 9 years. Eleven patients died of disease; the median time to death was 26 months. iMVD counts using antifactor VIII and anti-CD31 were tightly correlated (correlation coefficient Å 0.89). S-phase fraction was higher in aneuploid tumors than in diploid tumors (mean, 12.4% vs. 4.3%; P Å 0.01). Using univariate survival analyses, tumor size (stage classification pT1 vs. PT2; P Å 0.01) and nuclear grade (P Å 0.04) were associated with shortened survival. No statistically significant differences in survival were found for iMVD, MIB-1 score, DNA content, S-phase fraction, or p53 expression. Only two cases strongly expressed p53 protein; both tumors were of Presented in part at the United States and Canahigh nuclear grade. Using multivariate survival analyses, nuclear grade and tumor dian Academy of Pathology Annual Meeting, Orsize were the only independent prognostic factors (best model P Å 0.002). lando, Florida, March 1-7, 1997. CONCLUSIONS.In this study, nuclear grade and tumor size were found to be independent predictors of survival in locally confined clear cell (conventional) RCC, The authors thank Dr. Peter Bacchetti for his advice concerning biostatistics, and Karen Feras has been shown previously for locally confined RCC in general. MIB-1 score, reira, Radha Dutta-Roy, and Taya Berry for their iMVD counts, DNA content, S-phase fraction, and p53 expression did not contribhelp with the DNA content (ploidy) studies.ute additional prognostic information.

show abstract

Section: Discussionmentioning

confidence: 91%

Appraisal of intratumoral microvessel density, MIB-1 score, DNA content, and p53 protein expression as prognostic indicators in patients with locally confined renal cell carcinoma

Gelb

Sudilovsky

et al. 1997

Cancer

View full text Add to dashboard Cite

show abstract

“…The increase in AR in low-grade tumors as measured by immunohistochemistry is not quantitative and is a better measure of percentage 29 rather than intensity of staining. The fact that AR density is related to S-phase fraction, which has been shown in prior studies [32][33][34][35][36] to be associated with poor prognostic features and outcome, may indicate that the significance of AR density may be different from that of the percentage of AR-positive cells. For example, the amount of ARs per cell may be increased due to the presence of nonfunctioning AR with upregulation, a mutation leading to sensitivity of AR to other substrates or simply increased AR due to androgenic stimulation leading to more division.…”

Section: Discussionmentioning

confidence: 99%

“…22 The additional advantage to this technique is the ability to measure S-phase fraction and ploidy , which have been found to have prognostic value in several publications. [32][33][34][35][36] ARs may stimulate proliferation through their mechanism of action. However, it is not known whether high AR density correlates with a higher S-phase fraction, with its adverse prognostic implications.…”

Section: Introductionmentioning

confidence: 99%

Androgen receptor antigen density and S-phase fraction in prostate cancer: a pilot study

Abdel‐Wahab

Krishan

Milikowski

et al. 2003

Prostate Cancer Prostatic Dis

View full text Add to dashboard Cite

Purpose: To determine whether quantitative flow cytometric androgen receptor density expression (MFC ratio) in prostate cancer was associated with S-phase fraction. Methods: Flow cytometry was performed to determine DNA aneuploidy, S-phase fraction, percentage of androgen receptor (AR)-positive cells, and MFC ratio in prostate cancer patients. Results: MFC ratio showed distinct clustering. Eight patients had a low MFC ratio of 1.78-2.74, while 10 patients had high MFC ratios between 4.99 and 6.48. The Sphase fraction had average values of 11.05 vs 4.92 in tumors with high vs low MFC ratio (Po0.01). Conclusion: S-phase fraction was significantly higher in tumors with high AR density.

show abstract

“…9 level 3b studies report the same after radical prostatectomy [167,170,184, • 5 level 2b studies [170,180,182,190,225] report a significant correlation between DNA-ploidy parameters and the occurrence of local progression or recurrence after radical prostatectomy, 1 after hormonal therapy [234], 1 after brachytherapy [227] ( Table 7).…”

Section: Methodologically Sufficient Papersmentioning

confidence: 99%

DNA-cytometric grading of prostate cancer Systematic review with descriptive data analysis

Böcking¹,

Tils²,

Schramm³

et al. 2014

Pathol Discov

View full text Add to dashboard Cite

Gleason-score <=6, assessed on core needle biopsies, is an essential prognostic parameter to offer the strategy of Active Surveillance (AS) to patients with locally confined cancers of the prostate. Yet, its interobserver reproducibility is low (48-70%) and its prognostic validity unsatisfactory. An option to complementary assess the malignant potential of these cancers are objective DNA-ploidy-measurements on existing biopsies. For that purpose chromosomal heterogeneity is indirectly quantified by DNA-cytometry resulting in DNA-grades of malignancy 1-4. This review systematically trawls and evaluates all scientific publications on the potential diagnostic and prognostic validity and the heterogeneity of DNA-ploidy measurements in cancers of the prostate between 1966 and 2013. Publications have been classified into Oxford levels of evidence and levels of significance were given for the correlation of DNA-ploidy with different clinical outcomes. 114 scientific articles had to be excluded because of different methodological reasons. All but one of the 67 methodologically acceptable articles report on a significant diagnostic resp. prognostic significance of DNA ploidy measurements in cancers of the prostate. 8 level 1b studies report that DNA-ploidy, assessed on punch biopsies independently predicts organ confinement as assessed after radical prostatectomy. 18 level 2b studies prove that DNA-ploidy measurements add statistically significant information to the Gleason-score. 16 level 2b investigations report a significant correlation of DNA-ploidy with recurrence-free survival. 15 level 2b studies document a significant correlation with overall survival after different types of therapy. 5 level 2b investigations prove a significant correlation with local recurrence or progress after radical prostatectomy. 3 level 2b publications show a significant correlation of DNAploidy with the occurrence of lymph node-or bone metastases after radical prostatectomy. 1 level 2b study documents the additional prognostic value of DNA-ploidy measurements over conventional subjective grading in prostate cancer patients under AS. All existing 15 narrative reviews on selected articles dealing with prognostic DNA-cytometry in cancers of the prostate are in favor of this method. Prospective level 1b studies, especially those proving the validity of DNA ploidy measurements to predict non-progression in patients with clinically insignificant low-grade low stage cancers of the prostate eligible for Active Surveillance additionally to the Gleason-score are still missing.

show abstract

DNA Ploidy, S Phase Fraction and G2 Fraction as Prognostic Determinants in Prostatic Adenocarcinoma

Cited by 16 publications

References 15 publications

Appraisal of intratumoral microvessel density, MIB-1 score, DNA content, and p53 protein expression as prognostic indicators in patients with locally confined renal cell carcinoma

Appraisal of intratumoral microvessel density, MIB-1 score, DNA content, and p53 protein expression as prognostic indicators in patients with locally confined renal cell carcinoma

Androgen receptor antigen density and S-phase fraction in prostate cancer: a pilot study

DNA-cytometric grading of prostate cancer Systematic review with descriptive data analysis

Contact Info

Product

Resources

About