DNA Probes and Primers in Dental Practice

Conrads, Georg

doi:10.1086/341924

Cited by 23 publications

(19 citation statements)

References 30 publications

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“…F. nucleatum subsp. polymorphum represents a separate phylogenetic branch which includes several human pathogens (Conrads, 2002;Gmur et al, 2006;Goldstein et al, 1995). At a species level, its metabolic versatility enables it to create and survive conditions conducive to the establishment of pathogenic obligate anaerobes (Diaz et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Effect of alkaline growth pH on the expression of cell envelope proteins in Fusobacterium nucleatum

Zilm

Mira²,

Bagley

et al. 2010

Microbiology

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Fusobacterium nucleatum is a Gram-negative anaerobic organism that plays a central role in the development of periodontal diseases. The progression of periodontitis is associated with a rise in pH of the gingival sulcus which promotes the growth and expression of virulence factors by periodontopathic bacteria. We have previously reported that the expression of specific cytoplasmic proteins is altered by a shift in growth pH. In the present study we have compared cell envelope protein expression of F. nucleatum during chemostat growth at pH 7.2 and 7.8. From a total of 176 proteins resolved from the cell envelope, 15 were found to have altered expression in response to an increase in growth pH and were identified by MS. Upregulated proteins included an outer membrane porin which has been identified as playing a role in virulence, a periplasmic chaperone which assists in the folding of outer membrane proteins, and a transporter thought to be involved with iron uptake. Proteins downregulated at pH 7.8 were consistent with our previous findings that the bacterium reduces its catabolism of energy-yielding substrates in favour of energy-storage pathways. Among the downregulated proteins, two transporters which are involved in the uptake of C4 dicarboxylates and phosphate were identified. A putative protease and an enzyme associated with the metabolism of glutamate were also identified. A high proportion of the cell envelope proteins suggested by these data to play a role in the organism's response to alkaline growth pH may have arisen by lateral gene transfer. This would support the hypothesis that genes that provide an ability to adapt to the changing conditions of the oral environment may be readily shared between oral bacteria.

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Section: Discussionmentioning

confidence: 99%

Effect of alkaline growth pH on the expression of cell envelope proteins in Fusobacterium nucleatum

Zilm

Mira²,

Bagley

et al. 2010

Microbiology

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“…Studies have determined their relative prevalences, interactions and virulence factors [2-7]. By the end of the 1980's, the development of novel, culture-independent techniques allowed the identification of as-yet-unculturable and fastidious organisms in patients suffering from periodontitis and added new insight into bacterial communities in periodontal pockets [8-10]. In recent years, research has detected increasing numbers of bacterial species and phylotypes in subgingival plaque and other habitats of the human oral cavity [11-18].…”

Section: Introductionmentioning

confidence: 99%

Filifactor alocis - involvement in periodontal biofilms

et al. 2010

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BackgroundBacteria in periodontal pockets develop complex sessile communities that attach to the tooth surface. These highly dynamic microfloral environments challenge both clinicians and researchers alike. The exploration of structural organisation and bacterial interactions within these biofilms is critically important for a thorough understanding of periodontal disease. In recent years, Filifactor alocis, a fastidious, Gram-positive, obligately anaerobic rod was repeatedly identified in periodontal lesions using DNA-based methods. It has been suggested to be a marker for periodontal deterioration. The present study investigated the epidemiology of F. alocis in periodontal pockets and analysed the spatial arrangement and architectural role of the organism in in vivo grown subgingival biofilms.ResultsA species-specific oligonucleotide probe, FIAL, was designed and evaluated. A total of 490 subgingival plaque samples were submitted to PCR and subsequent dot blot hybridization to compare the prevalence of F. alocis in patients suffering from generalized aggressive periodontitis (GAP), chronic periodontitis (CP), and control subjects resistant to periodontitis. Moreover, a specially designed carrier system was used to collect in vivo grown subgingival biofilms from GAP patients. Subsequent topographic analysis was performed using fluorescence in situ hybridization.While the majority of patients suffering from GAP or CP harboured F. alocis, it was rarely detected in the control group. In the examined carrier-borne biofilms the organism predominantly colonized apical parts of the pocket in close proximity to the soft tissues and was involved in numerous structures that constitute characteristic architectural features of subgingival periodontal biofilms.ConclusionsF. alocis is likely to make a relevant contribution to the pathogenetic structure of biofilms accounting for periodontal inflammation and can be considered an excellent marker organism for periodontal disease.

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“…In jedem Fall sollte während der Probenentnahme kein übermäßiges Bluten der Zahnfleischtasche vorliegen, da die üblicherweise verwendeten Papierspitzen nur etwa 20 µl Flüssigkeit aufnehmen können [5]. …”

Section: Probenentnahme Und Reevaluationunclassified