DNA purification and concentration by isotachophoresis in nonwoven fabric strip

Duša, Filip; Moravcová, Dana; Šlais, Karel

doi:10.1016/j.aca.2020.04.029

Cited by 3 publications

(1 citation statement)

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“…Large DNA (>50 kb) breaks in these systems due to the fragility of large molecules. Other methods to concentrate DNA were isotachophoresis in a nonwoven fabric strip (200–2000 bp) [19], a fabricated chip device (48.5 kb) [20], and pressure‐driven flow with parallel electric field (48.5 kb) [21]. Identifying large variations requires larger DNA molecules and using systems that can only concentrate <50 kb will not work for Nanocoding.…”

Section: Introductionmentioning

confidence: 99%

Concentration of lambda concatemers using a 3D printed device

et al. 2023

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Identifying significant variations in genomes can be cumbersome, as the variations span a multitude of base pairs and can make genome assembly difficult. However, large DNA molecules that span the variation aid in assembly. Due to the DNA molecule's large size, routine molecular biology techniques can break DNA. Therefore, a method is required to concentrate large DNA. A bis‐acrylamide roadblock was cured in a proof‐of‐principle 3D printed device to concentrate DNA at the interface between the roadblock and solution. Lambda concatemer DNA was stained with YOYO‐1 and loaded into the 3D printed device. A dynamic range of voltages and acrylamide concentrations were tested to determine how much DNA was concentrated and recovered. The fluorescence of the original solution and the concentrated solution was measured, the recovery was 37% of the original sample, and the volume decreased by a factor of 3 of the original volume.

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Section: Introductionmentioning

confidence: 99%