DNA Radiolysis by Fast Neutrons

Spotheim-Maurizot, M.; Charlier, Michel; Sabattier, R.

doi:10.1080/09553009014552421

Cited by 102 publications

(35 citation statements)

References 33 publications

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“…où S et L représentent respectivement les fractions (%) détectables de molécules dans l'état topologique surenroulé et linéaire de l'ADN plasmidique [14,15].…”

Section: Materiel Et Methodes Dispositif D'irradiation Et Dosimetrieunclassified

Complexation des acides aminés basiques arginine, histidine et lysine avec l’ADN plasmidique en solution aqueuse : participation à la capture de radicaux sous irradiation X à 1,5 keV

et al. 2016

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“…où S et L représentent respectivement les fractions (%) détectables de molécules dans l'état topologique surenroulé et linéaire de l'ADN plasmidique [14,15].…”

Section: Materiel Et Methodes Dispositif D'irradiation Et Dosimetrieunclassified

Complexation des acides aminés basiques arginine, histidine et lysine avec l’ADN plasmidique en solution aqueuse : participation à la capture de radicaux sous irradiation X à 1,5 keV

et al. 2016

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“…In Fig. 2a we show the results from reference [6] for gamma and neutron irradiations, and in Fig. 2b we present our first results for proton and gamma.…”

Section: Experimental Verificationmentioning

confidence: 96%

Radiation interaction with DNA

Deppman¹,

Echeimberg²,

Gouveia³

et al. 2004

Braz. J. Phys.

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We developed a model to describe the radiation-DNA interaction in water solution. The model is based on some important aspects of the Bethe Theory for the ionizing process, and on the assumption that the direct interaction between the incident particle and the DNA molecule is negligible in comparison with the indirect effect. This assumption can always be true under controlled conditions. The results show that the number of damages induced by radiation depends on the accumulated dose, on parameters related only to the medium properties, and on the DNA structure, but not on the incident particle. A few applications of the model are discussed, and results are successfully compared with experimental data. New experiments are proposed.

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“…This translates to the activity and proportion of esterase, hence the change of esterase-isoenzyme spectrum can signal the change of thermo-tolerance ability to, at least, some magnitude. Since esterase isoenzymes are coded by nuclear genes, the Furthermore, according to the percentage of linear and supercoil forms of DNA, the radiation chemical yield G-values for DSB and SSB formation can be calculated as described by Spotheim-Maurizot et al (1990) and Klimczak et al (1993). The relationaship between doses and DSB or SSB formation fits the equation: y = kD + b, and the results were that K SSB = 9.1 × 10 -4 Gy -1 K DSB = 7.2 x 10 -4 Gy -1 , then the G-value was obtained by the equation of G = k/w (W: molecular weight of plasmid, 2.86 x 10 6 for pBR322).…”

Section: Esterase Isoenzymes Variety Of Mutated Yeast Strainmentioning

confidence: 99%

Mutation breeding for productive yeast strains through a novel method: High-energy-pulse-electron-beam

Hong¹,

Xu²,

Shi³

et al. 2008

Ann. Microbiol.

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INTRODUCTIONThe discovery that X-rays induce mutations in fruit fly Drosophila melanogaster (Muller, 1927) and in barley (Stadler, 1928) has initiated a new field-radiation induced mutagenesis (Ahloowalia et al., 2004). Till today, many mutation-induction methods, including -rays, -rays, fast neutrons, ultraviolet (UV) radiation, laser beam, low energy ion beam, and space breeding, have been widely studied and applied in radiation-induced mutational breeding. During the past 80 years, more than 3500 new-species worldwide have been created and released, according to the statistic data by International Atomic Energy Agency. Many welladapted and high-yielding varieties of plant and microorganism such as wheat, cotton, rice, rapeseed, sunflower, sesame, grapefruit, banana and industrial yeast and bacteria strains have been selected and cultivated through radiation, bringing huge economic and social benefits to our society.Though mutation induction has long been used as one of the important methods for species improvement, the efficiency of mutation induction and selection of current methods is still low and ineffective, requiring screening of large population for low-rate mutants which is laborious and costly (Brunner, 1995). New effective methods with higher mutation efficiency are extremely desired. High-energy-pulse-electron beam (HEPE) was mainly used in pulse radiolysis and material surface modification in the past. There has been no report about the application of HEPE in mutation creation. The HEPE equipment used in present research has exceptional high energy capacity and absorbed-dose rate (absorbed dose per unit time), with a 5 millimetres diameter beam of 4 ampere pulse current in 2 nano-seconds. The maximum absorbed dose-rate in water measured by potassium thiocyanate dosimeter may reach 10 10 Gys -1 , which is much higher than that of -rays (usually under 60 Gys -1 ) and those of other radiation methods.Many experiments have demonstrated that dose rate exhibits strong influence on relative biological effectiveness. This type of relationship is called a normal dose-rate effect (Hoglund et al., 2000). Compared with ordinary electron beam and other radiation methods, HEPE possesses much higher dose rate and stronger penetrating ability, is capable of inducing more DNA lesions and mutations. According to the typical breeding theory, high density DNA damage is difficult to be, or cannot be, repaired completely, causing more un-repaired DNA damage, hence the base of mutation breeding. HEPE has great potential in radiation-induced mutation breeding.On the other hand, the discovery that space-flight breeding has much higher breeding efficiency than other radiation methods (Ermolenko et al., 2000) sheds lights on the theory of radiation breeding. It has been speculated that there are many high energy particles and waves with much higher doserate-effects in outer space. With high dose-rate, HEPE is partial simulation of outer-space-radiation environment.In this work, we have applied HEPE to yeast mutation inductio...

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DNA Radiolysis by Fast Neutrons

Cited by 102 publications

References 33 publications

Complexation des acides aminés basiques arginine, histidine et lysine avec l’ADN plasmidique en solution aqueuse : participation à la capture de radicaux sous irradiation X à 1,5 keV

Complexation des acides aminés basiques arginine, histidine et lysine avec l’ADN plasmidique en solution aqueuse : participation à la capture de radicaux sous irradiation X à 1,5 keV

Radiation interaction with DNA

Mutation breeding for productive yeast strains through a novel method: High-energy-pulse-electron-beam

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