DNA rearrangement on the octadecylamine modified graphite surface by heating and ultrasonic treatment

Abstract

The evolution of single-stranded DNA (ssDNA) assembly on octadecylamine (ODA) modified highly oriented pyrolytic graphite (HOPG) surface by heating and ultrasonic treatment has been studied for the first time. We have observed that DNA on the ODA coated HOPG surface underwent dramatic morphological changes as a function of heating and ultrasonic treatment. Ordered DNA firstly changed to random aggregates by heating and then changed to three-dimensional (3D) networks by ultrasonic treatment. This finding points to previously unknown factors that impact graphite–DNA interaction and opens new opportunities to control the deposition of DNA onto graphitic substrates. In this way, we built a cost-effective method to produce large-scale 3D ssDNA networks. All of these studies pave the way to understand the properties of DNA–solid interface, design novel nanomaterials, and improve the sensitivity of DNA biosensors.

Ordered Assembly of DNA on Topological Insulator Bi2Se3 and Octadecylamine for a Sensitive Biosensor

“…These ordered templates guided the DNA molecules on the HOPG surface . We first deposited ODA onto Bi 2 Se 3 by the reported method . That is, evaporation of ODA powder on a hot plate in the fume hood in the air at 100 °C for 1 min.…”

“…39 We first deposited ODA onto Bi 2 Se 3 by the reported method. 40 That is, evaporation of ODA powder on a hot plate in the fume hood in the air at 100 °C for 1 min. The dot layer was formed on the Bi 2 Se 3 surface with a height of 1−2 nm (Figure 3d).…”

“…The electrostatic interactions between the phosphate of DNA and oppositely charged head groups of the ODA layer become predominant and drive the assembly of the DNA molecules . However, the commonly used method of evaporation ODA in a fume hood − is easy to form globule structures. For the first time, we coat the ODA layer by means of chemical vapor deposition in argon.…”