DNA recombination associated with short direct repeats in Leishmania mexicana M379

Liu, Jianhua; Salinas, Graciela; Gajendran, Nadesan; Muthui, David; Hamers, R.

doi:10.1016/0166-6851(92)90233-a

Cited by 23 publications

(8 citation statements)

References 13 publications

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“…Amplicons are formed at the level of homologous sequences usually ranging from 200 to 1,200 bp in Leishmania tarentolae (14,29), although smaller homologous sequences have also been proposed to be involved in amplicon formation in Leishmania spp. (20,21) and even other mechanisms (26), yet to be defined precisely, may also account for amplicon formation. There is apparently little, if any, need for homologous sequences for the rearrangement of chromosomal sequences to yield circular amplicons in tumor cells (13,40).…”

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confidence: 99%

Formation of Extrachromosomal Circular Amplicons with Direct or Inverted Duplications in Drug-Resistant Leishmania tarentolae

Grondin

Roy

Ouellette

1996

Molecular and Cellular Biology

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Selection for methotrexate resistance in Leishmania spp. is often associated with amplification of the H locus short-chain dehydrogenase-reductase gene ptr1 as part of extrachromosomal elements. Extensive sequences are always coamplified and often contain inverted duplications, most likely formed by the annealing of inverted repeats present at the H locus. By gene targeting mediated by homologous recombination, several repeated sequences were introduced in the vicinity of ptr1. Selection for methotrexate resistance in these transfectants led to ptr1 amplification as part of small circles with direct or inverted duplications whether the integrated sequences consisted of direct or inverted repeats. Hence, for a region to be amplified in L. tarentolae during drug selection, a drug resistance gene is required and must be flanked by (any) homologous repeated sequences. The distance between these repeats and their orientation will determine the length of the amplicon and whether it contains direct or inverted duplications.

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confidence: 99%

Formation of Extrachromosomal Circular Amplicons with Direct or Inverted Duplications in Drug-Resistant Leishmania tarentolae

Grondin

Roy

Ouellette

1996

Molecular and Cellular Biology

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“…In phage k, the chi sequence 5¢-GCTGGTGG-3¢ functions as a recombination hotspot, with recombination occurring when the RecBCD enzyme induces a nick on the DNA strand bearing the chi sequence (Thaler and Stahl 1988). Chi-like sequences occur in Leishmania mexicana M379 and Trypanosoma, some in association with tandemly repeated genes (Le Blancq et al 1988;Kendall et al 1990;Liu et al 1992). …”

Section: Resultsmentioning

confidence: 99%

Repetitive sequences in the ITS1 region of ribosomal DNA in congeneric microphallid species (Trematoda: Digenea)

2005

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In searching for species-specific DNA sequences of microphallid species (Digenea, Trematoda) we examined the ribosomal internal transcribed spacer regions (ITS) of three closely related species (Levinseniella group) hosted by mud snails (first intermediate host) and marine crustaceans (second intermediate host). In the ITS1 region we found consistent patterns of repeating sequences of 130 bp. Within each main repeat there was a varying number of subrepeats specific for each of the species. All repeats including subrepeats were identified by a similar starting sequence: 5'-CCTGTGG-3'. As this sequence has close resemblance to the chi sequence 5'-GCTGGTGG-3' found in phage lambda we speculate if it serves the same function as a recombination hotspot. Alternatively but less likely, it could be an inactive, mutational relic of a sequence that once served this purpose.

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“…Therefore, dermatologists are mostly concerned with the L. tropica, L. mexicana, and L. braziliensis complexs. Some of the base sequences of their kinetoplast DNA have already been reported (2,7).…”

Section: Discussionmentioning

confidence: 99%

A Preliminary Study Aimed at the Detection of Leishmania Parasites in Subjects with Cutaneous Leishmaniasis Using Polymerase Chain Reaction

Uezato

Hagiwara

Hosokawa

et al. 1998

The Journal of Dermatology

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As a basic study for future diagnosis of cutaneous leishmaniasis, we tried to detect Leishmania parasites representing different species in the subgenera Leishmania and Viannia from subject patients with cutaneous leishmaniasis by using the polymerase chain reaction (PCR) with the subgenus Viannia specific primer. Four out of the 14 specimens revealed an amplified DNA of 70 bp specific for the subgenus Viannia (L. braziliensis complex). No bands were detected in the rest of the specimens belonging to the subgenus Leishmania and unclassified groups. The base sequences of the amplified DNA corresponded with those of the L. (V). braziliensis kinetoplast minicircle. We concluded that PCR using the present primer specific for the subgenus Viannia would be useful in detecting Leishmania parasites in lesions of cutaneous leishmaniasis caused by the L. braziliensis complex.

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DNA recombination associated with short direct repeats in Leishmania mexicana M379

Cited by 23 publications

References 13 publications

Formation of Extrachromosomal Circular Amplicons with Direct or Inverted Duplications in Drug-Resistant Leishmania tarentolae

Formation of Extrachromosomal Circular Amplicons with Direct or Inverted Duplications in Drug-Resistant Leishmania tarentolae

Repetitive sequences in the ITS1 region of ribosomal DNA in congeneric microphallid species (Trematoda: Digenea)

A Preliminary Study Aimed at the Detection of Leishmania Parasites in Subjects with Cutaneous Leishmaniasis Using Polymerase Chain Reaction

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