DNA recovery from different evidences in 300 cases of sexual assault

Bozzo, W.R.; Colussi, A.; Ortiz, M.; Lojo, María Mercedes

doi:10.1016/j.fsigss.2009.08.185

Cited by 10 publications

(7 citation statements)

References 1 publication

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“…The 1.875 % ranking of the Fibre evidence shows that the evidence is less available and may not be the key while collecting evidence. Semen is good evidence [38][39][40], but physical barriers used as protection make it less available evidence. It may be one of the thoughts of the professionals who did the ranking and possibly among other related professionals.…”

Section: Discussionmentioning

confidence: 99%

Medical laboratory sciences and lectures a veritable tool in forensic science: A comparative review study of rape in Nigeria.

Nwachukwu

2024

JPAM

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Section: Discussionmentioning

confidence: 99%

Medical laboratory sciences and lectures a veritable tool in forensic science: A comparative review study of rape in Nigeria.

Nwachukwu

2024

JPAM

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“…Such biological items are referred to as evidentiary exhibits [25,26]. Out of these evidentiary items, semen samples are considered the most signi icant exhibits to prove sexual contact and to identify an accused through DNA ingerprinting [27][28][29][30].…”

Section: Types Of Biological Evidentiary Items/exhibits In Sexual Assault Casementioning

confidence: 99%

Application and utility of alternative methods in isolation of pure cells from forensic biological mixtures in modern-day: a review

Prajapati¹,

Rajmane²,

Jayakrishna³

et al. 2021

J Forensic Sci Res

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Development of genetic profiles from the biological mixtures has remained challenging, although modern-day technologies may help forensic scientists to attain a reliable genetic profile in the identification of the accused. In the case of rape, vaginal swab exhibits usually contain epithelial cells of victims and sperm cells of accused, such samples are more challenging when there is more than one contributor. In such cases, separation of distinct cells from a mixture that includes blood cells, epithelial cells and sperm cells for their single genetic profile is important. In the last ten decades several new techniques were developed and invented for the separation of single cell from the biological mixture that includes differential lysis, laser micro-dissection, cell sorting (FACS), sieve-based filtration, (vi) micro-fluidic devices or immunomagnetic beads cell separation of fresh samples, and the magnetic activated cell sorting (MACS). Out of them, some techniques have been commonly applied for cell separation in forensic biology. Each technique has its own limitation. Some recent studies showed, magnetic activated cell sorting (MACS), laser capture microdissection (LCM), DEPArray technology and fluorescence activated cell sorting (FACS) has proved to be effective in separation of single cell from cell mixtures. Therefore, in this review we have evaluated these four alternative methods and their potential application in the modern-day over the others for the separation of a single cell from the mixture. In this review we also discuss the advantage of these methods and their modern–day applicability and acceptance in the forensic world.

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“…Evidence may be found at the victim's body or clothes, in condoms or bed clothes, or at the crime scene [ 2 , 11 , 16 – 19 ]. Therefore, the examiner should rapidly inform police to isolate and protect the crime scene and should collect first the more urgent samples [ 20 ].…”

Section: Forensic Evidencementioning

confidence: 99%

“…(2) Biological evidence: it includes items from a biological origin, usually from the victim or the perpetrator (e.g., semen, vaginal fluid, oral fluid, sweat, blood, and other body fluids, hair, cells of the alleged perpetrator under a victim's finger nails, or epithelial cells of the alleged victim present on the penis of the perpetrator) [ 19 , 23 ], and botanical elements (e.g., pollen, plants, and wood). It is considered the most important type of evidence (especially semen) since it is very useful to prove that physical/sexual contact occurred and to identify a perpetrator through DNA studies [ 5 , 9 – 11 , 24 ].…”

Section: Forensic Evidencementioning

confidence: 99%

Biological Evidence Management for DNA Analysis in Cases of Sexual Assault

Magalhães

Dinis-Oliveira

Silva

et al. 2015

The Scientific World Journal

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Biological evidence with forensic interest may be found in several cases of assault, being particularly relevant if sexually related. Sexual assault cases are characterized by low rates of disclosure, reporting, prosecution, and conviction. Biological evidence is sometimes the only way to prove the occurrence of sexual contact and to identify the perpetrator. The major focus of this review is to propose practical approaches and guidelines to help health, forensic, and law enforcement professionals to deal with biological evidence for DNA analysis. Attention should be devoted to avoiding contamination, degradation, and loss of biological evidence, as well as respecting specific measures to properly handle evidence (i.e., selection, collection, packing, sealing, labeling, storage, preservation, transport, and guarantee of the chain custody). Biological evidence must be carefully managed since the relevance of any finding in Forensic Genetics is determined, in the first instance, by the integrity and quantity of the samples submitted for analysis.

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DNA recovery from different evidences in 300 cases of sexual assault

Cited by 10 publications

References 1 publication

Medical laboratory sciences and lectures a veritable tool in forensic science: A comparative review study of rape in Nigeria.

Medical laboratory sciences and lectures a veritable tool in forensic science: A comparative review study of rape in Nigeria.

Application and utility of alternative methods in isolation of pure cells from forensic biological mixtures in modern-day: a review

Biological Evidence Management for DNA Analysis in Cases of Sexual Assault

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