DNA Relatedness among Species of Sterigmatomyces and Fellomyces

Kurtzman, Cletus P.

doi:10.1099/00207713-40-1-56

Cited by 15 publications

(8 citation statements)

References 25 publications

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“…Although ballistoconidia are not present in Sterigmatomyces aphidis, there can be no doubt that these two species are closely related. On the basis of DNA relatedness data Kurtzman (20) postulated that these organisms are conspecific. Our RAPD fingerprinting results support the view that these fungi are genetically distinct (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…1 through 4 102T, were assessed for species delimitation by performing a RAPD PCR analysis. Previously, this method has been useful for identifying andl differentiating microorganisms (34,36), and we were interested in comparing the resolving power of RAPD analysis with the resolving power of nDNA reassociation experiments (20) and with the resolving power of enzyme and protein electrophoresis (39, 52). The PCR fragment patterns generated by four different arbitrary primers were studied.…”

Section: Resultsmentioning

confidence: 99%

“…Phenotypically, these three taxa are not like members of the genera Sterigmatomyces and Fellomyces. DNA comparisons (20) revealed that these three taxa are conspecific, although the reduced level of complementarity exhibited by Sterigmatomyces aphidis suggested that this organism is near the limit of relatedness exhibited by members of a species (21).…”

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confidence: 99%

“…Sterigmatomyces species that have conidia which separate at the distal ends of sterigmata and contain ubiquinone Q-10 have been transferred to the genera Fellomyces (xylose positive) and Kurtzmanomyces (xylose negative) (47,49). Sterigmatosporidium polymorphum ( 17) is the only teleomorphic species that corresponds to an anamorphic Fellomyces (20) counterpart.…”

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Molecular Characterization and Application of Random Amplified Polymorphic DNA Analysis of Mrakia and Sterigmatomyces Species

Messner¹,

Prillinger²,

Altmann³

et al. 1994

International Journal of Systematic Bacteriology

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The qualitative and quantitative monosaccharide spectra of purified yeast cell walls revealed that there are three phylogenetically distinct lineages of sterigma-forming basidiomycetous yeasts: (i) Kurtzmanomyces and Sterigmatomyces species, which contain high levels of mannose; (ii) Tilletiopsis species, which contain glucose, galactose, and small amounts of mannose; and (iii) Fellomyces, Kockovaella, Sterigmatosporidium, and Tsuchiyaea species, which appear to be closely related on the basis of their high levels of glucose and the presence of xylose. The yeast cell wall neutral sugars of Sporobolomyces antarcticus and Sterigmatomyces aphidis were similar to those of members of the genus Tilletiopsis. However, the possibility that these taxa are conspecific was eliminated by the results of a random amplified polymorphic DNA (RAPD) analysis. The conspecificity of Mrakia jiigida and Mrakia nivalis, the conspecificity of Mrakia gelida and Mrakia stokesii, and the conspecificity of Sterigmatomyces halophilus and Sterigmatomyces indicus were confirmed by RAPD analysis results. RAPD analysis was found to be a simple and highly sensitive method which can be used to differentiate species at the DNA level; it can replace nuclear DNA-nuclear DNA hybridization experiments for species identification, characterization, and delimitation.The genus Mrakia was proposed for Leucosporidium fngidum as a new genus of the Filobasidiaceae by Yamada and Komagata (51). The following four species that formerly had been placed in the genus Leucosporidium were transferred to the genus Mrakia: Mrakia Jizgida, Mrakia gelida, Mrakia nivalis, and Mrakia stokesii (7, 8, 51). A homothallic, apomictic life cycle, self-sporulating simple holobasidia bearing terminal, sessile basidiospores, fermentation of sugars, the presence of ubiquinone Q-8, production of extracellular amyloid compounds, and yeast cell walls containing xylose are the most distinctive features of Mrakia species (14,26,32,51). The fact that the genus Mrakia should be separated from phragmobasidial, smut-like Leucosporidium species (31) has been corroborated by the results of determinations of parts of the 18s and 26s rRNA sequences (6, 9,48) and ultrastructural septa1 pore data (37).The Mrakia species originally defined on the basis of their ability to ferment glucose and their ability to utilize nitrate or various carbon compounds (cellobiose, maltose, maltose, melibiose, raffinose) for growth (7, 8) turned out to be questionable taxa after investigations of the electrophoretic enzyme patterns (39, 52) and parts of the 18s and 26s rRNA sequences (6, 9, 48). The electrophoretic enzyme patterns (52) suggested that M. nivalis and M. stokesii are synonyms of M. fngida and M. gelida, respectively.The genus Sterigmatomyces was introduced by Fell (5) for nonfilamentous, yeast-like fungi that are characterized by a unique method of cell division. The yeast cells produce one or more sterigmata, each of which gives rise to a single conidium.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Molecular Characterization and Application of Random Amplified Polymorphic DNA Analysis of Mrakia and Sterigmatomyces Species

Messner¹,

Prillinger²,

Altmann³

et al. 1994

International Journal of Systematic Bacteriology

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“…Nuclear DNA reassociation is widely considered to be the test of choice for unequivocal assignment of unknown strains to known species (Bak & Stenderup, 1969 ;Dutta et al, 1967 ;Gue! ho et al, 1985 ;Kurtzman, 1990aKurtzman, , b, 1986Meyer, 1970 ;Meyer & Phaff, 1972 ;Ouchi et al, 1970 ;Springer & Krajewski, 1989 ;Vaughan-Martini, 1991). This approach calls for a comparison between the nDNAs from both the unknown culture and the type strain of the species previously indicated by conventional classification.…”

Section: Introductionmentioning

confidence: 99%

Non-radioactive dot-blot DNA reassociation for unequivocal yeast identification.

Cardinali

Liti

Martini

2000

International Journal of Systematic and Evolutionary Microbiology

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Unequivocal and rapid classification of yeast cultures cannot be accomplished exclusively on the basis of unstable phenotypic traits, but requires molecular tests relating to the whole genome (or the largest possible portion of it). DNA-DNA reassociation meets this requirement, although many procedures proposed for calculating overall sequence similarity are expensive and timeconsuming, thus restricting the possibility of unequivocal classification to a few specialized laboratories. A novel method, based on non-radioactive dotblot hybridization of whole genomic DNA, has shown high and reproducible proportionality between the detected signal and the amount of doublestranded DNA effectively present on the membrane. This procedure has been optimized to obtain, within two working days, DNA relatedness values between unknown cultures and the type strains of the species previously indicated by a few conventional tests. The effective ability of the method to discriminate strains belonging to different species has been tested within taxonomic models consisting of yeast type cultures already certified by spectrophotometric reassociation.

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