DNA repair and specific-locus mutagenesis in Neurospora crassa

Inoue, Hirokazu

doi:10.1016/s1383-5742(99)00079-4

Cited by 15 publications

(12 citation statements)

References 51 publications

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“…Isolation and characterization of strains sensitive to mutagens has led to an understanding of the range of DNA repair mechanisms. These include excision repair, recombination repair, photoreactivation repair, postreplication repair, and mismatch repair, all of which exist in Neurospora (287,364,692). Another mechanism, known as checkpoint control, affects the efficiency of DNA repair.…”

Section: Dna Repairmentioning

confidence: 99%

Lessons from the Genome Sequence ofNeurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism

Borkovich

Alex

Yarden

et al. 2004

Microbiol Mol Biol Rev

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We present an analysis of over 1,100 of the ∼10,000 predicted proteins encoded by the genome sequence of the filamentous fungus Neurospora crassa. Seven major areas of Neurospora genomics and biology are covered. First, the basic features of the genome, including the automated assembly, gene calls, and global gene analyses are summarized. The second section covers components of the centromere and kinetochore complexes, chromatin assembly and modification, and transcription and translation initiation factors. The third area discusses genome defense mechanisms, including repeat induced point mutation, quelling and meiotic silencing, and DNA repair and recombination. In the fourth section, topics relevant to metabolism and transport include extracellular digestion; membrane transporters; aspects of carbon, sulfur, nitrogen, and lipid metabolism; the mitochondrion and energy metabolism; the proteasome; and protein glycosylation, secretion, and endocytosis. Environmental sensing is the focus of the fifth section with a treatment of two-component systems; GTP-binding proteins; mitogen-activated protein, p21-activated, and germinal center kinases; calcium signaling; protein phosphatases; photobiology; circadian rhythms; and heat shock and stress responses. The sixth area of analysis is growth and development; it encompasses cell wall synthesis, proteins important for hyphal polarity, cytoskeletal components, the cyclin/cyclin-dependent kinase machinery, macroconidiation, meiosis, and the sexual cycle. The seventh section covers topics relevant to animal and plant pathogenesis and human disease. The results demonstrate that a large proportion of Neurospora genes do not have homologues in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. The group of unshared genes includes potential new targets for antifungals as well as loci implicated in human and plant physiology and disease

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Section: Dna Repairmentioning

confidence: 99%

Lessons from the Genome Sequence ofNeurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism

Borkovich

Alex

Yarden

et al. 2004

Microbiol Mol Biol Rev

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598

618

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“…This gene, mus-38, was characterized as a homolog of S. cerevisiae RAD1 (Hatakeyama et al, 1998;Inoue, 1999). Interestingly, N. crassa has another excision repair mechanism for the removal of UVinduced damage that is mediated by the MUS-18 protein (Ishii et al, 1991;Yajima et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis of the Neurospora crassa RAD14 homolog mus-43 and the RAD10 homolog mus-44 reveals that they belong to the mus-38 pathway of two nucleotide excision repair systems

et al. 2008

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Genetic analysis of the Neurospora crassa RAD14 homolog mus-43 and the RAD10 homolog mus-44 reveals that they belong to the mus-38 pathway of two nucleotide excision repair systemsMasahito Sato, Takaharu Niki, Takeru Tokou, Kouhei Suzuki, Makoto Fujimura and Akihiko Ichiishi * Sciences, Toyo University, Itakura, Oura-gun, Gunma 374-0193, Japan (Received 30 October 2007, accepted 19 November 2007 Saccharomyces cerevisiae Rad14 and Rad10 proteins are essential for nucleotide excision repair (NER). Rad14 is a UV-damaged DNA binding protein and Rad10 is a structure-specific endonuclease that functions in a complex with Rad1. In this study, we identified and characterized the RAD14 and RAD10 homolog genes in Neurospora crassa, which we named mus-43 and mus-44, respectively. Disruption of mus-43 and mus-44 conferred sensitivity to UV and 4-nitroquinoline 1-oxide, but not to methyl methanesulfonate, N-methyl-N'-nitro-N-nitrosoguanidine, camptothecin, hydroxyurea, or bleomycin. The mus-44 mutant was more sensitive to UV than the mus-43 mutant. Genetic analysis indicated that mus-43 and mus-44 are epistatic to mus-38 which is a homolog of the S. cerevisiae RAD1, but not to mus-18 which belongs to a second excision repair pathway. Immunological assays demonstrated that both mus-43 and mus-44 retained the ability to excise UV-induced cyclobutane pyrimidine dimers and 6-4 photoproducts, but that excision ability was completely abolished in the mus-43 mus-18 and mus-44 mus-18 double mutants. These double mutants exhibited extremely high sensitivity to UV. In mus-43 and mus-44 mutants, the UV-induced mutation frequency increased compared to that of the wild-type. The mus-44 mutants also exhibited a partial photoreactivation defect phenotype similar to mus-38. These results suggest that both mus-43 and mus-44 function in the mus-38 NER pathway, but not in the mus-18 excision repair pathway. Faculty of Life

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“…This gene, uvs-2, encodes a protein that shares 25.5% amino acid identity with Rad18 and interacts with a Rad6 homologue, MUS8 (7,8). In contrast to the yeast rad18 mutant, the uvs-2 mutant shows high mutation frequencies under both spontaneous and induced conditions (9)(10)(11). Although Rad18 is crucial for maintaining genomic integrity, Rad18 homologous genes in higher eukaryotes, including human beings, have not been reported yet.…”

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confidence: 99%

Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens

Tateishi

Sakuraba

Masuyama

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

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Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. The yeast Saccharomyces cerevisiae Rad18 protein plays a pivotal role in the process together with the Rad6 protein. Here, we have cloned a human homologue of RAD18, hRAD18. It maps on chromosome 3p24 -25, where deletions are often found in lung, breast, ovary, and testis cancers. In vivo, hRad18 protein binds to hHR6 protein through a conserved ring-finger motif. Stable transformants with hRad18 mutated in this motif become sensitive to UV, methyl methanesulfonate, and mitomycin C, and are defective in the replication of UV-damaged DNA. Thus, hRAD18 is a functional homologue of RAD18.

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DNA repair and specific-locus mutagenesis in Neurospora crassa

Cited by 15 publications

References 51 publications

Lessons from the Genome Sequence ofNeurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism

Lessons from the Genome Sequence ofNeurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism

Genetic analysis of the Neurospora crassa RAD14 homolog mus-43 and the RAD10 homolog mus-44 reveals that they belong to the mus-38 pathway of two nucleotide excision repair systems

Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens

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