DNA Repair of U.V.-damage in Heterochromatin and Euchromatin of Rat Liver

Tomura, Takanori; Lancker, Julien L. Van

doi:10.1080/09553008014551151

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…The induced photodamage are cyclobutane pyrimidine dimers (CPDs) and 6,4-photoproducts (6,4 PP; [4]). Both types of photolesions are formed between di-pyrimidines (TT, CC, TC and CT; [3]) and are localized in euchromatin and heterochromatin [12].…”

Section: Introductionmentioning

confidence: 99%

Photodamage repair pathways contribute to the accurate maintenance of the DNA methylome landscape upon UV exposure

et al. 2019

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Plants are exposed to the damaging effect of sunlight that induces DNA photolesions. In order to maintain genome integrity, specific DNA repair pathways are mobilized. Upon removal of UV-induced DNA lesions, the accurate re-establishment of epigenome landscape is expected to be a prominent step of these DNA repair pathways. However, it remains poorly documented whether DNA methylation is accurately maintained at photodamaged sites and how photodamage repair pathways contribute to the maintenance of genome/methylome integrities. Using genome wide approaches, we report that UV-C irradiation leads to CHH DNA methylation changes. We identified that the specific DNA repair pathways involved in the repair of UV-induced DNA lesions, Direct Repair (DR), Global Genome Repair (GGR) and small RNA-mediated GGR prevent the excessive alterations of DNA methylation landscape. Moreover, we identified that UV-C irradiation induced chromocenter reorganization and that photodamage repair factors control this dynamics. The methylome changes rely on misregulation of maintenance, de novo and active DNA demethylation pathways highlighting that molecular processes related to genome and methylome integrities are closely interconnected. Importantly, we identified that photolesions are sources of DNA methylation changes in repressive chromatin. This study unveils that DNA repair factors, together with small RNA, act to accurately maintain both genome and methylome integrities at photodamaged silent genomic regions, strengthening the idea that plants have evolved sophisticated interplays between DNA methylation dynamics and DNA repair.

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Section: Introductionmentioning

confidence: 99%

Photodamage repair pathways contribute to the accurate maintenance of the DNA methylome landscape upon UV exposure

et al. 2019

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“…The induced photodamage are cyclobutane pyrimidine dimers (CPDs) and 6,4-photoproducts (6,4 PP, Britt, 1995). Both types of photolesions are formed between di-pyrimidines (TT, CC, TC, and CT; Molinier, 2017) and are localized in euchromatin and heterochromatin (Tomura and van Lancker, 1980). In plants, UV-induced DNA lesions are preferentially repaired by Direct Repair (DR) that is a light-dependent error-free process catalyzed by enzymes called DNA photolyases (Sancar, 2004).…”

Section: Introductionmentioning

confidence: 99%

Photodamage repair pathways contribute to the accurate maintenance of the DNA methylome landscape upon UV exposure

Graindorge

Cognat

Berens

et al. 2019

Preprint

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Plants are exposed to the damaging effect of sunlight that induces DNA photolesions. In order to maintain genome integrity, specific DNA repair pathways are mobilized. Upon removal of UV-induced DNA lesions, the accurate re-establishment of epigenome landscape is expected to be a prominent step of these DNA repair pathways. However, it remains poorly documented whether DNA methylation is accurately maintained at photodamaged sites and how photodamage repair pathways contribute to the maintenance of genome/methylome integrities.Using genome wide approaches, we report that UV-C irradiation leads to asymmetric DNA methylation changes. We identified that the specific DNA repair pathways involved in the repair of UV-induced DNA lesions, Direct Repair (DR) and Global Genome Repair (GGR), prevent the excessive alterations of DNA methylation landscape. Moreover, we identified that UV-C irradiation induced chromocenter reorganization and that photodamage repair factors control this dynamics. The methylome changes rely on misregulation of maintenance, de novo and active DNA demethylation pathways highlighting that molecular processes related to genome and methylome integrities are closely interconnected. Importantly, we identified that photolesions are sources of DNA methylation changes in both, constitutive and facultative heterochromatin. This study unveils that DNA repair factors, together with small RNA, act to accurately maintain both genome and methylome integrities at photodamaged silent genomic regions, strengthening the idea that plants have evolved sophisticated interplays between DNA methylation dynamics and DNA repair. Availability of data and materialsWGBS, DNA-seq and small RNA-seq raw data generated in this work are publicly accessible through the GEO registration number : GSE132750. Authors' contributionsSG and VC performed bioinformatics. PJtoB performed cytogenetics, developed the automated image analysis program with JMu and analyzed the data. SG, VC, PJtoB and JMu wrote the methods section. JM designed experiments, prepared the biological samples, analyzed the data and wrote the manuscript. All authors read and approved the final manuscript.

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Inter- and intra-chromosomal distribution of chromatid breaks induced by X-rays during G2 in human lymphocytes

Pelliccia¹,

Micheli²,

Olivieri³

1985

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

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DNA Repair of U.V.-damage in Heterochromatin and Euchromatin of Rat Liver

Cited by 4 publications

References 17 publications

Photodamage repair pathways contribute to the accurate maintenance of the DNA methylome landscape upon UV exposure

Photodamage repair pathways contribute to the accurate maintenance of the DNA methylome landscape upon UV exposure

Photodamage repair pathways contribute to the accurate maintenance of the DNA methylome landscape upon UV exposure

Inter- and intra-chromosomal distribution of chromatid breaks induced by X-rays during G2 in human lymphocytes

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