DNA Replication Pattern of the Y Chromosome in XYY and XXYY Males

Borgaonkar, D. S.; Herr, Helen M.; Nissim, J.

doi:10.1093/oxfordjournals.jhered.a108028

Cited by 4 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, direct comparison and conclusions about the labelling pattern in different patients based on these investigations do not seem justified. Two recent publications (Nielsen et al 1970, Borgaonkar et al 1970) report synchronous as well as asynchronous labelling of the Y chromosomes in the same patient. This type of labelling pattern is found also in our two patients but only during the terminal stage of DNA synthesis.…”

Section: Discussionmentioning

confidence: 89%

“…The results of previous autoradiographic studies of the YY labelling pattern are conflicting, some patients being reported to have synchronously labelled Y's (Balodimos et al 1966, Nielsen et al 1966, Franks et al 1967, Boczkowski & Casey 1967, others to have asynchronously labelled Y's (Kikuchi & Sandberg 1965, Kosenow & Pfeiffer 1966, Kelly et al 1957. In two recent papers (Nielsen et al, 1970, Borgaonkar et al 1970, the pat-tern is described as synchronous as well as asynchronous in the same individual.…”

mentioning

confidence: 99%

See 1 more Smart Citation

DNA synthesis of human XYY cells

Santesson¹,

Böök²,

Gustavson³

1972

Clinical Genetics

View full text Add to dashboard Cite

The autoradiographic YY labelling pattern of the DNA replication in 47, XYY cells of two patients has been studied. Both Y chromosomes began DNA replication simultaneously but later than the rest of the chromosomes. During later stages the YY labelling pattern in both patients was correlated to the position of the cells in S, as indicated by the cell grain count: in cells with more than approximately 500 grains the synchronous labelling of the Y chromosomes was the rule; whereas during later stages in cells with approximately 100–400 grains, synchronous as well as asynchronous labelling in the same patient was found. In cells with less than about 100 grains, both Y chromosomes usually had completed replication. No obvious difference between the labelling pattern of the two Y chromosomes was found when cells from the 2 patients were compared at the same stage of S. Our results demonstrate the importance of analysing cells of comparable developmental stages of S when comparing DNA labelling patterns of different XYY individuals in search of a possible correlation between labelling pattern and phenotypic expression of the syndrome. In one of the patients the QM fluorescence pattern as well as the autoradiographic pattern of the same cells was studied. The characteristic intense fluorescence pattern of both Y chromosomes was found even in cells where the autoradiographic labelling pattern indicated distinct asynchrony between the two Y chromosomes. The significance of these findings is discussed.

show abstract

Section: Discussionmentioning

confidence: 89%

mentioning

confidence: 99%

DNA synthesis of human XYY cells

Santesson¹,

Böök²,

Gustavson³

1972

Clinical Genetics

View full text Add to dashboard Cite

show abstract

“…Until recently, however, anomalies usually have been identified through reliance upon conventional light microscopy and on the ability of the investigator to identify the Y by morphologic criteria. Autoradiographic studies, although of questionable value (Borgaonkar et al 1970a) in the identification of the human Y chromosome, have been used sometimes to confirm the presence of the extra Y chromosome. Errors in the identification of an extra Y chromosome have been made in the past and are likely to continue.…”

mentioning

confidence: 99%

Accurate identification of the human Y chromosome

1974

View full text Add to dashboard Cite

Examination by light microscopy o f cells of various constitutions with one Y, two Ys, or no Y was made by six experienced cytogeneticists in order to ascertain accuracy in identification of the Y chromosome by morphologic criteria. Results showed that this approach is subject to error, especially if the quality of the spreads is not very good. Refined techniques and/or additional information appear to be essential.

show abstract