DNA-RFLP analysis and genotyping of HLA-DR and DQ antigens

Bidwell, J. L.

doi:10.1016/0167-5699(88)91351-5

Cited by 189 publications

(105 citation statements)

References 41 publications

Supporting

Mentioning

102

Contrasting

Unclassified

Order By: Relevance

“…The DR type was identified by DR allele-specific patterns for HLA-DR4 and DR1 (14.8/6.1/5.4/2.7 kb for DR4 and 6 3 4 . 4 kb for DR1) (30).…”

Section: Methodsmentioning

confidence: 99%

Immunoglobulin kappa genotype confers risk of rheumatoid arthritis among hla–dr4 negative individuals

Moxley

1989

Arthritis & Rheumatism

View full text Add to dashboard Cite

Because DNA polymorphisms of immunoglobulin kappa confer risk for rheumatoid arthritis (RA) and not all persons with RA have the HLA-DR4 marker, genomic polymorphisms of immunoglobulin kappa and HLA-DRP were determined in white patients with RA. Compared with control subjects matched for DRP genotype, the homozygous genotype of the constant segment of immunoglobulin kappa (C,) was more frequent in the subgroups of RA patients without the DRP genotype corresponding to HLA-DR4 (relative risk 6.2, P < 0.01) and patients without DR4 or DR1 (relative risk 6.7, P = 0.013), but not in the DR4+ RA subgroup. Therefore, RA may be a genetically heterogeneous disease, with HLA-DR4 marking one genetic subset and the homozygous C, genotype marking another.

show abstract

“…The DR type was identified by DR allele-specific patterns for HLA-DR4 and DR1 (14.8/6.1/5.4/2.7 kb for DR4 and 6 3 4 . 4 kb for DR1) (30).…”

Section: Methodsmentioning

confidence: 99%

Immunoglobulin kappa genotype confers risk of rheumatoid arthritis among hla–dr4 negative individuals

Moxley

1989

Arthritis & Rheumatism

View full text Add to dashboard Cite

show abstract

“…HLA-DRBI and HLA-DQAl typing was performed by restriction fragment length polymorphism (RFLP) analysis using Taq I digested genomic DNA and complementary DNA (cDNA) probes for Ihc DRBI and DQAI genes as previously described [24], HLA-DRBI and HLADQAl specificities are given in accordance with specific RFLP bands assigned al the 10th International Histoeompatibility Testing Workshop [25]. In addition.…”

Section: Hla-drbi Hla-dqat and Hla-dqbi Typingmentioning

confidence: 99%

Anti-centromere antibodies (ACA) in systemic sclerosis patients and their relatives: a serological and HLA study

McHugh

Whyte

Artlett

et al. 1994

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYAutoantibody reaclivily to cenlromere prolcins CENP-A. CENP-B and CENP-C was examined in 58 patienls with systemic sclerosis (SSc). 218 first degree relatives and 22 spouses, HLA class II typing for HLA-DRBI and HLA-DQAI was performed by reslriction fragment lenglh polymorphism (RFLP) analysis in 50 families, and HLA-DRBI, HLA-DQAI and HLA-DQBl typing was performed by olignueleolitlc typing in 44 families. Eleven probands and two relatives had ACA. The two relatives with ACA also had SSc. One relative was an identical twin sister of a proband with ACA and Iheother relative was a sister of a proband with ACA. All ACA-positivc probands and relatives were female, and all recognized CENP-A. CENP-B and CENP-C. The presence of at least one HLADQBl allele not coding for leueine at position 26 of the first domain appeared nceessary. although not sufficient for the generation of ACA, Therefore within SSc families ACA is strongly associated with female gender and disease phenolype, and is at least in part genetically determined.

show abstract

“…This was performed using the x^ statistic with Yates' correction, RESULTS DR antigen frequency DR polymorphism was analysed by RFLP of HLA-DRB and DQA genes in the patient and control groups, using the characteristic RFLP fragments generated by each probe to define the HLA-DR genotype for each individual (Bidwell, 1988). The results are summarized in Table 1.…”

Section: Statisticat Anatysismentioning

confidence: 99%

Common variable immunodeficiency is associated with polymorphic markers in the human major histocompatibility complex

Howe

Farrant

et al. 1991

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYCommon variable immunodeficiency (CVI) is a heterogeneous condition characterized by arrest in B cell differentiation, A high frequency of null alleles ofthe C4 gene has been reported in patients with this disorder. We investigated the restriction fragment length polymorphisms (RFLP) ofthe MHC class II genes HLA-DRB, DQA, and DQB, the class III gene C4 and the tumour necrosis factoralpha (TNF-a) gene in 40 Caucasian patients. The results showed an increase in HLA-DR3 in patients (40% vs 30-5%), but, more significantly, there was a striking increase in the number of CVI patients who carried a deletion ofthe C4A gene (46% vs 25%), In both patients and controls there was strong allelic association between HLA-DR3 and C4A deletion, and HLA-DR3 and TNF-a, Our results suggest that genes present on an extended haplotype containing these three polymorphisms contribute to genetic susceptibility to CVI,

show abstract

DNA-RFLP analysis and genotyping of HLA-DR and DQ antigens

Cited by 189 publications

References 41 publications

Immunoglobulin kappa genotype confers risk of rheumatoid arthritis among hla–dr4 negative individuals

Immunoglobulin kappa genotype confers risk of rheumatoid arthritis among hla–dr4 negative individuals

Anti-centromere antibodies (ACA) in systemic sclerosis patients and their relatives: a serological and HLA study

Common variable immunodeficiency is associated with polymorphic markers in the human major histocompatibility complex

Contact Info

Product

Resources

About