DNA sequence analysis of the composite plasmid pTC conferring virulence and antimicrobial resistance for porcine enterotoxigenic Escherichia coli

Fekete, Péter Zs.; Brzuszkiewicz, Elżbieta; Blum-Oehler, Gabriele; Olasz, Ferenc; Szabó, Márton; Gottschalk, Gerhard; Hacker, Jörg; Nagy, Béla

doi:10.1016/j.ijmm.2011.07.003

Cited by 17 publications

(27 citation statements)

References 20 publications

(24 reference statements)

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“…The toxin-specific locus caused the enterotoxigenicity of the strain, which contains two heat-stable enterotoxin genes, sta and stb. In this example, the authors observed coselection of virulence and tetracycline resistance, but they did not analyze the biological cost of carrying this virulence plasmid (234). Coselection of tetracycline was also observed in C. perfringens in a study that analyzed the nonreplicating transposon Tn916, which is involved in the conjugation of a replicating plasmid carrying putative virulence genes and two tetracycline resistance mechanisms, tetA(P) (efflux system) and tetB(P), which provide ribosomal protection (235,236).…”

Section: Plasmidsmentioning

confidence: 99%

Antimicrobial Resistance and Virulence: a Successful or Deleterious Association in the Bacterial World?

Beceiro

Tomás²,

Bou³

2013

Clin Microbiol Rev

855

630

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SUMMARY Hosts and bacteria have coevolved over millions of years, during which pathogenic bacteria have modified their virulence mechanisms to adapt to host defense systems. Although the spread of pathogens has been hindered by the discovery and widespread use of antimicrobial agents, antimicrobial resistance has increased globally. The emergence of resistant bacteria has accelerated in recent years, mainly as a result of increased selective pressure. However, although antimicrobial resistance and bacterial virulence have developed on different timescales, they share some common characteristics. This review considers how bacterial virulence and fitness are affected by antibiotic resistance and also how the relationship between virulence and resistance is affected by different genetic mechanisms (e.g., coselection and compensatory mutations) and by the most prevalent global responses. The interplay between these factors and the associated biological costs depend on four main factors: the bacterial species involved, virulence and resistance mechanisms, the ecological niche, and the host. The development of new strategies involving new antimicrobials or nonantimicrobial compounds and of novel diagnostic methods that focus on high-risk clones and rapid tests to detect virulence markers may help to resolve the increasing problem of the association between virulence and resistance, which is becoming more beneficial for pathogenic bacteria.

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Section: Plasmidsmentioning

confidence: 99%

Antimicrobial Resistance and Virulence: a Successful or Deleterious Association in the Bacterial World?

Beceiro

Tomás²,

Bou³

2013

Clin Microbiol Rev

855

630

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“…Conjugation frequency was calculated as a ratio between the number (CFU/ml) of transconjugants and that of the recipient strain J5-3. ETEC strain 2173 served as a reference for the transferability of the tet(B)-mediating plasmid pTC (Fekete et al, 2012).…”

Section: Identification and Characterisation Of Etec Plasmids Mediatimentioning

confidence: 99%

“…Parental and transconjugant strains were tested by PCR for the presence of antimicrobial resistance genes related to mobile genetic elements and typical ETEC virulence factors, including the flanking regions of the stb gene specific to the pTC plasmid of F18 + ETEC 2173 (Fekete et al, 2012). Genes intI1, qacEΔ1 and sul1 as parts of the 3' conserved region of the class 1 integron were also tested by PCR.…”

Section: Detection Of Antimicrobial Resistance and Virulence Genesmentioning

confidence: 99%

“…Genes encoding the above virulence factors are located on ETEC virulence plasmids (Gyles, 1994;Fekete et al, 2012); however, several other mobile virulence elements such as pathogenicity islands (PAIs), bacteriophages and transposons may also contribute to the transfer of virulence genes between E. coli strains (Hacker et al, 1997). As an example, heat-stable enterotoxin genes sta and stb are part of transposons: sta is carried by Tn1681 (So and McCarthy, 1980), and stb was described as a part of Tn4521 (Hu and Lee, 1988).…”

mentioning

confidence: 99%

“…In our previous studies on the representative porcine post-weaning F18 + ETEC strain 2173 we described and sequenced the large conjugative plasmid pTC (sta + , stb + , tetB + ) as being responsible for the enterotoxigenicity and tetracycline resistance [tet(B)] of the host strain (Nagy et al, 1990;Fekete et al, 2012). Among Enterobacteriaceae, tetracycline resistance is encoded mainly by tetracycline efflux proteins that remove tetracycline from the bacterial cytoplasm by a process most often encoded by tet(A), tet (B), tet(C), tet(D), tet(E), and tet(G) genes.…”

mentioning

confidence: 99%

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Conjugative IncF and IncI1 plasmids with tet(A) and class 1 integron conferring multidrug resistance in F18+ porcine enterotoxigenic E. coli

Szmolka

Lestár

Pászti

et al. 2015

Acta Veterinaria Hungarica

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Enterotoxigenic E. coli (ETEC) bacteria frequently cause watery diarrhoea in newborn and weaned pigs. Plasmids carrying genes of different enterotoxins and fimbrial adhesins, as well as plasmids conferring antimicrobial resistance are of prime importance in the epidemiology and pathogenesis of ETEC. Recent studies have revealed the significance of the porcine ETEC plasmid pTC, carrying tetracycline resistance gene tet(B) with enterotoxin genes. In contrast, the role of tet(A) plasmids in transferring resistance of porcine ETEC is less understood. The objective of the present study was to provide a comparative analysis of antimicrobial resistance and virulence gene profiles of porcine post-weaning ETEC strains representing pork-producing areas in Central Europe and in the USA, with special attention to plasmids carrying the tet(A) gene. Antimicrobial resistance phenotypes and genotypes of 87 porcine ETEC strains isolated from cases of postweaning diarrhoea in Austria, the Czech Republic, Hungary and the Midwest USA was determined by disk diffusion and by PCR. Central European strains carrying tet(A) or tet(B) were further subjected to molecular characterisation of their tet plasmids. Results indicated that > 90% of the ETEC strains shared a common multidrug resistant (MDR) pattern of sulphamethoxazole (91%), tetracycline (84%) and streptomycin (80%) resistance. Tetracycline resistance was most frequently determined by the tet(B) gene (38%), while tet(A) was identified in 26% of all isolates with wide ranges for both tet gene types between some countries and with class 1 integrons and resistance genes co-transferred by conjugation. The virulence gene profiles included enterotoxin genes (lt, sta and/or stb), as well as adhesin genes (k88/f4, f18). Characterisation of two representative tet(A) plasmids of porcine F18 + ETEC from Central Europe revealed that the IncF plasmid (pES11732) of the Czech strain (~120 kb) carried tet(A) in association with catA1 for chloramphenicol resistance. The IncI1 plasmid (pES2172) of the Hungarian # Present address: Ceva-Phylaxia, Szállás u.

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Multidrug resistance and multivirulence plasmids in enterotoxigenic and hybrid Shiga toxin-producing/enterotoxigenic Escherichia coli isolated from diarrheic pigs in Switzerland

Brilhante

Perreten

Donà

2019

The Veterinary Journal

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DNA sequence analysis of the composite plasmid pTC conferring virulence and antimicrobial resistance for porcine enterotoxigenic Escherichia coli

Cited by 17 publications

References 20 publications

Antimicrobial Resistance and Virulence: a Successful or Deleterious Association in the Bacterial World?

Antimicrobial Resistance and Virulence: a Successful or Deleterious Association in the Bacterial World?

Conjugative IncF and IncI1 plasmids with tet(A) and class 1 integron conferring multidrug resistance in F18+ porcine enterotoxigenic E. coli

Multidrug resistance and multivirulence plasmids in enterotoxigenic and hybrid Shiga toxin-producing/enterotoxigenic Escherichia coli isolated from diarrheic pigs in Switzerland

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