DNA Sequence of a Gene Encoding a BALB/c Mouse L ^d Transplantation Antigen

Abstract

The sequence of a gene, denoted 27.5, encoding a transplantation antigen for the BALB/c mouse has been determined. Gene transfer studies and comparison of the translated sequence with the partial amino acid sequence of the Ld transplantation antigen establish that gene 27.5 encodes an Ld polypeptide. A comparison of the gene 27.5 sequence with several complementary DNA sequences suggests that the BALB/c mouse may contain a number of closely related L-like genes. Gene 27.5 has eight exons that correlate with the structural domains of the transplantation antigen.

Mouse histocompatibility genes: structure and organisation of a Kd gene.

“…Gene 2.14 contains a nucleotide sequence that is highly homologous to two other H-2 genes (Steinmetz et al, 198 lb;Moore et al, 1982). The first exon of the gene encodes a polypeptide consisting of 21 amino acids.…”

“…Large fragments of DNA cloned into cosmids were employed to show that the genome of the BALB/c mouse has -35 class I H-2 genes (Steinmetz et al, 1982). Two of these genes, one coming from the Qa region and the other one coding for the Ld antigen, have been sequenced (Steinmetz et al, 1981b;Moore et al, 1982). The analysis of these two sequences revealed a high degree of homology indicating that Qa genes (and most likely also those of Tla) have a very similar structure to the classical H-2 genes.…”

Organ injury associated with extrathymic induction of immune tolerance in doubly transgenic mice.

“…The H-2Ld gene (LL) or a hybrid gene (EL), constructed by placing a 4.7-kilobase (kb) BamHI Ld-gene fragment downstream of a 0.2-kb rat pancreatic elastase I promoter fragment (14,15), was microinjected into F2 zygotes. To make doubly transgenic animals, EL transgenic mice of the H-2b haplotype were mated to 2C TCR transgenic mice of the same haplotype (12).…”

Identification of the Cloned Gene for the Murine Transplantation Antigen H-2Kbby Hybridization with Synthetic Oligonucleotides

“…As an alternative, we used synthetic oligonucleotide probes, constructed to display greater hybridization specificity than do cDNA probes, to distinguish the H-2Kb gene from the rest of the class I gene family. By comparing published class I sequences from our (9,11,(13)(14)(15) and other (2,4,8,16,19) laboratories, we have defined an H-2Kb-associated oligonucleotide sequence (19 bases) within the coding sequence for amino acid residues 94 through 100. A recombinant genomic DNA library was screened with class I probes, and 35 positive clones were subsequently tested with the specific nonadecanucleotide probe.…”

“…The second region represents the coding sequence for amino acid residues 94 through 100. Every murine class I antigen has a different sequence in this region (Table 1) [11,13,14], Db [15], Ld [2,8], 27.1 [19], Kd [4], Dd [9], and Kk [16]). Oligonucleotides were synthesized by the phosphotriester solid-phase method as described previously (Tan et al, in press).…”