DNA-stabilized silver nanoclusters with guanine-enhanced fluorescence as a novel indicator for enzymatic detection of cholesterol

Deng, Min; Peng, Yunlin; Zhang, Liangliang; Wang, Xiangyu; Ge, Jia; Jiang, Jian‐Hui; Yu, Ru‐Qin

doi:10.1039/c3ay40118a

Cited by 28 publications

(20 citation statements)

References 34 publications

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“…Jiang and Yu coupled the H2O2 quenching reaction to an enzymatic reaction using cholesterol as the substrate, where H2O2 was consumed. They were able to detect as little as 200 nM cholesterol [133]. Recently, Chu and co-workers detected highly oxidative species inside cells using AuNCs decorated on silica nanoparticles [134].…”

Section: Reactive Quenchersmentioning

confidence: 99%

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Liu

2014

TrAC Trends in Analytical Chemistry

198

141

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In the past decade, fluorescent silver, gold and copper nanoclusters (Ag, Au and CuNCs) have emerged as a new class of signaling moiety for biosensor development. Compared to semiconductor quantum dots, metal NCs have less toxicity concerns and can be more easily conjugated to biopolymers. Due to their extremely small size, these NCs need a stabilizing ligand. Many polymers, proteins and nucleic acids have been reported to stabilize NCs. In particular, many DNA sequences produce highly fluorescence NCs. Coupling these DNA stabilizers with other sequences such as aptamers has generated a large number of biosensors. In this review, the synthesis of DNA and nucleotide-templated NCs is first summarized; their chemical interactions are also discussed. In the second part, the properties of NCs such as fluorescence quantum yield, emission wavelength and lifetime, structure and photostability are briefly reviewed. In the last part, various sensor design strategies using these NCs are categorized into the following four classes: 1) fluorescence de-quenching; 2) generation of templating DNA sequences to produce NCs; 3) change of nearby environment; and 4) reacting with heavy metal ions or other quenchers. Finally, the future trends in this field are discussed.

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Section: Reactive Quenchersmentioning

confidence: 99%

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Liu

2014

TrAC Trends in Analytical Chemistry

198

141

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“…Among these various biological scaffolds are also nucleic acids, which have recently become the center of many new biostabilized NC studies [25,29,31,66]. Multiple metal NCs have shown to self-assemble on various DNA sequences, with their fundamental synthesis mechanisms and key applications coming to light.…”

Section: Dna-mediated Metal Nanoclustersmentioning

confidence: 99%

Biomediated Atomic Metal Nanoclusters: Synthesis and Theory

Griep¹,

West²,

Sellers³

et al. 2015

Handbook of Nanoparticles

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Fluorescent metal nanoclusters are an emerging class of multifunctional materials engineered at the singleatom level, with dimensions approaching the Fermi wavelength of electrons that offer competitive functionalities of traditional semiconductor QDs including tunable emission, ease of conjugation, extended photostability, and high quantum yield. With the additional advantages of being composed of nontoxic/ biocompatible materials, function with a fraction of the metal content, greatly reduced size for enhanced cellular uptake, opportunity for two-photon absorption at biologically relevant wavelengths, and demonstrated renal evacuation efficacy for in vivo applications, metal nanoclusters hold substantial promise. More recently the development of hybrid atomic cluster synthesis routes has expanded the materials' multifunctional capabilities. This chapter will summarize the current high-yield synthesis and functionalization strategies for producing monodisperse pure metal and hybrid nanocluster materials from both wet chemistry and newly developed biomediated nanocluster synthesis methodologies, involving protein and DNA hosts. The role of the bio-host and surface functional groups on the nanoclusters formation, stability, and physical properties will be detailed through recent experimental and theoretical simulation efforts.

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“…Various methods have been reported for the determination of cholesterol, including spectrophotometry (3), fluorescent spectrometry (4,5), chemiluminescence (6), high-performance liquid chromatography (7) and electrochemical methods (8)(9)(10)(11). Some of these have certain disadvantages, such as scarce reagents (3,5), expensive equipment (7), poor selectivity or sensitivity (8,9) or excessive operation times (10,11).…”

Section: Introductionmentioning

confidence: 99%

“…Some of these have certain disadvantages, such as scarce reagents (3,5), expensive equipment (7), poor selectivity or sensitivity (8,9) or excessive operation times (10,11). A few of the analytical methods reported in recent years have led to advancements in the determination of cholesterol in specific samples, however, special pretreatment or techniques are usually needed (12).…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive and selective electrochemiluminescence determination of cholesterol utilizing a functional electrode with a core–shell nanostructure

Cheng

2015

Luminescence

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A highly sensitive and selective method for the determination of cholesterol is required to evaluate trace amounts of cholesterol in test samples. In this work, selected gold nanoparticles (AuNPs) and 5-amino-2-mercapto-1,3,4-thiadiazole (AMT) were used and a thin film of three-dimensional gold-AMT core-shell nanoparticles (p-AMT-AuNPs) was prepared using an electrochemical method. Cholesterol oxidase was then bonded to the film surface to give a functional electrode. Based on catalysis by the electrode functionalized for cholesterol and a luminol-H2 O2 electrochemiluminescence (ECL) system, a highly sensitive and selective ECL method was developed for the determination of cholesterol. Under optimized conditions, ECL intensity showed a good linear relationship with cholesterol over the concentration range 0.05-11.0 µg/ml, with a correlation coefficient of 0.999 and a limit of detection of 0.02 µg/ml. The proposed method was used to determine cholesterol in dairy products with a relative standard deviation of < 1.8% and recovery rates of 98.1-104%.

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DNA-stabilized silver nanoclusters with guanine-enhanced fluorescence as a novel indicator for enzymatic detection of cholesterol

Cited by 28 publications

References 34 publications

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Biomediated Atomic Metal Nanoclusters: Synthesis and Theory

Highly sensitive and selective electrochemiluminescence determination of cholesterol utilizing a functional electrode with a core–shell nanostructure

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