Dna Strand Breakage in Freshwater Mussels (Anodonta Grandis) Exposed to Lead in the Laboratory and Field

Black, Marsha C.; Ferrell, Jennifer R.; Horning, Renée C.; Martin, Lene

doi:10.1897/1551-5028(1996)015<0802:dsbifm>2.3.co;2

Cited by 12 publications

(11 citation statements)

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“…Any changes to DNA may have long-lasting effects but the self-repairing capability of DNA may affect the precise interpretation of the relevant bioassays (Connell et al 1999). According to Black et al (1996), the presence of high molecular weight DNA may not necessarily be a result of the exposure to low impact areas but rather, due to the induction of DNA repair mechanisms after the exposure to a highly impacted area. In fact, many authors have demonstrated that toxicant burdens or exposure duration must reach a threshold level before such repair systems can be initiated (Pavlica et al 2001;Hoff et al 2003).…”

Section: Protein Fractions In Gills and Muscles Of Fishmentioning

confidence: 99%

Assessing metal, protein, and DNA profiles in Labeo rohita from the Indus River in Mianwali, Pakistan

Chaudhry

Jabeen

2010

Environ Monit Assess

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This study assessed metals in water and different tissues of Labeo rohita and the impact of these metals on DNA and proteins as biomarkers of gills and muscles of these fish from three different polluted sites (reference or low = KW, medium = CH and high = SK) of the Indus River, Pakistan. The Mn, Pb, Cu, Zn, Hg, and Cr levels in water, gills, liver, muscles, and skin of these fish were compared with the international permissible levels. All metals except Pb and Hg in water were within the acceptable limits of drinking water. In contrast, the Mn, Hg, and Cr levels in the fish tissues were higher than their permissible limits for fish as a human food. Here, the gills contained higher metals than the other tissues. Different patterns of biomarkers were found in fish from these sites. While the gills did not show four protein bands (55, 30, 18.4, and 16.4 kDa), the muscles showed four new protein bands (100, 85, 45, and 20 kDa) for fish from the medium and high polluted sites as compared to the reference or low polluted site. The fish from the CH and SK sites of the Indus River contained low molecular weight DNA in their gills but high molecular weight DNA in their muscles when compared with the KW site. This study suggests that the proteins and DNA profiles of L. rohita could be used as biomarkers to assess the impact of potential environmental stressors such as metals on the freshwater systems.

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Section: Protein Fractions In Gills and Muscles Of Fishmentioning

confidence: 99%

Assessing metal, protein, and DNA profiles in Labeo rohita from the Indus River in Mianwali, Pakistan

Chaudhry

Jabeen

2010

Environ Monit Assess

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“…Yang et al (2003) found a significant association between the occurrence of barbell abnormalities in brown bullheads collected from Lake Erie tributaries and NPH-like metabolites in their bile. Exposure to Pb has been shown to result in DNA damage in both marine and freshwater bivalves (Avery et al, 1996;Black et al, 1996). In vitro exposure of human hepatocytes to the parent PCB mixture Aroclor 1254 significantly increased DNA adduct formation (Borlak et al, 2003).…”

Section: Origin Of the Exposures And Nature Of The Genotoxinsmentioning

confidence: 99%

DNA Strand Length and EROD Activity in Relation to Two Screening Measures of Genotoxic Exposure in Great Lakes Herring Gulls

et al. 2005

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We collected tissues from herring gulls (Larus argentatus) nesting within and outside of the Great Lakes basin. Genotoxin exposure was assessed as fluorescent aromatic compounds (FACs) in bile and SOS Chromotest-inducing activity in muscle extracts. We determined whether these exposures were associated with decreased erythrocyte DNA strand length and/or induction of hepatic ethoxyresorufin-O-deethylase (EROD) activity. FACs were detected in all bile samples. Most muscle extracts produced a positive or marginal SOS response in the presence of S9. SOS induction potentials were strongly associated with dietary trophic level. The median molecular length of DNA isolated from erythrocytes for 14 of 17 adult and 10 of 11 prefledgling collections was reduced compared to the modal class for their respective age group suggesting widespread DNA damage. DNA damage was greatest in gulls from Saginaw Bay, Lake Huron. Median EROD activity in both adults and prefledglings from remote locations was significantly lower than that of gulls from the lower Great Lakes and was not associated with concentrations of benzo[a]pyrene (B[a]P)-like FACs. Our results indicate Great Lakes herring gulls were exposed to genotoxins and Ah-receptor activating agents in biologically significant concentrations in the early 1990s. These agents appear to be persistent bioaccumulative compounds and/or their metabolites.

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“…The DNA repair mechanism was also found in the gills of the 0.0050 mg L -1 Hg 2+ groups and in the hepatopancreas of the 0.0025 mg L -1 Hg 2+ groups. Black et al (1996) suggested that toxicant concentrations in the target tissues might need to reach a threshold level before the repair systems were initiated. Hg 2+ was demonstrated to be able to displace zinc ions from zinc finger structures (O'Connor et al, 1993;Asmuss et al, 2000), a frequent motif in DNA repair enzyme.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that DNA damage could be induced by excessive free radicals which are associated with free radical scavengers (Winston and Di Giulio, 1991). Recently, most researches on DNA damage induced by Cd and Pb have been carried out in fish Khuda-Bukhsh, 2001, 2004) and bivalves (Black et al, 1996;Pruski and Dixon, 2002), but barely in crustaceans, and few studies have focused on Hg 2+ .…”

Section: Introductionmentioning

confidence: 99%

Effects of mercuric chloride on antioxidant system and DNA integrity of the crab Charybdis japonica

Zhang

Pan

Miao

et al. 2009

J. Ocean Univ. China

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Mercury (Hg) is one of the commonly encountered heavy metals, which is widespread in inshore sediments of China. In order to investigate the toxicity of Hg on marine invertebrates, we studied the effects of the divalent mercuricion (Hg 2+ ) (at two final concentrations of 0.0025 and 0.0050 mg L -1 , prepared with HgCl 2 ) on metallothionein (MT) content, DNA integrity (DNA strand breaks) and catalase (CAT) in the gills and hepatopancreas, antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), in the hemolymph, gills and hepatopancreas of the portunid crab Charybdis japonica for an experiment period up to 15 d. The results indicated that MT was significantly induced after 3 d, with a positive correlation with Hg 2+ dose and time in the hepatopancreas and a negative correlation with Hg 2+ dose and time in the gills. While CAT in the hemolymph was not detected, it increased in the hepatopancreas during the entire experiment; SOD and GPx in the three tissues were stimulated after 12 h, both attained peak value and then reduced during the experimental period. Meanwhile, DNA strand breaks were all induced significantly after 12 h. These results suggested the detoxification strategies against Hg 2+ in three tissues of C. japonica.

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Dna Strand Breakage in Freshwater Mussels (Anodonta Grandis) Exposed to Lead in the Laboratory and Field

Cited by 12 publications

References 0 publications

Assessing metal, protein, and DNA profiles in Labeo rohita from the Indus River in Mianwali, Pakistan

Assessing metal, protein, and DNA profiles in Labeo rohita from the Indus River in Mianwali, Pakistan

DNA Strand Length and EROD Activity in Relation to Two Screening Measures of Genotoxic Exposure in Great Lakes Herring Gulls

Effects of mercuric chloride on antioxidant system and DNA integrity of the crab Charybdis japonica

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