DNA structure of pectate lyase I gene cloned from Erwinia carotovora.

Ito, Kazutoshi; Kobayashi, Rei; Nikaido, Naoki; Izaki, Kazuo

doi:10.1271/bbb1961.52.479

Cited by 11 publications

(8 citation statements)

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“…In addition, the pectate lyase gene ( palX ) of E. carotovora ssp. carotovora is interrupted by an IS 1240 ‐like DNA fragment encompassing tnpA and its upstream sequence [19]. This 1100 bp DNA fragment specifies an amino acid sequence that is 61% identical and 79% similar to the TnpA transposase of IS 1240 .…”

Section: Resultsmentioning

confidence: 99%

Avirulence gene D of Pseudomonas syringae pv. tomato may have undergone horizontal gene transfer

et al. 1997

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Avirulence gene D (avrD) is carried on the B‐plasmid of the plant pathogen Pseudomonas syringae pv. tomato with plasmid‐borne avrD homologs widely distributed among the Pseudomonads. We now report sequences in the soft rot pathogen Erwinia carotovora that cross‐hybridize to avrD suggesting a conserved function beyond avirulence. Alternatively, avrD may have been transferred horizontally among species: (i) DNA linked to avrD shows evidence of class II transpositions and contains a novel IS3‐related insertion sequence, and (ii) short sequences linked to avrD are similar to pathogenicity genes from a variety of unrelated pathogens. We have also identified the gene cluster that controls B‐plasmid stability.

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Section: Resultsmentioning

confidence: 99%

Avirulence gene D of Pseudomonas syringae pv. tomato may have undergone horizontal gene transfer

et al. 1997

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“…Location of translational start sites was facilitated by comparison with published sequences of pel genes from other Ec strains (25,33,34). For pe/9.5, a putative Shine-Dalgamo sequence (AGGAGA) is 7-13 bp upstream ofthe initiation codon (ATG for Nformyl methionine) (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In E. carotovora (Ec), three to four pectate lyases with similar chromatographic and hybridization properties have been described (32,52,62) and the genes coding for them have been sequenced (24,25,33,34,63). The first 22 amino acids of the excreted proteins are removed by proteolytic cleavage.…”

Section: Introductionmentioning

confidence: 99%

Site-Specific Inactivation of Bacterial Genes Involved in Plant Rotting

Antonov¹,

Dudov²,

Atanassov³

1995

Biotechnology & Biotechnological Equipment

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The soft rot bacterium Erwinia carotovora subspecies carotovora strain ECJ4 contains multiple pectate lyase genes. Genes pe/9.5 and pell0.5, coding for pectate lyases (pi 9.5 and 10.5), were cloned on a plasmid, transformed into Escherichia coli, and expressed cata~yti ca!ly active products. The isoenzymes were subcloned separate~y and sequenced. Along most of their lengths, the genes are homologous to each other and to the pelBC family of Erwinia extracellular pectate lyase genes. However, the amino acid sequence of the Pell0.5 carboxyterminus diverges markedly from the PelBC consensus. We constructed hybrid genes and their products were found to be pectolytic. The pe/9. 5 and pell 0. 5 genes were mutagenized by marker exchange mutagenesis. A double mutant, pel9.5-pell0.5-, was obtained by mobilization of pLAJJ5 into the pell0.5::kan mutant and exchange recombination by the above method. Hybridization of EcoRI digests of the mutant chromosomal DNAs with pe/9.5-, pell0.5-, kan-, and let-specific probes gave profiles consistent with single-site insertions of the kan and tet genes in the corresponding pel genes. In planta accumulation of Pe/9.5 and Pell 0. 5 mRNAs was induced within 3 to 4 hr after inoculation, reaching maxima between 6 and 12 hr with Pell 0. 5 peaking at 6 hr and Pe/9. 5 at 9 hr. The induction of the two genes was regulated differential~y and the level of their mRNAs was higher during compatible than during incompatible conditions. The three mutants, obtained here, especially the double mutant, have diminished virulence compared to the wild type.

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“…9 ) The pel III product was not secreted into the culture fluid, either, but excreted into the periplasmic space. The pell product might be accumulated in the cytoplasmic space according to the low expression of pel I in E. coli.…”

Section: Discussionmentioning

confidence: 97%