DNA super-lattice-based aptasensor for highly sensitive and selective detection of cortisol

Cantelli, Lory; Paschoalino, Waldemir J.; Kogikosky, Sergio; Pessanha, Tatiana M.; Kubota, Lauro T.

doi:10.1016/j.biosx.2022.100228

Cited by 3 publications

(1 citation statement)

References 34 publications

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“…Operational parameters are required while applying a DNA-based linker. These parameters are largely influenced by the nature of the buffer, pH, and ionic strength [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Molecularly Imprinted Polymer-Based Sensor for Electrochemical Detection of Cortisol

2022

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As a steroid hormone, cortisol has a close relationship with the stress response, and therefore, can be used as a biomarker for early detection of stress. An electrochemical immunosensor is one of the most widely used methods to detect cortisol, with antibodies as its bioreceptor. Apart from conventional laboratory-based methods, the trend for cortisol detection has seemed to be exploiting antibodies and aptamers. Both can provide satisfactory performance with high selectivity and sensitivity, but they still face issues with their short shelf life. Molecularly imprinted polymers (MIPs) have been widely used to detect macro- and micro-molecules by forming artificial antibodies as bioreceptors. MIPs are an alternative to natural antibodies, which despite demonstrating high selectivity and a low degree of cross-reactivity, often also show a high sensitivity to the environment, leading to their denaturation. MIPs can be prepared with convenient and relatively affordable fabrication processes. They also have high durability in ambient conditions, a long shelf life, and the ability to detect cortisol molecules at a concentration as low as 2 ag/mL. By collecting data from the past five years, this review summarizes the antibody and aptamer-based amperometric sensors as well as the latest developments exploiting MIPs rather than antibodies. Lastly, factors that can improve MIPs performance and are expected to be developed in the future are also explained.

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“…Operational parameters are required while applying a DNA-based linker. These parameters are largely influenced by the nature of the buffer, pH, and ionic strength [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Molecularly Imprinted Polymer-Based Sensor for Electrochemical Detection of Cortisol

2022

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Recent developments in wearable & non-wearable point-of-care biosensors for cortisol detection

Kaur

Gupta

Malhotra

2023

Expert Review of Molecular Diagnostics

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A Novel Electrochemical Aptasensor Based on a New Platform of Samarium Molybdate Flower-like Nanoparticles@Poly(pyrrole) for Non-Invasive Determination of Saliva CORTISOL

2022

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Cortisol, a famous stress biomarker, can be considered a potential predictor of cardiac diseases in humans. The presence of cortisol in saliva has encouraged researchers to design point-of-care devices for cortisol concentration in biological fluids. Here, human salivary cortisol was analyzed through a new non-invasive voltammetric aptasensor. Although cortisol is an electroactive compound, generally, the reduction in the current peak has been considered; however, this does not show a strong signal on a bare electrode surface, especially at low concentration levels. Hence, in this study, cortisol concentration was measured electrochemically and indirectly by monitoring the difference between electrochemical probe signals in the presence and absence of cortisol. A new polymeric nanocomposite of samarium molybdate flower-like nanoparticles decorated in poly(pyrrole) was electro-synthesized on the surface of a glassy carbon electrode. Then, reduced graphene oxide was cast on the surface. Finally, the cortisol aptamer was immobilized covalently on the reduced graphene oxide. This platform was used to increase the oxidation current peak of the ferricyanide solution as a probe as well as its electrocatalyst. The novel designed polymeric has the potential ability for effective immobilization of aptamers on the electrode surface without decreasing their biological activities. Additionally, it can enhance the probe electrochemical signal. The differential pulse voltammetric method (DPV) was applied as the detection technique. By optimizing the effective parameters, a determination range of 5.0 × 10−14–1.5 × 10−11 mol/L and a limit of detection of 4.5 × 10−14 mol/L were obtained. Selectivity of the proposed aptasensor relative to β-estradiol, progesterone and also prednisolone was studied as well. Finally, cortisol in a healthy human saliva sample was successfully analyzed by the proposed biosensors.

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DNA super-lattice-based aptasensor for highly sensitive and selective detection of cortisol

Cited by 3 publications

References 34 publications

Molecularly Imprinted Polymer-Based Sensor for Electrochemical Detection of Cortisol

Molecularly Imprinted Polymer-Based Sensor for Electrochemical Detection of Cortisol

Recent developments in wearable & non-wearable point-of-care biosensors for cortisol detection

A Novel Electrochemical Aptasensor Based on a New Platform of Samarium Molybdate Flower-like Nanoparticles@Poly(pyrrole) for Non-Invasive Determination of Saliva CORTISOL

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