DNA-templated quantum dots and their applications in biosensors, bioimaging, and therapy

Yang, Yeling; Mao, Guobin; Ji, Xinghu; He, Zhike

doi:10.1039/c9tb01870k

Cited by 37 publications

(22 citation statements)

References 84 publications

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“…Biomacromolecules including cell membrane structures, proteins, nucleic acids, and polysaccharides are common materials for preparing biomimetic nanomaterials. 29,30 With their specific molecular structures, 31 innate biocompatibility, and multifunctional properties, 32 these materials are widely used in drug delivery, [33][34][35] bioimaging, 36 bioengineering, 37,38 and synthesis of hybrid PTAs. In particular, biomacromolecules like protein and double-stranded DNA can be utilized as templates to synthesize PTAs with specific 3D structures and diameters, exhibiting morphology dependent enhanced tissue penetration and cellular uptake.…”

Section: Introductionmentioning

confidence: 99%

Biomacromolecule-based photo-thermal agents for tumor treatment

et al. 2021

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Section: Introductionmentioning

confidence: 99%

Biomacromolecule-based photo-thermal agents for tumor treatment

et al. 2021

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“…However, there are many disadvantages to such an approach, including cross-linking, limited reaction efficiency, and noncontrollable valence of the obtained chemical species. In addition, an increase of hydrodynamic diameter and reduction of quantum yield of QDs during the bioconjugation are inevitable drawbacks . Click chemistry is a novel method of combinatorial chemistry, which have had a significant impact on materials science, biological chemistry, and molecular science, due to its site specificity, moderate reaction conditions, high yields, and easy post-treatment. − Therefore, click reaction is a promising method for bio-functionalization of QDs.…”

Section: Introductionmentioning

confidence: 99%

Novel Method of Clickable Quantum Dot Construction for Bioorthogonal Labeling

Mao

et al. 2020

Anal. Chem.

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Bioorthogonal chemistry has been considered as a powerful tool for biomolecule labeling due to its site specificity, moderate reaction conditions, high yield, and simple post-treatment. Covalent coupling is commonly used to modify quantum dots (QDs) with bioorthogonal functional group (azide or cycloalkyne), but it has a negative effect in the decrease of QDs' quantum yield and stability and increase of QDs' hydrodynamic diameter. To overcome these disadvantages, we propose a novel method for the preparation of two kinds of clickable QDs by the strong interaction of −SH with metal ions. One system involves azide-DNA-functionalized QDs, which are used for bioconjugation with dibenzocyclooctyne (DBCO)-modified glucose oxidase (GOx) to form a GOx-QDs complex. After bioconjugation, the stability of QDs was improved, and the activity of GOx was also enhanced. The GOx-QDs complex was used for rapid detection of blood glucose by spectroscopy, naked eye, and paperbased analytical devices. The second system involves DBCO-DNA-functionalized QDs, which are used for an in situ bioorthogonal labeling of HeLa cells through metabolic oligosaccharide engineering. Therefore, these clickable QDs based on DNA functionalization can be applied for rapid and effective labeling of biomolecules of interest.

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“…These nanomaterial-based ratiometric fluorescent biosensors can be used for the precise detection of a variety of targets including pesticides, proteins, nucleic acids, and small molecules. Moreover, they have also been used for direct visualization of the target analyte with the naked eye, as well as in vitro and in vivo imaging [ 32 , 33 , 34 , 35 , 36 ]. The recent developments in the design and application of nanomaterial-based ratiometric biosensors for fluorescence sensing and cell imaging are reviewed in this section.…”

Section: Nanomaterial-based Ratiometric Fluorescent Biosensorsmentioning

confidence: 99%

Nanomaterial-Based Dual-Emission Ratiometric Fluorescent Sensors for Biosensing and Cell Imaging

et al. 2021

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Owing to the unique optophysical properties of nanomaterials and their self-calibration characteristics, nanomaterial-based (e.g., polymer dots (Pdots) quantum dots (QDs), silicon nanorods (SiNRs), and gold nanoparticle (AuNPs), etc.) ratiometric fluorescent sensors play an essential role in numerous biosensing and cell imaging applications. The dual-emission ratiometric fluorescence technique has the function of effective internal referencing, thereby avoiding the influence of various analyte-independent confounding factors. The sensitivity and precision of the detection can therefore be greatly improved. In this review, the recent progress in nanomaterial-based dual-emission ratiometric fluorescent biosensors is systematically summarized. First, we introduce two general design approaches for dual-emission ratiometric fluorescent sensors, involving ratiometric fluorescence with changes of one response signal and two reversible signals. Then, some recent typical examples of nanomaterial-based dual-emission ratiometric fluorescent biosensors are illustrated in detail. Finally, probable challenges and future outlooks for dual-emission ratiometric fluorescent nanosensors for biosensing and cell imaging are rationally discussed.

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DNA-templated quantum dots and their applications in biosensors, bioimaging, and therapy

Abstract: In this review, we summarize the current progress on phosphorothioated-DNA functionalized QDs for applications in biosensors, bioimaging, and therapy.

Cited by 37 publications

References 84 publications

Biomacromolecule-based photo-thermal agents for tumor treatment

Biomacromolecule-based photo-thermal agents for tumor treatment

Novel Method of Clickable Quantum Dot Construction for Bioorthogonal Labeling

Nanomaterial-Based Dual-Emission Ratiometric Fluorescent Sensors for Biosensing and Cell Imaging

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