Dna Topoisomerases

Wang, James C.; Gumport, Richard I.; Javaherian, Kashayar; Kirkegaard, Karla; Klevan, Leonard; Kotewicz, Michael L.; Tse, Yuk-Ching

doi:10.1016/b978-0-12-048850-6.50070-3

Cited by 317 publications

(470 citation statements)

References 28 publications

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“…Our laboratory is now actively conducting work to elucidate the particular mechanism(s) involved in CD26/DPPIV-associated enhancement in topoisomerase II a protein level and enzyme activity. Essential for cellular proliferation as a key regulator of mitosis, DNA topoisomerase II enzyme catalyses many types of interconversions between different DNA topological isomers (Wang, 1996). Eukaryotes have two isoforms of topoisomerase II, a and b, which are coded by two distinct genes (Drake et al, 1989).…”

Section: Discussionmentioning

confidence: 99%

Effect of CD26/dipeptidyl peptidase IV on Jurkat sensitivity to G2/M arrest induced by topoisomerase II inhibitors

et al. 2003

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CD26/dipeptidyl peptidase IV (DPPIV) is a surface antigen with multiple functions, including a role in T-cell activation and the development of certain human cancers. We previously demonstrated that CD26/DPPIV enhanced sensitivity of Jurkat cells to doxorubicin. We now show that expression of CD26/DPPIV enhanced sensitivity of CD26 Jurkat transfectants to G 2 -M arrest mediated by the antineoplastic agent etoposide. The increased sensitivity to etoposide-induced G 2 -M arrest was associated with disruption of cell cycle-related events, including hyperphosphorylation of p34 cdc2 kinase, change in cdc25C expression and phosphorylation, and alteration in cyclin B1 expression. CD26/DPPIV-associated enhancement of doxorubicin and etoposideinduced G 2 -M arrest was also observed in serum-free media, suggesting an effect of CD26 on cell-derived processes rather than serum-derived factors. Importantly, our work elucidated a potential mechanism for the enhanced susceptibility of CD26-expressing Jurkat cells to the topoisomerase II inhibitors by demonstrating that CD26/DPPIV surface expression was associated with increased topoisomerase II a levels and enhanced enzyme activity. Besides being the first to show a functional association between the multifaceted molecule CD26 and the key cellular protein topoisomerase II a, our studies provide additional evidence of a potential role for CD26 in the treatment of selected malignancies.

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Section: Discussionmentioning

confidence: 99%

Effect of CD26/dipeptidyl peptidase IV on Jurkat sensitivity to G2/M arrest induced by topoisomerase II inhibitors

et al. 2003

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“…1 Several recent papers review the cellular roles of Topo1. [1][2][3][4] A role in chromosome condensation has been proposed for Topo1, 5 and this enzyme also supports the elongation step of DNA replication, like other type IB enzymes. Concerning gene expression mechanisms, Topo1 is involved in solving topological problems associated with transcription, and it has been shown to be present in actively transcribed regions.…”

Section: Introductionmentioning

confidence: 53%

“…Concerning gene expression mechanisms, Topo1 is involved in solving topological problems associated with transcription, and it has been shown to be present in actively transcribed regions. 2,4 In addition, Topo1 interacts with transcription factors and participates in the construction of an active TFIID-TFIIA protein complex in a DNA strand breakageindependent manner. 6,7 Topo1 seems to enhance the response to transcription activators in the case of TATA boxes present in promoters and represses basal transcription in the absence of activators.…”

Section: Introductionmentioning

confidence: 99%

“…6,7 Topo1 seems to enhance the response to transcription activators in the case of TATA boxes present in promoters and represses basal transcription in the absence of activators. 4 Finally, Topo1 contributes to RNA splicing. 8,9 Regarding the Topo1 subcellular localization, a nuclear localization signal is present in its N-terminal region 10 and a direct interaction with nucleolin, a major nucleolar protein, has been shown by Bharti et al 11 thus indicating a probable nuclear localization.…”

Section: Introductionmentioning

confidence: 99%

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The topoisomerase 1-interacting protein BTBD1 is essential for muscle cell differentiation

et al. 2004

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DNA topoisomerase I (Topo1) contributes to vital biological functions, but its regulation is not clearly understood. The BTBD1 protein was recently cloned on the basis of its interaction with the core domain of Topo1 and is expressed particularly in skeletal muscle. To determine BTBD1 functions in this tissue, the in vitro model used was the C2C12 mouse muscle cell line, which expresses BTBD1 mainly after myotube differentiation. We studied the effects of a stably overexpressed BTBD1 protein truncated of the 108 N-terminal amino-acid residues and harbouring a C-terminal FLAG tag (D-BTBD1). The proliferation speed of D-BTBD1 C2C12 cells was significantly decreased and no myogenic differentiation was observed, although these cells maintained their capacity to enter adipocyte differentiation. These alterations could be related to Topo1 deregulation. This hypothesis is further supported by the decrease in nuclear Topo1 content in D-BTBTD1 proliferative C2C12 cells and the switch from the main peripheral nuclear localization of Topo1 to a mainly nuclear diffuse localization in D-BTBTD1 C2C12 cells. Finally, this study demonstrated that BTBD1 is essential for myogenic differentiation.

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“…Several of the most active antineoplastic agents paralyse this enzyme by stabilising a complex between the enzyme and the DNA strands manipulated by it in the course of its normal function (Wang, 1985;Liu, 1989;Zwelling, 1989). This stabilised complex poisons the cell by initiating an apoptotic cell death pathway whose biochemistry is not completely understood at present.…”

mentioning

confidence: 99%

Effect of transfection of a Drosophila topoisomerase II gene into a human brain tumour cell line intrinsically resistant to etoposide

Asano

Zwelling²,

An³

et al. 1996

Br J Cancer

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Dna Topoisomerases

Cited by 317 publications

References 28 publications

Effect of CD26/dipeptidyl peptidase IV on Jurkat sensitivity to G2/M arrest induced by topoisomerase II inhibitors

Effect of CD26/dipeptidyl peptidase IV on Jurkat sensitivity to G2/M arrest induced by topoisomerase II inhibitors

The topoisomerase 1-interacting protein BTBD1 is essential for muscle cell differentiation

Effect of transfection of a Drosophila topoisomerase II gene into a human brain tumour cell line intrinsically resistant to etoposide

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