DNA transfer when using gloves in burglary simulations

Carrara, L.; Hicks, Tacha; Samie, Lydie; Taroni, Franco; Castella, Vincent

doi:10.1016/j.fsigen.2022.102823

Cited by 7 publications

(5 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The occurrence of mixed profiles despite the thorough item cleaning and the use of a single DNA donor could be explained by transferring foreign DNA from the donor's hands to the glass, as also described by Daly et al [44] or Carrara et al [61]. In this study, the donor was instructed not to wash hands directly before applying the DNA, so foreign DNA may have been transferred to the plate by touching door handles, keys, or other objects, such as computer accessories, office documents, or cell phones.…”

Section: Discussionmentioning

confidence: 88%

Collecting touch DNA from glass surfaces using different sampling solutions and volumes: Immediate and storage effects on genetic STR analysis

Schulte,

Rittiner,

Seiberle

et al. 2023

Journal of Forensic Sciences

View full text Add to dashboard Cite

Touch DNA has become increasingly important evidence in todays' forensic casework. However, due to its invisible nature and typically minute amounts of DNA, the collection of biological material from touched objects remains a particular challenge that underscores the importance of the best collection methods for maximum recovery efficiency. So far, swabs moistened with water are often utilized in forensic crime scene investigations for touch DNA sampling, even though an aqueous solution provokes osmosis, endangering the cell's integrity. The aim of the research presented here was to systematically determine whether DNA recovery from touched glass items can be significantly increased by varying swabbing solutions and volumes compared with water‐moistened swabs and dry swabbing. A second objective was to investigate the possible effects of storage of swab solutions prior to genetic analysis on DNA yield and profile quality when stored for 3 and 12 months, as is often the case with crime scene samples. Overall, the results indicate that adapting volumes of the sampling solutions had no significant effect on DNA yield, while the detergent‐based solutions performed better than water and dry removal, with the SDS reagent yielding statistically significant results. Further, stored samples showed an increase in degradation indices for all solutions tested, but no deterioration in DNA content and profile quality, allowing for unrestricted processing of touch DNA samples stored for at least 12 months. One further finding was a strong intraindividual change in DNA amounts observed over the 23 deposition days which may be related to the donor's menstrual cycle.

show abstract

Section: Discussionmentioning

confidence: 88%

Collecting touch DNA from glass surfaces using different sampling solutions and volumes: Immediate and storage effects on genetic STR analysis

Schulte,

Rittiner,

Seiberle

et al. 2023

Journal of Forensic Sciences

View full text Add to dashboard Cite

show abstract

“…Carrara et al [7] recently performed an experiment to investigate the possibility of generating an indirect transfer in burglary simulations, confirming this alarming event.…”

Section: Main Findingsmentioning

confidence: 81%

“…Carrara et al, 2023, Switzerland [7] This study investigated a secondary transfer mediated by gloves during simulated burglary simulations.…”

Section: Szkuta Et Al 2019mentioning

confidence: 99%

“…The activity level of DNA transfer in criminal cases is of great importance as it has been observed that not only direct transfer of DNA (primary) can be found at a crime scene but also indirect transfer (secondary) from unrelated individuals through potential vectors such as objects or persons. Numerous studies have described this possibility, highlighting the crucial role that DNA transfer can play in criminal investigations [7][8][9][10]. A seminal paper on the possibility of indirect DNA transfer was written by van Oorschot and Jones in 1997 [11].…”

Section: Introductionmentioning

confidence: 99%

“…Cleanliness can affect the presence of contaminants or inhibitors that can degrade or interfere with DNA analysis[7,25,26,[31][32][33]37,42,45,46,50,51,53,55,57,58,60,64,66,69].• Trace type: This refers to whether the trace is fresh or dry, visible or invisible, singlesource or mixed-source. Fresh traces are more likely to contain viable cells that can be amplified by PCR than dry traces.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Indirect DNA Transfer and Forensic Implications: A Literature Review

Sessa,

Pomara,

Esposito

et al. 2023

Genes

View full text Add to dashboard Cite

Progress in DNA profiling techniques has made it possible to detect even the minimum amount of DNA at a crime scene (i.e., a complete DNA profile can be produced using as little as 100 pg of DNA, equivalent to only 15–20 human cells), leading to new defense strategies. While the evidence of a DNA trace is seldom challenged in court by a defendant’s legal team, concerns are often raised about how the DNA was transferred to the location of the crime. This review aims to provide an up-to-date overview of the experimental work carried out focusing on indirect DNA transfer, analyzing each selected paper, the experimental method, the sampling technique, the extraction protocol, and the main results. Scopus and Web of Science databases were used as the search engines, including 49 papers. Based on the results of this review, one of the factors that influence secondary transfer is the amount of DNA shed by different individuals. Another factor is the type and duration of contact between individuals or objects (generally, more intimate or prolonged contact results in more DNA transfer). A third factor is the nature and quality of the DNA source. However, there are exceptions and variations depending on individual characteristics and environmental conditions. Considering that secondary transfer depends on multiple factors that interact with each other in unpredictable ways, it should be considered a complex and dynamic phenomenon that can affect forensic investigation in various ways, for example, placing a subject at a crime scene who has never been there. Correct methods and protocols are required to detect and prevent secondary transfer from compromising forensic evidence, as well as the correct interpretation through Bayesian networks. In this context, the definition of well-designed experimental studies combined with the use of new forensic techniques could improve our knowledge in this challenging field, reinforcing the value of DNA evidence in criminal trials.

show abstract

Hebelwerkzeuge als Spurenträger

Hollenbach,

Schlegel,

Cappel-Hoffmann

et al. 2024

Rechtsmedizin

View full text Add to dashboard Cite

ZusammenfassungWerkzeuge als Spurenträger gewinnen zunehmendes Interesse in der forensischen DNA-Analyse. Im Untersuchungsgut des Homburger Instituts ist eine deutliche Zunahme von Hebelspuren zu verzeichnen. Vor diesem Hintergrund verfolgt die vorliegende Studie das Ziel, ein rational begründetes Konzept für die Tatortarbeit zu entwickeln.Im ersten Teilschritt wurde der DNA-Gehalt entsprechender Spuren systematisch experimentell ermittelt: Zwei Versuchspersonen setzten mit 6 verschiedenen Hebelwerkzeugen (4 Schraubendreher, 2 Nageleisen) an 3 Fenstern verschiedener Materialart (Holz, Aluminium, Kunststoff) in jeweils 24 definierten Arealen Hebelspuren. Fenster und Werkzeuge wurden mit Microbac forte (Hartmann) bzw. DNA Exitus (Applichem) gereinigt. Anschließend wurden an den Werkzeugen 3‑mal täglich, an 3 aufeinanderfolgenden Tagen unter leichtem Druck 10 aufeinanderfolgende Reibbewegungen durchgeführt. Die gefertigten Abriebe wurden standardisiert analysiert (DNA-Extraktion mit EZ1 DNA-Investigator Kit, Investigator Lyse/Spin-Basket Kit, Fa. Qiagen; Quantifizierung mittels Real-Time PCR, Investigator Quantiplex Pro Kit, Fa. Qiagen; Multiplex-PCR mit PowerPlex® ESX 17 Kit, Fa. Promega, und Kapillarelektrophorese mittels 3500 Genetic Analyzer von Applied Biosystems und Gene Mapper ID-X Software). Bei der Untersuchung von 72 Spuren wurde an einer mit einem Schraubendreher an einem Kunststofffenster gesetzten Hebelspur eine DNA in einer Konzentration von 22 pg/µl nachgewiesen und ein homogenes DNA-Profil generiert.Anschließend wurde in einer retrospektiven Analyse empirisch verifiziert, inwiefern die experimentellen Daten eine Entsprechung in der tatsächlichen ermittlungsseitigen Fallarbeit/Spurensicherung gefunden haben. Hierzu wurden die in einem Dreijahreszeitraum vor der experimentellen Studie untersuchten 90 Hebelspuren bezüglich des Anteils der nachgewiesenen DNA-Profile ausgewertet. Dabei fanden sich 9 auswertbare Mischspuren sowie ein vollständig homogenes Profilmuster, das anhand des Ermittlungsansatzes einem Tatverdächtigen zugeordnet werden konnte. Diese Daten sollten im konkreten Ermittlungsfall bei einer kriminalistischen Abwägung zwischen Aufwand und Nutzen einer Spurenuntersuchung an der eigentlichen Hebelmarke Berücksichtigung finden.

show abstract

DNA transfer when using gloves in burglary simulations

Cited by 7 publications

References 51 publications

Collecting touch DNA from glass surfaces using different sampling solutions and volumes: Immediate and storage effects on genetic STR analysis

Collecting touch DNA from glass surfaces using different sampling solutions and volumes: Immediate and storage effects on genetic STR analysis

Indirect DNA Transfer and Forensic Implications: A Literature Review

Hebelwerkzeuge als Spurenträger

Contact Info

Product

Resources

About