DNA typing for HLA‐DR, and ‐DP alleles in a Chinese population using the polymerase chain reaction (PCR) and oligonucleotide probes

Abstract

We have determined alleles of HLA-DRB1, DRB3, DRB5, DQA1, DQB1, and DPB1 loci in 91 unrelated healthy individuals from North China. Group-specific PCR primers were employed for the analysis of subsets of DR1, DR2, DR4, DRw52, and DPB. With allele-specific probes, 22 DRB1, 8 DQA1, 13 DQB1, and 12 DPB1 alleles were found in this panel. Allele frequencies showed that 25.3% of the subjects had DR7 and 26.4% had DR9, only 5.5% had DRB1*0301 (DRw17). In the DR4 group, DRB1*0405 (Dw15, 8.8%) and 0406 (KT2, 9.9%) were the most prevalent alleles. DRB1*0404 (Dw14.1), 0407 (Dw13.2) and 0408 (Dw14.2) were absent and the other alleles of the DR4 group were rare. The most common DRw6 subset was DRB1*1401 (8.8%). DRB1*0802 and 0803 were present (2.2%, 6.6%), and DRB1*0801 was not found. Associations with DQA1 and DQB1 were generally similar to those found in other populations. DPB1*0501 was the most frequent (60.2%) allele at the DPB1 locus. Overall our study shows that the distribution of class II alleles in a population from Mainland China is quite different from other ethnic groups. The high frequency of the KT2 subset of DR4. (DRB1*0406) and of DPB1*0501 are the most striking features found. A new type of DR4 was determined in one subject. It was like DR4-Dw15 (DRB1*0405) but, according to our hybridization patterns, it encoded valine instead of glycine in position 86. It is now called DRB1*0410.

Differential Distribution of HLA-DQβ/DRβ Epitopes in the Two Forms of Guillain-Barré Syndrome, Acute Motor Axonal Neuropathy and Acute Inflammatory Demyelinating Polyneuropathy (AIDP): Identification of DQβ Epitopes Associated with Susceptibility to and Protection from AIDP

“…The total number of subjects typed and alleles identified are indicated under each category in Tables I-III. The frequencies of the different alleles of the three loci in the control population are in general agreement with previously published HLA class II typing in the same population of northern China (37).…”

“…It should be noted that the linkage disequilibrium between the DR and DQ epitopes observed is partially due to the linkage disequilibrium of the DRB1*0405 with DQB1*0401 alleles ( p ϭ 0.0001; odds ratio, 333), both of which include the DR␤ E 9 V 11 H 13 and DQ␤ ED 70 -71 epitopes, respectively. Linkage disequilibrium involving the same alleles has also been reported in the same population of northern Chinese by Gao et al (37).…”

Childhood Narcolepsy in North China

“…All these are higher than that in the greater Chinese community. 16,17 In our group, some findings are in contrast to that found in other Asians, even of Hong Kong Chinese, in whom there was reported a 100% association of HLADR 2 and HLADQw6 with narcolepsy. 9,15 We found one child with sleepiness and cataplexy (but without SOREM), who was HLADR 2 and HLADQw6 negative.…”

Ventilatory chemoresponsiveness, narcolepsy–cataplexy and human leukocyte antigen DQB1*0602 status

“…While DQB1*0602 is an allele present in all patients with narcolepsy and cataplexy, it is not a disease-defining HLA maker, as it is present in the healthy populations [23]. Few studies hint at potential relationships between immune markers and hypoxia.…”