2013
DOI: 10.1099/mic.0.062711-0
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DNA unmasked in the red rain cells of Kerala

Abstract: Extraordinary claims have been made for the biological properties of the red rain cells of Kerala, including a suggestion that they lack DNA. We have investigated the fluorescence properties of red rain cells, and the solubility of the red pigment in a variety of solvents. Extraction of the pigment with DMSO allowed successful demonstration of DNA using DAPI staining. Cellular impermeability to staining reagents due to the red pigment is the likely explanation for the failure of previous efforts to demonstrate… Show more

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Cited by 5 publications
(2 citation statements)
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“…Later we argued that the result of DAPI staining is not conclusive because of the continuous auto-fluorescence of the red cells, when its outer red pigment was removed [32]. The wide auto fluorescence behavior of the depigmented red cells has been described by Louis & Kumar [32] and Gangappa & Hogg [31]. Hence the blue fluorescence is not due to DNA rather due to autofluorescence of the red cells.…”
Section: Evidences For Unusual Nature and Extraterrestrial Origin Of mentioning
confidence: 88%
See 1 more Smart Citation
“…Later we argued that the result of DAPI staining is not conclusive because of the continuous auto-fluorescence of the red cells, when its outer red pigment was removed [32]. The wide auto fluorescence behavior of the depigmented red cells has been described by Louis & Kumar [32] and Gangappa & Hogg [31]. Hence the blue fluorescence is not due to DNA rather due to autofluorescence of the red cells.…”
Section: Evidences For Unusual Nature and Extraterrestrial Origin Of mentioning
confidence: 88%
“…Reports of DNA detection using DAPI staining by Microscopic Probe technique after pigment removal by DMSO showed positive results [31]. Later we argued that the result of DAPI staining is not conclusive because of the continuous auto-fluorescence of the red cells, when its outer red pigment was removed [32].…”
Section: Evidences For Unusual Nature and Extraterrestrial Origin Of mentioning
confidence: 90%