1977
DOI: 10.1111/j.1432-1033.1977.tb11781.x
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DNA Unwinding Enzyme II of Escherichia coli

Abstract: The DNA-stimulated 75000-M, ATPase described in the preceding paper is shown to be a further catalytic DNA unwinding principle (DNA unwinding enzyme 11) made in Escherichia coli cells (the first being the 180000-M, ATPase of the cells: DNA unwinding enzyme I). Unwinding depends, strictly, on the supply of ATP. It occurs only under conditions permitting ATP dephosphorylation and it proceeds as long as enzyme molecules are permitted to enter the enzyme . DNA complex. The enzyme binds specifically to single-stran… Show more

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Cited by 76 publications
(23 citation statements)
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“…When helicase reactions were carried out in the presence of varying amounts of SSB protein (0.625-20 nM), similar inhibition of UvrD activity was observed on both the non-damaged and damaged substrate in the presence of SSB protein (data not shown). Our results are consistent with most of the previously published work, demonstrating an inhibition of UvrD helicase activity in the presence of SSB protein (40,41).…”
Section: Resultssupporting
confidence: 93%
“…When helicase reactions were carried out in the presence of varying amounts of SSB protein (0.625-20 nM), similar inhibition of UvrD activity was observed on both the non-damaged and damaged substrate in the presence of SSB protein (data not shown). Our results are consistent with most of the previously published work, demonstrating an inhibition of UvrD helicase activity in the presence of SSB protein (40,41).…”
Section: Resultssupporting
confidence: 93%
“…Neither ATPase of the six chromosome-encoded proteins with helicase activity thus far identified in E. coli (29)(30)(31)(32)(33)(34) by biochemical properties (32)(33)(34) or genetic map positions (1,(35)(36)(37)(38). Incidentally, our RecQ preparation did not exhibit, as far as examined, any DNase and DNA topoisomerase activities in the presence or absence of ATP (our unpublished results).…”
Section: Discussionmentioning
confidence: 99%
“…The uvrD gene product has been shown to be DNA helicase II (103)(104)(105), an activity that catalyzes the ATP-dependent unwinding of duplex DNA (106,107) . E. coli SSB binds tightly and cooperatively to single-stranded DNA and stimulates the action of E. coli DNA polymerases II and III (reviewed in Ref.…”
Section: Proteins Required Fo R Dam-directed Mismatch Repairmentioning
confidence: 99%