DNA Vaccine Mediated by Rambutan‐Like Mesoporous Silica Nanoparticles

Song, Hao; Yang, Yannan; Tang, Jie; Gu, Zhengying; Wang, Yue; Zhang, Min; Yu, Chengzhong

doi:10.1002/adtp.201900154

Cited by 19 publications

(18 citation statements)

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“…report the development of a MSN‐based DNA vaccine using rambutan‐like MSNs as both gene vector and adjuvant. [ 143 ] The rambutan‐like MSNs have been developed through co‐polymerization of resorcinol‐formaldehyde (RF) resin and silica to show unique spiky nanotopography, further modified with cationic PEI ( Figure ) which have previously been shown to show superior pDNA delivery and effective protection of gene from nuclease degradation. [ 144 ] Authors show that rambutan‐like MSN containing ovalbumin (OVA)‐encoding pDNA (pDNA‐OVA) vaccine enhances antigen‐specific IgG production and dendritic cell maturation with enhanced CD80 and CD86 expression, and the MSNs improved antigen‐specific IgG antibody, cytokine production of IFN‐ γ , and increased CD8+ T cell activation in mice.…”

Section: Nanoparticles Are An Advantageous Methods For Delivering Nucleic Acid Vaccinesmentioning

confidence: 99%

“…[ 144 ] Authors show that rambutan‐like MSN containing ovalbumin (OVA)‐encoding pDNA (pDNA‐OVA) vaccine enhances antigen‐specific IgG production and dendritic cell maturation with enhanced CD80 and CD86 expression, and the MSNs improved antigen‐specific IgG antibody, cytokine production of IFN‐ γ , and increased CD8+ T cell activation in mice. [ 143 ] Furthermore, the immune response of their MSN‐based DNA vaccine outperformed a commercially available transfection agent, in vivo‐jetPEI.…”

Section: Nanoparticles Are An Advantageous Methods For Delivering Nucleic Acid Vaccinesmentioning

confidence: 99%

“…[147] Furthermore, many inorganic NPs used for nucleic acid delivery are proof-of-concept studies which necessarily imply that further ) Ram-MSNs-PEI corresponding particle size distribution determined by DLS D) nitrogen sorption isotherm Reproduced with permission. [143] Copyright 2019, Wiley-VCH GmbH.…”

Section: Inorganic Nanoparticlesmentioning

confidence: 99%

“…Rambutan-like mesoporous silica nanoparticles which show superior pDNA adsorption. A) TEM images of B) Ram-MSNs and C) Ram-MSNs-PEI corresponding particle size distribution determined by DLS D) nitrogen sorption isotherm Reproduced with permission [143]. Copyright 2019, Wiley-VCH GmbH.…”

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confidence: 99%

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Next‐Generation Vaccines: Nanoparticle‐Mediated DNA and mRNA Delivery

Gao

et al. 2021

Adv Healthcare Materials

184

139

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Nucleic acid vaccines are a method of immunization aiming to elicit immune responses akin to live attenuated vaccines. In this method, DNA or messenger RNA (mRNA) sequences are delivered to the body to generate proteins, which mimic disease antigens to stimulate the immune response. Advantages of nucleic acid vaccines include stimulation of both cell-mediated and humoral immunity, ease of design, rapid adaptability to changing pathogen strains, and customizable multiantigen vaccines. To combat the SARS-CoV-2 pandemic, and many other diseases, nucleic acid vaccines appear to be a promising method. However, aid is needed in delivering the fragile DNA/mRNA payload. Many delivery strategies have been developed to elicit effective immune stimulation, yet no nucleic acid vaccine has been FDA-approved for human use. Nanoparticles (NPs) are one of the top candidates to mediate successful DNA/mRNA vaccine delivery due to their unique properties, including unlimited possibilities for formulations, protective capacity, simultaneous loading, and delivery potential of multiple DNA/mRNA vaccines. This review will summarize the many varieties of novel NP formulations for DNA and mRNA vaccine delivery as well as give the reader a brief synopsis of NP vaccine clinical trials. Finally, the future perspectives and challenges for NP-mediated nucleic acid vaccines will be explored.

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Section: Nanoparticles Are An Advantageous Methods For Delivering Nucleic Acid Vaccinesmentioning

confidence: 99%

Section: Nanoparticles Are An Advantageous Methods For Delivering Nucleic Acid Vaccinesmentioning

confidence: 99%

Section: Inorganic Nanoparticlesmentioning

confidence: 99%

mentioning

confidence: 99%

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Next‐Generation Vaccines: Nanoparticle‐Mediated DNA and mRNA Delivery

Gao

et al. 2021

Adv Healthcare Materials

184

139

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“…Mice were euthanized to collect major organs and lymph nodes for visualization by IVIS imaging system. 101…”

Section: In Vivo Studiesmentioning

confidence: 99%

Designed synthesis of silica based nanocarriers for mRNA delivery

Wang¹

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In recent decades, introducing messenger RNA (mRNA) into cells has garnered intense interest for diverse applications. Limited by the short half-life and cell-membrane impermeability of mRNA, the success requires delivery solutions. With extensive efforts devoted in the nanomaterials based mRNA delivery platform, current design strategies for mRNA delivery systems primarily focus on mRNA binding, intracellular delivery and protection from degradation, but few provide the functionality to directly regulate mRNA translation. Besides, current delivery technology has limited delivery efficiency in hard-to-transfect cells (e.g., macrophages). Thus, new strategies are highly desired to exploit the multifunctional nanoparticles as both transfection agents and translation regulators for enhanced mRNA delivery with improved performance.Inspired by our recent breakthroughs in mesoporous silica nanoparticles (MSNs) synthesis, combined with the fundamental regulatory mechanism and intracellular environment of specific cells, this thesis focuses on the synthesis of functional dendritic mesoporous silica nanoparticles (DMSNs) designed for enhanced mRNA delivery. A facile, water phase based synthetic system was developed for the fabrication of large pore DMSNs with controlled structure and composition. Through the systematic study of the structural evolution of dahlia-like DMSNs to pomegranate-like MSNs via a micelle filling mechanism, the heterogeneous porous structure of DMSNs was reported for the first time for delivery of two positively charged cargoes. By comparing tetrasulfide bond bridged dendritic mesoporous organosilica nanoparticles (DMONs) with inorganic DMSNs with keeping other parameters the same, the composition contribution on intracellular glutathione (GSH) depletion, translation pathway regulation and mRNA delivery performance in antigen presenting cells were studied for the first time.Moreover, zeolitic imidazolate framework-8 (ZIF-8) was introduced to grow inside the mesopores of DMONs. The combined effect of ZIF-8 and tetrasulfide bond on the intracellular regulation of GSH and mRNA delivery was studied, providing new understandings in controllable synthesis of advanced materials as mRNA delivery vehicles.In the first experimental chapter, we report a unique dynamic structural transition from large pore sized DMSNs with the dahlia-like morphology to pomegranate-like MSNs with small mesopores.The silica-coated micelle composites were revealed to contribute to the kinetic structural evolution and heterogeneity of DMSNs. The structural evolution window was finely controlled by several parameters, including reaction time, reaction temperature, molar ratio of anionic micelle penetration agents to cationic surfactant, type of anions and amount of silica precursor. The advantage of the heterogeneous DMSNs with dual mesopores was further demonstrated for the co-delivery of two positively charge molecules, ribonuclease (RNase A, pKa= 9.6, Mw = 13.7 kDa, a model therapeutic I acknowledge that copyright of all material...

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Mesoporous Silica Nanoparticles as an Ideal Platform for Cancer Immunotherapy: Recent Advances and Future Directions.

Godakhindi,

Tarannum,

Dam

et al. 2024

Adv Healthcare Materials

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Cancer immunotherapy has recently transformed the traditional approaches against various cancer malignancies. Immunotherapy includes systemic and local treatments to enhance immune responses against cancer; and involves strategies such as immune checkpoints, cancer vaccines, immune modulatory agents, mimetic antigen‐presenting cells, and adoptive cell therapy. Despite promising results, these approaches still suffer from several limitations including a lack of precise delivery of immune‐modulatory agents to the target cells, off‐target toxicity, among others that can be overcome using nanotechnology. Mesoporous silica nanoparticles (MSNs) have been investigated to improve various aspects of cancer immunotherapy attributed to the advantageous structural features of this nanomaterial. MSNs can be engineered to alter their properties such as size, shape, porosity, surface functionality, and adjuvanticity. This review explores the immunological properties of MSNs; and the use of MSNs as delivery vehicles for immune‐adjuvants, vaccines, and mimetic antigen‐presenting cells (APCs). The review also details the current strategies to remodel the tumor microenvironment to positively reciprocate towards the anti‐tumor immune cells and the use of MSNs for immunotherapy in combination with other anti‐tumor therapies including photodynamic/thermal therapies to enhance the therapeutic effect against cancer. Lastly, the present demands and future scenarios for the use of MSNs for cancer immunotherapy are discussed.This article is protected by copyright. All rights reserved

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DNA Vaccine Mediated by Rambutan‐Like Mesoporous Silica Nanoparticles

Abstract: This article is protected by copyright. All rights reserved. 2 platform with outstanding immune responses, which provides useful guidance for the design of effective nanomaterial-based vaccines.

Cited by 19 publications

References 47 publications

Next‐Generation Vaccines: Nanoparticle‐Mediated DNA and mRNA Delivery

Next‐Generation Vaccines: Nanoparticle‐Mediated DNA and mRNA Delivery

Designed synthesis of silica based nanocarriers for mRNA delivery

Mesoporous Silica Nanoparticles as an Ideal Platform for Cancer Immunotherapy: Recent Advances and Future Directions.

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