DNA variations in regenerated plants of pea (Pisum sativum L.)

Cecchini, Edi; Natali, Lucia; Cavallini, Andrea; Durante, M.

doi:10.1007/bf00227399

Cited by 55 publications

(21 citation statements)

References 31 publications

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“…Long term multiplication may lead to somaclonal or epigenetic variations in the micropropagated plants questioning the fidelity of their clonal nature (Cecchini et al 1992;Rani and Raina 1998). Therefore, it is important to first establish the suitability of the micropropagation protocol developed with respect to the production of genetically identical and stable plants.…”

Section: Histologymentioning

confidence: 99%

RETRACTED ARTICLE: Plant regeneration in Chlorophytum borivilianum Sant. et Fernand. from embryogenic callus and cell suspension culture and assessment of genetic fidelity of plants derived through somatic embryogenesis

Rizvi

Kukreja

Bisht

2012

Physiol Mol Biol Plants

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Efficient in vitro propagation of medicinally important endangered plant C. borivilianum has been achieved through somatic embryogenesis. Solid embryogenic medium [Murashige and Skoog medium containing 1.79 mM NH 4 NO 3 , 10.72 mM KNO 3, 1.13 μM 2,4-dichlorophenoxyacetic acid, 7.38 μM 2-isopentenyladenine and 0.76 mM proline] supplemented with polyethylene glycol and sucrose (3 % each), exhibited 1.88-fold increase in embryo maturation compared to embryogenic medium containing 3 % sucrose. Liquid embryogenic medium supported better somatic embryo production and maturation. Highest total (79) and mature (cotyledonary stage) somatic embryos (38) as well as highest germination (57.5 %) was observed at inoculum density of 0.4 g/40 ml of liquid medium. 5.86 pH level exhibited optimal growth, maturation and germination of somatic embryos. Random amplified polymorphic DNA (RAPD) analysis of C. borivilianum plants regenerated through somatic embryogenesis revealed that they were genetically similar to the mother plant. The protocol established in the present study can be used for rapid mass multiplication of C. borivilianum in bioreactor employing liquid medium.

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Section: Histologymentioning

confidence: 99%

RETRACTED ARTICLE: Plant regeneration in Chlorophytum borivilianum Sant. et Fernand. from embryogenic callus and cell suspension culture and assessment of genetic fidelity of plants derived through somatic embryogenesis

Rizvi

Kukreja

Bisht

2012

Physiol Mol Biol Plants

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“…Similarly, 133 amplification products generated by 12 arbitrary 10-base primers and six oligonucleotide sequences failed to detect polymorphism among micropropagated plants. Mitochondrial, nuclear rDNA, oligonucleotide DNA probes/primers, RAPD fingerprinting, and nuclear genome size estimation singly or in combination have been effectively used in many studies to detect tissue-culture-induced variation (Breiman et al 1987;Brettel et al 1986;Brown et al 1993;Cecchini et al 1992;Deumling and Clermont 1989;Hartman et al 1989;Isabel et al 1993;Poulsen et al 1993;Rani et al 1995;Rode et al 1987;Shimron-Abarbanell and Breiman 1991;Shirzadegan et al 1991).…”

Section: Pcr-based Oligonucleotide Fingerprintingmentioning

confidence: 99%

“…In the study presented here, we have screened enhancedaxillary-branching-derived Eucalyptus tereticornis and E. camaldulensis plants by RFLP, RAPD, and oligonucleotide fingerprinting markers to assess their genetic fidelity, and to enquire into the applicability of the marker(s) for rapid appraisal of tissue-culture-derived eucalypt plants. In addition, cytophotometric measurements from root and shoot tip apices, collected at various cultural passages and after transfer to field conditions, were also compared, since the genetic changes brought about by amplification or loss of DNA sequences have now been shown to be affected by tissue culture stress (Cecchini et al 1992;Cullis and Cleary 1986;Deumling and Clermont 1989;Landsmann and Uhrig 1985). The results also provide novel insights into the genetic differences between two (E. tereticornis and E. camaldulensis) of the ten most economically important and widely planted Eucalyptus species.…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis of enhanced-axillary-branching-derived Eucalyptus tereticornis Smith and E. camaldulensis Dehn. plants

Rani

Raina²

1998

Plant Cell Reports

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In a culture method for enhanced axillary branching functional plants of Eucalyptus tereticornis and E. camaldulensis are efficiently regenerated. To assess the genetic integrity among the regenerants, we employed multiple analytical tools including cytochemical and molecular assays. The 2C DNA amounts were estimated in the meristematic zones of root and shoot tips of 250 micropropagated plants, collected at various cycles of tissue culture from multiplication to field transfer, and compared to the corresponding mother plants. The culture conditions did not induce amplification or deletion of DNA sequences, nor were there drastic change(s) in chromosome number, since all the micropropagated plants of E. tereticornis (1.2 pg) and E. camaldulensis (1.4 pg) maintained the same DNA amounts as the mother plant. Total DNA of 46 micropropagated and mother plants digested with eight restriction enzymes and hybridized to 13 nuclear, mitochondrial, and synthetic oligonucleotide DNA probes yielded 82 bands. Hybridization patterns indicated that the variation observed was minor. To further confirm the genetic fidelity, 12 arbitrary 10-base primers and six synthetic oligonucleotide sequences, successfully used to amplify genomic DNA from in vivo and in vitro materials, produced 133 fragments that were monomorphic across the plants tested. The present results demonstrate that enhanced-axillary-branching culture of mature trees could be utilized commercially for mass clonal propagation of these two important Eucalyptus species that have been recalcitrant to vegetative propagation. The results also provide novel insights into the genetic differences between E. tereticornis and E. camaldulensis.

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“…It is known that changes in DNA can occur during in vitro culture (Cecchini et al 1992;Zheng 1991;Puolimatka and Karp 1993).…”

Section: Resultsmentioning

confidence: 99%

Does 2,4-dichlorophenoxyacetic acid (2,4-D) induce genotoxic effects in tissue cultured Allium roots?

et al. 2016

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2,4-Dichlorophenoxyacetic acid (2,4-D) is a synthetic plant growth regulator that is highly toxic to most broad leaved plants and relatively nontoxic to monocotyledonous plants; is frequently used as weed killer. The study aimed to investigate cytogenetic effects of different concentrations of 2,4-D (0.67, 1.34, 2.01, 2.68, 3.35 and 4.02 mg/L) on Allium cepa bulblets' root tips treated for 24 and 48 h. The results showed six types of structural aberrations: C-mitosis, stickiness, laggards, bridges, fragments and multipolarity that varied numerically compared to control. It significantly affected mitotic index (MI) at 24 and 48 h treatment. In the Allium test, MI increased significantly at three lower concentrations (0.67, 1.34, 2.01 mg/L) after treatment with 2,4-D for 24 h and decreased significantly at higher concentration. Whereas, 2,4-D treatment for 48 h increased MI at all concentrations with significantly decreased MI at the highest concentration. The experiment was extended using comet test that did not reveal significant difference among treatments except for application of 4.02 mg/L 2,4-D for 48 h; where cell damages were verified by comet test. Rest of the concentrations for any duration of time were not damaging and toxic to cells. The results showed, visible mitodepressive action of 4.02 mg/L 2,4-D when treated for 48 h that had tendency to become toxic if the roots had been in touch with 2,4-D for a longer time.

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DNA variations in regenerated plants of pea (Pisum sativum L.)

Cited by 55 publications

References 31 publications

RETRACTED ARTICLE: Plant regeneration in Chlorophytum borivilianum Sant. et Fernand. from embryogenic callus and cell suspension culture and assessment of genetic fidelity of plants derived through somatic embryogenesis

RETRACTED ARTICLE: Plant regeneration in Chlorophytum borivilianum Sant. et Fernand. from embryogenic callus and cell suspension culture and assessment of genetic fidelity of plants derived through somatic embryogenesis

Genetic analysis of enhanced-axillary-branching-derived Eucalyptus tereticornis Smith and E. camaldulensis Dehn. plants

Does 2,4-dichlorophenoxyacetic acid (2,4-D) induce genotoxic effects in tissue cultured Allium roots?

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