DNA viruses and viral proteins that interact with PML nuclear bodies

Abstract

Connections between PML nuclear bodies (PML NBs) and DNA virus replication have been investigated from the earliest days of the molecular characterization of PML and associated proteins. It appears to be a general feature of nuclear-replicating DNA viruses that their parental genomes preferentially become associated with PML NBs, and that their initial sites of transcription and development of DNA replication centres are frequently juxtaposed to these domains or their remnants. In addition, regulatory proteins encoded by several DNA viruses associate with and sometimes cause catastrophic changes to PML NBs by a variety of mechanisms. These events can be correlated with the eciency of viral infection and the functions of viral regulatory proteins, but the underlying molecular connections between PML NB function and viral infection remain poorly understood. This article reviews the latest developments in the interactions between PML NBs and herpesviruses, adenoviruses and papovaviruses. Oncogene (2001) 20, 7266 ± 7273.

SUMO modification of E1B-55K oncoprotein regulates isoform-specific binding to the tumour suppressor protein PML

“…Furthermore, PML bodies are proposed to mediate a general intracellular barrier for viral infections (Everett, 2001;Everett and ChelbiAlix, 2007;Tavalai and Stamminger, 2008). Thus, it seems conclusive that adenoviruses evolved an E4orf3-dependent conserved mechanism to counteract this special PML-NB function (Stracker et al, 2005;Ullman et al, 2007;Ullman and Hearing, 2008).…”

“…Although the role of E1B-55K SUMOylation is still unclear, several line of evidence indicates that this posttranslational modification regulates the targeting of E1B-55K to subnuclear structures and viral transcription/replication centers (Endter et al, 2005;Kindsmu¨ller et al, 2007). Interestingly, PML as well as associated factors are suspected to mediate an intracellular viral defense mechanism and are targeted by multiple viral proteins during infection (Everett, 2001;Everett and Chelbi-Alix, 2007;Tavalai and Stamminger, 2008). For closer analysis of the role of PML during adenoviral infection/transformation, we decided to use HAdV5 E1B-55K as a tool to study the relationship of viral proteins with these nuclear structures and a putative involvement of PML in viral-mediated oncogenesis.…”

E2FBP1/hDril1 modulates cell growth through downregulation of promyelocytic leukemia bodies

“…[9][10][11] These provide a scaffold to harbor several prominent regulatory factors including tumor suppressors, transcriptional regulators, and enzymes for signal transduction, and thereby PML-NBs contribute to multiple mechanisms of cellular control. 8,10,[12][13][14][15][16] While the functional interactions of these components remain unclear, the architecture of PML-NBs seems to be particularly important since its abrogation caused by a chromosomal translocation t(15;17) results in acute promyelocytic leukemia. 8,14,16 In contrast, forced expression of PML, as well as the expression of oncogenic Ras, induces apparent hyperplasis (i.e.…”

Cross Talk between PML and p53 during Poliovirus Infection: Implications for Antiviral Defense

“…A fascinating aspect of PML and p53 is that both are directly induced by type I IFN (8,32,43), and viruses from different families encode proteins which counteract their localization and/or activity (11,15,33). In addition, p53 was recently shown to be involved in antiviral defense (43).…”